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    IMMUNOASSAYS INVOLVING SURFACE ENHANCED RAMAN SCATTERING
    1.
    发明公开
    IMMUNOASSAYS INVOLVING SURFACE ENHANCED RAMAN SCATTERING 有权
    免疫测定用SERS（表面增强拉曼散射）

    公开(公告)号：EP1062511A1

    公开(公告)日：2000-12-27

    申请号：EP99936121.5

    申请日：1999-02-26

    申请人： The University of Strathclyde

    发明人： WHITE, Peter Cyril ,  ATHMANI, Salah ,  SMITH, William, Ewen ,  SADLER, Daran Antony

    IPC分类号： G01N33/543 ,  G01N33/58 ,  G01N33/94

    CPC分类号： G01N33/587 ,  G01N33/54373 ,  G01N33/581 ,  G01N33/582 ,  G01N33/946 ,  Y10S436/801 ,  Y10S436/805 ,  Y10T436/24

    摘要： Disclosed are immunoassay displacement methods for detecting (preferably identifying) the presence or amount of a target analyte (e.g. drug) in a sample comprising the steps of: (a) exposing the sample to a complex comprising an immobilised antibody capable of specifically binding the analyte, said antibody being specifically bound to a displacement agent, wherein said agent comprises: (i) an analyte analog capable of specifically binding the antibody, (ii) a SE(R)RS active label, (iii) a SE(R)RS surface-seeking group (e.g. benztriazole), whereby any analyte present in the sample causes the displacement of agent from the antibody; (b) exposing any displaced agent to a SE(R)RS surface; and, (c) detecting any displaced agent associated with said surface using SE(R)RS. In preferred forms of the invention several target analytes are detected from a single source or sample simultaneously. Also provided are agents per se e.g. for use in drug or explosive detection. These may include chromophores and linking groups.

    Surface-enhanced raman spectroscopy immunoassy
    2.
    发明公开
    Surface-enhanced raman spectroscopy immunoassy 失效
    免疫测定手段Ramanspektroskopie unterverstärkenderOberflächenmitwirkung（SERS）。

    公开(公告)号：EP0587008A1

    公开(公告)日：1994-03-16

    申请号：EP93113836.6

    申请日：1993-08-30

    申请人： ABBOTT LABORATORIES

    发明人： Tarcha, Peter J. ,  Rohr, Thomas E. ,  Markese, James J. ,  Cotton, Therese ,  Rospendowski, Bernard

    IPC分类号： G01N33/553 ,  G01N21/65

    CPC分类号： G01N21/658 ,  G01N33/54373 ,  G01N2021/653 ,  Y10S435/968 ,  Y10S436/801 ,  Y10S436/805 ,  Y10T436/24

    摘要： A method, composition, device, apparatus, and kit for the determination of the presence or amount of an analyte by monitoring an analyte-mediated ligand binding event in a test mixture which contains the analyte to be assayed, a specific binding member, a Raman-active label, and a particulate having a surface capable of inducing a surface-enhanced Raman light scattering. The test mixture is illuminated with a radiation sufficient to cause the Raman-active label in the test mixture to emit a detectable Raman spectrum. The differences in the detected surface-enhanced Raman scattering spectra are dependent upon the amount of the analyte present in the test mixture. Thus, by monitoring these differences, the presence or amount of the analyte are determined.

