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1.发明授权REACTIFS DE MARQUAGE, PROCEDES DE SYNTHESE DE TELS REACTIFS ET PROCEDES DE DETECTION DE MOLECULES BIOLOGIQUES 有权标记试剂，处理此试剂用于检测生物分子的合成和方法
公开(公告)号：EP1383732B1
公开(公告)日：2010-02-17
申请号：EP02740788.1
申请日：2002-05-03
申请人： BIO MERIEUX , UNIVERSITE JOSEPH FOURIER (GRENOBLE 1) , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
发明人： BOURGET, Cécile , LHOMME, Jean , LAAYOUN, Ali , KOTERA, Mitsuharu , TREVISIOL, Emmanuelle , MENOU, Lionel , BERNAL MENDEZ, Eloy
IPC分类号： C07C245/14 , C07C245/18 , C07D209/14 , C07D495/04 , C07D311/82 , C12Q1/68
CPC分类号： C07D209/08 , C07C245/14 , C07C245/18 , C07D311/90 , C07D495/04
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公开(公告)号：EP1383925B1
公开(公告)日：2006-09-20
申请号：EP02738211.8
申请日：2002-05-03
申请人： BIO MERIEUX , UNIVERSITE JOSEPH FOURIER (GRENOBLE 1) , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
发明人： BOURGET, Cécile , KOTERA, Mitsuharu , LHOMME, Jean , TREVISIOL, Emmanuelle , LAAYOUN, Ali , TORA, Christelle , SOTHIER, Isabelle
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68
摘要： The invention concerns a method for labelling and fragmenting a single-stranded or double-stranded deoxyribonucleic acid (DNA) comprising the following steps: chemically fragmenting the DNA by generating an abasic site on said DNA, attaching a marker on at least one of the fragments by means of a labelling reagent, said reagent being covalently and majority bonded on at least one phosphate of said fragment. The invention is applicable in the field of diagnosis.
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公开(公告)号：EP1383925A2
公开(公告)日：2004-01-28
申请号：EP02738211.8
申请日：2002-05-03
申请人： BIO MERIEUX , UNIVERSITE JOSEPH FOURIER (GRENOBLE 1) , Centre National De La Recherche Scientifique
发明人： BOURGET, Cécile , KOTERA, Mitsuharu , LHOMME, Jean , TREVISIOL, Emmanuelle , LAAYOUN, Ali , TORA, Christelle , SOTHIER, Isabelle
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68
摘要： The invention concerns a method for labelling and fragmenting a single-stranded or double-stranded deoxyribonucleic acid (DNA) comprising the following steps: chemically fragmenting the DNA by generating an abasic site on said DNA, attaching a marker on at least one of the fragments by means of a labelling reagent, said reagent being covalently and majority bonded on at least one phosphate of said fragment. The invention is applicable in the field of diagnosis.
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4.发明公开REACTIFS DE MARQUAGE, PROCEDES DE SYNTHESE DE TELS REACTIFS ET PROCEDES DE DETECTION DE MOLECULES BIOLOGIQUES 有权标记试剂，处理此试剂用于检测生物分子的合成和方法
公开(公告)号：EP1383732A1
公开(公告)日：2004-01-28
申请号：EP02740788.1
申请日：2002-05-03
申请人： BIO MERIEUX , UNIVERSITE JOSEPH FOURIER (GRENOBLE 1) , CENTRE NATIONAL DELA RECHERCHE SCIENTIFIQUE (CNRS)
发明人： BOURGET, Cécile , LHOMME, Jean , LAAYOUN, Ali , KOTERA, Mitsuharu , TREVISIOL, Emmanuelle , MENOU, Lionel , BERNAL MENDEZ, Eloy
IPC分类号： C07C245/14 , C07C245/18 , C07D209/14 , C07D495/04 , C07D311/82 , C12Q1/68
CPC分类号： C07D209/08 , C07C245/14 , C07C245/18 , C07D311/90 , C07D495/04
摘要： The invention concerns a temperature-stable labelling reagent of formula (I), wherein: R1 represents H or an alkyl, aryl or substituted aryl group; R2 represents a detectable marker or at least two detectable markers linked together by at least a multimeric structure; L is a linking arm comprising a linear catemer concatenation of at least two covalent bonds; and n is an integer equal to 0 or 1; R?3 and R4¿ represent, independently of each other: H, NO¿2?, Cl, Br, F, I, R?2-(L)¿n-Y-X-, OR, SR, NR2, R, NHCOR, CONHR, COOR with R = alkyl or aryl, A is a linking arm comprising at least a double bond enabling the conjugation of the diazo function with the aromatic cycle; and u is a whole number between 0 and 2, preferably between 0 and 1; and -Y-X- represents -CONH-, -NHCO-, -CH2O-, -CH2S-. The invention also concerns a method for the synthesis of said markers and uses for labelling biological molecules, in particular nucleic acids, with a labelling reagent bearing the diazomethyl function. The invention is particularly applicable in the field of diagnosis.
