公开(公告)号：EP1383925B1

公开(公告)日：2006-09-20

申请号：EP02738211.8

申请日：2002-05-03

申请人： BIO MERIEUX ,  UNIVERSITE JOSEPH FOURIER (GRENOBLE 1) ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

发明人： BOURGET, Cécile ,  KOTERA, Mitsuharu ,  LHOMME, Jean ,  TREVISIOL, Emmanuelle ,  LAAYOUN, Ali ,  TORA, Christelle ,  SOTHIER, Isabelle

IPC分类号： C12Q1/68

CPC分类号： C12Q1/68

摘要： The invention concerns a method for labelling and fragmenting a single-stranded or double-stranded deoxyribonucleic acid (DNA) comprising the following steps: chemically fragmenting the DNA by generating an abasic site on said DNA, attaching a marker on at least one of the fragments by means of a labelling reagent, said reagent being covalently and majority bonded on at least one phosphate of said fragment. The invention is applicable in the field of diagnosis.

公开(公告)号：EP1383732B1

公开(公告)日：2010-02-17

申请号：EP02740788.1

申请日：2002-05-03

申请人： BIO MERIEUX ,  UNIVERSITE JOSEPH FOURIER (GRENOBLE 1) ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)

发明人： BOURGET, Cécile ,  LHOMME, Jean ,  LAAYOUN, Ali ,  KOTERA, Mitsuharu ,  TREVISIOL, Emmanuelle ,  MENOU, Lionel ,  BERNAL MENDEZ, Eloy

IPC分类号： C07C245/14 ,  C07C245/18 ,  C07D209/14 ,  C07D495/04 ,  C07D311/82 ,  C12Q1/68

CPC分类号： C07D209/08 ,  C07C245/14 ,  C07C245/18 ,  C07D311/90 ,  C07D495/04

公开(公告)号：EP1383925A2

公开(公告)日：2004-01-28

申请号：EP02738211.8

申请日：2002-05-03

申请人： BIO MERIEUX ,  UNIVERSITE JOSEPH FOURIER (GRENOBLE 1) ,  Centre National De La Recherche Scientifique

发明人： BOURGET, Cécile ,  KOTERA, Mitsuharu ,  LHOMME, Jean ,  TREVISIOL, Emmanuelle ,  LAAYOUN, Ali ,  TORA, Christelle ,  SOTHIER, Isabelle

IPC分类号： C12Q1/68

CPC分类号： C12Q1/68

摘要： The invention concerns a method for labelling and fragmenting a single-stranded or double-stranded deoxyribonucleic acid (DNA) comprising the following steps: chemically fragmenting the DNA by generating an abasic site on said DNA, attaching a marker on at least one of the fragments by means of a labelling reagent, said reagent being covalently and majority bonded on at least one phosphate of said fragment. The invention is applicable in the field of diagnosis.

公开(公告)号：EP1383732A1

公开(公告)日：2004-01-28

申请号：EP02740788.1

申请日：2002-05-03

申请人： BIO MERIEUX ,  UNIVERSITE JOSEPH FOURIER (GRENOBLE 1) ,  CENTRE NATIONAL DELA RECHERCHE SCIENTIFIQUE (CNRS)

发明人： BOURGET, Cécile ,  LHOMME, Jean ,  LAAYOUN, Ali ,  KOTERA, Mitsuharu ,  TREVISIOL, Emmanuelle ,  MENOU, Lionel ,  BERNAL MENDEZ, Eloy

IPC分类号： C07C245/14 ,  C07C245/18 ,  C07D209/14 ,  C07D495/04 ,  C07D311/82 ,  C12Q1/68

CPC分类号： C07D209/08 ,  C07C245/14 ,  C07C245/18 ,  C07D311/90 ,  C07D495/04

摘要： The invention concerns a temperature-stable labelling reagent of formula (I), wherein: R1 represents H or an alkyl, aryl or substituted aryl group; R2 represents a detectable marker or at least two detectable markers linked together by at least a multimeric structure; L is a linking arm comprising a linear catemer concatenation of at least two covalent bonds; and n is an integer equal to 0 or 1; R?3 and R4¿ represent, independently of each other: H, NO¿2?, Cl, Br, F, I, R?2-(L)¿n-Y-X-, OR, SR, NR2, R, NHCOR, CONHR, COOR with R = alkyl or aryl, A is a linking arm comprising at least a double bond enabling the conjugation of the diazo function with the aromatic cycle; and u is a whole number between 0 and 2, preferably between 0 and 1; and -Y-X- represents -CONH-, -NHCO-, -CH2O-, -CH2S-. The invention also concerns a method for the synthesis of said markers and uses for labelling biological molecules, in particular nucleic acids, with a labelling reagent bearing the diazomethyl function. The invention is particularly applicable in the field of diagnosis.