    摘要翻译： 用于通过监测包含待测分析物的测试混合物中的分析物介导的配体结合事件来测定分析物的存在或量的方法，组合物，装置，装置和试剂盒，特异性结合成员，拉曼 活性标记物和具有能够诱导表面增强拉曼光散射的表面的颗粒。 用足以使测试混合物中的拉曼活性标记物发射可检测的拉曼光谱的辐射照射测试混合物。 检测到的表面增强拉曼散射光谱的差异取决于测试混合物中存在的分析物的量。 因此，通过监测这些差异，确定分析物的存在或量。

    Method for storage of labile protein materials
    8.
    发明公开
    Method for storage of labile protein materials 失效
    贮存不稳定蛋白质材料的方法

    公开(公告)号：EP0675361A3

    公开(公告)日：1995-10-25

    申请号：EP95110925.5

    申请日：1988-07-12

    申请人： Abbott Laboratories

    发明人： Ching, Shanfun ,  Billing, Patricia A. ,  Gordon, Julian

    IPC分类号： G01N33/543 ,  G01N33/558 ,  G01N33/531 ,  G01N33/58

    CPC分类号： G01N33/587 ,  G01N30/90 ,  G01N33/54366 ,  G01N33/54386 ,  G01N33/54393 ,  G01N33/558 ,  G01N33/585 ,  G01N33/76 ,  Y10S435/967 ,  Y10S435/97 ,  Y10S435/975 ,  Y10S436/801 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/826 ,  Y10S514/97

    摘要： The present invention relates to (1) a method for storing labile proteins which prevents aggregation and inactivation of the proteins when maintained in the dry state wherein said proteins may be rapidly solubilized, which method comprises drying the protein on a substrate in the presence of an aqueous medium containing a meta-soluble protein, (2) a product produced therefrom comprising a solid substrate upon which is impregnated and dried a labelled protein in the presence of an aqueous medium containing a meta-soluble protein and (3) a composition of matter comprising a colloidal particle labelled reagent and a sufficient amount of a meta-soluble protein to allow resolubilization from a dried state and chromatographic solvent transport of said colloidal particle labelled reagent.

    摘要翻译： 本发明涉及（1）用于储存不稳定蛋白质的方法，所述不稳定蛋白质当保持干燥状态时能够使蛋白质聚集和失活，其中所述蛋白质可以被快速溶解，该方法包括在存在 （2）由其制备的产品，其包含固体基质，在含有可溶性蛋白质的水性介质存在下，将标记的蛋白质浸渍并干燥，并且（3）物质组合物 其包含胶体颗粒标记的试剂和足够量的可溶性蛋白质以允许所述胶体颗粒标记的试剂从干燥状态重新溶解和色谱溶剂转移。

    A new universally applicable detection system based 0n ultra small colloidal metal particles
    10.
    发明公开
    A new universally applicable detection system based 0n ultra small colloidal metal particles 失效
    Auf ultrakleine kolloidale Metallteilchengegründetes，allgemein anwendbares Nachweissystem。

    公开(公告)号：EP0317001A1

    公开(公告)日：1989-05-24

    申请号：EP88202521.6

    申请日：1988-11-11

    申请人： JANSSEN PHARMACEUTICA N.V.

    发明人： Leunissen, Johannes L.M. ,  De Brabander, Marc J. ,  Van de Plas, Petrus F.E.M.

    IPC分类号： G01N33/553 ,  G01N33/58 ,  G01N33/532

    CPC分类号： G01N33/54346 ,  G01N33/553 ,  G01N33/587 ,  Y10S436/801 ,  Y10S530/811

    摘要： An aggregate for qualitatively or quantitatively determining bindable substances. Said aggregate comprises an ultra small colloidal particle and a specific binding agent characterized in that the mean diameter of the colloidal particle is below 2 nm. Preferably the size of the metal particle is selected so that the aggregate is capable of penetrating in standard biological specimens. A universally applicable detection system for determining bindable substances in particular in living material. Further there is provided a process for preparing said aggregate.

    摘要翻译： 用于定性或定量测定可结合物质的总量。 所述骨料包含超小胶体颗粒和特异性粘合剂，其特征在于胶体颗粒的平均直径低于2nm。 优选地选择金属颗粒的尺寸，使得聚集体能够在标准生物样品中渗透。 一种普遍适用的检测系统，特别用于确定生物材料中的可结合物质。 此外，提供了制备所述骨料的方法。