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5.发明授权COMPOSE FONCTIONNALISE, POLYNUCLEOTIDE EVENTUELLEMENT MARQUE ET PROCEDE DE DETECTION D'UN ACIDE NUCLEIQUE CIBLE 有权功能化的连接，如果需要标记的多核苷酸和检测方法的靶核酸的
公开(公告)号：EP1140963B1
公开(公告)日：2004-09-22
申请号：EP00900527.3
申请日：2000-01-05
申请人： BIO MERIEUX
摘要： The invention concerns a functionalised compound of general formula (I) wherein: W represents a nucleotide analog; L represents a binding group comprising at least four atoms; R1 represents a linear or branched alkyl chain. The invention also concerns one said functionalised polynucleotide, one marked polynucleotide, including at least a functionalised compound corresponding to formula (I'). The invention further concerns a method for detecting a target nucleic acid using one said compound.
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公开(公告)号：EP0941314A1
公开(公告)日：1999-09-15
申请号：EP97936732.0
申请日：1997-08-01
申请人： BIO MERIEUX
发明人： GUILLOU-BONNICI, Françoise , DEFRANC, Eric , HOANG, Antoine , LAAYOUN, Ali , LHOMME, Jean , TREVISIOL, Emmanuelle
CPC分类号： C12P19/34 , C07H19/06 , C07H21/00 , C12N15/10 , C12P19/30 , C12Q1/686 , C12Q2527/125 , C12Q2525/101
摘要： A target nucleic acid sequence amplification method wherein at least the target nucleic acid sequence, at least one oligonucleotide primer specific for the target sequence, one or more enzymatic activities and nucleotides are provided, and the target sequence is amplified under conditions suitable in particular for said enzymatic or activity or activities, is disclosed. According to the method, at least one of the nucleotides is a prefunctionalised nucleotide that differs from the other nucleotides at least in that it includes at least one unprotected reactive covalence function in at least one predetermined site of the base of said nucleotide, in order to give a prefunctionalised amplification product including at least one such prefunctionalised nucleotide. Nucleotides for carrying out the method are also disclosed.
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7.发明公开COMPOSE FONCTIONNALISE, POLYNUCLEOTIDE EVENTUELLEMENT MARQUE ET PROCEDE DE DETECTION D'UN ACIDE NUCLEIQUE CIBLE 有权功能化的连接，如果需要标记的多核苷酸和检测方法的靶核酸的
公开(公告)号：EP1140963A2
公开(公告)日：2001-10-10
申请号：EP00900527.3
申请日：2000-01-05
申请人： BIO MERIEUX
摘要： The invention concerns a functionalised compound of general formula (I) wherein: W represents a nucleotide analog; L represents a binding group comprising at least four atoms; R1 represents a linear or branched alkyl chain. The invention also concerns one said functionalised polynucleotide, one marked polynucleotide, including at least a functionalised compound corresponding to formula (I'). The invention further concerns a method for detecting a target nucleic acid using one said compound.
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