利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

8 条记录 (耗时 0.017 秒)

    METHOD FOR MODIFICATION OF RECOMBINANT POLYPEPTIDES
    2.
    发明公开
    METHOD FOR MODIFICATION OF RECOMBINANT POLYPEPTIDES 失效
    方法进行修改重组多肽。

    公开(公告)号：EP0651761A1

    公开(公告)日：1995-05-10

    申请号：EP93918187.0

    申请日：1993-07-13

    申请人： BIONEBRASKA, INC.

    发明人： STOUT, Jay ,  WAGNER, Fred W. ,  COOLIDGE, Thomas, R. ,  HOLMQUIST, Bart

    IPC分类号： C12N15 ,  C07K1 ,  C07K5 ,  C07K7 ,  C07K14 ,  C07K19 ,  C12P21

    CPC分类号： C07K14/60 ,  C07K1/003 ,  C07K1/006 ,  C07K1/1072 ,  C07K1/1075 ,  C07K1/12 ,  C07K5/1019 ,  C07K7/18 ,  C07K14/4716 ,  C07K14/475 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/75 ,  C12N15/62 ,  Y02P20/55

    摘要： The invention provides for a chemical method for preparing a recombinant single copy polypeptide or a portion thereof with a modified terminal amino acid α-carbon reactive group selected from the group consisting of N-terminal α-amine, C-terminal α-carboxyl, and a combination thereof. The steps of the method involve forming the recombinant single copy polypeptide or a portion thereof so that the single copy polypeptide is protected with one or more biologically added protecting groups at the N-terminal α-amine, C-terminal α-carboxyl. The recombinant single copy polypeptide can then be reacted with up to three chemical protecting agents to selectively protect reactive side chain groups and thereby prevent side chain groups from being modified. The recombinant single copy polypeptide can be cleaved with at least one cleavage reagent specific for the biological protecting group to form an unprotected terminal amino acid α-carbon reactive group. The unprotected terminal amino acid α-carbon reactive group is modified with at least one chemical modifying agent. The side chain protected terminally modified single copy polypeptide is then deprotected at the side chain groups to form a terminally modified recombinant single copy polypeptide. The number and sequence of steps in the method can be varied to achieve selective modification at the N- and/or C-terminal amino acid of a recombinantly produced polypeptide.

    PURIFICATION DIRECTED CLONING OF PEPTIDES
    4.
    发明公开
    PURIFICATION DIRECTED CLONING OF PEPTIDES 失效
    用于清洁肽某些克隆。

    公开(公告)号：EP0539530A1

    公开(公告)日：1993-05-05

    申请号：EP92902529.0

    申请日：1991-06-24

    申请人： BIONEBRASKA, INC.

    发明人： COOLIDGE, Thomas, R. ,  WAGNER, Fred ,  VAN HEEKE, Gino ,  SCHUSTER, Sheldon, M. ,  STOUT, Jay ,  WYLIE, Dwane, E.

    IPC分类号： C07K14 ,  C07K1 ,  C07K7 ,  C07K19 ,  C12N1 ,  C12N9 ,  C12N15 ,  C12P21 ,  C12R1

    CPC分类号： C12N9/14 ,  C07K7/14 ,  C07K14/245 ,  C07K14/47 ,  C07K14/585 ,  C07K14/595 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/75 ,  C12N9/88 ,  C12N15/62

    摘要： Cette invention concerne des procédés utilisant des microbes et des organismes plus évolués pour l'expression et la purification par affinité immobilisée par ligand d'un produit peptidique. Les compositions biologiques de cette invention qui sont réalisées à l'aide de ces procédés contiennent ou bien sont destinées à exprimer une structure de purification de protéine, ayant trois segments couplés en tandem composés d'une protéine de liaison, d'un peptide d'interconnexion et d'un polypeptide à fusion variable qui incorpore le produit peptidique dans une ou plusieurs copies. Suivant le procédé de l'invention, on obtient le produit peptidique comme suit: (a) on exprime une cellule hôte ou un organisme hôte de manière régulée par le vecteur d'expression recombinant portant le gène recombinant codant pour la structure de purification de protéine; (b) on purifie la structure de purification des protéines à l'aide d'une technique de séparation par affinité immobilisée par ligand; et (c) on effectue le clivage chimique ou enzymatique de la structure de purification de protéine pour produire le produit peptidique.

    ENZYMATIC METHOD FOR MODIFICATION OF RECOMBINANT POLYPEPTIDES
    8.
    发明公开
    ENZYMATIC METHOD FOR MODIFICATION OF RECOMBINANT POLYPEPTIDES 失效
    方法的重组多肽的酶法改性

    公开(公告)号：EP0789760A2

    公开(公告)日：1997-08-20

    申请号：EP94931264.0

    申请日：1994-07-19

    申请人： BIONEBRASKA, INC.

    发明人： WAGNER, Fred, W. ,  STOUT, Jay ,  HENRIKSEN, Dennis ,  PARTRIDGE, Bruce ,  MANNING, Shane

    IPC分类号： C12N15 ,  A61K38 ,  A61P3 ,  A61P13 ,  A61P15 ,  A61P43 ,  C07K14 ,  C12N1 ,  C12P21 ,  C12R1

    CPC分类号： C07K14/60 ,  A61K38/00 ,  C07K14/605 ,  C07K2319/00 ,  C12P21/02 ,  C12P21/06

    摘要： The method of the invention provides for the formation of a recombinant polypeptide which has been modified at the C-terminal end through the use of a transpeptidation process. The method is suitable for modifying recombinant polypeptides of any source including those which may be commercially available, those derived from recombinant single copy or multicopy polypeptide constructs, or those derived from single or multicopy recombinant fusion protein constructs. The transpeptidation reaction involves contacting an endopeptidase enzyme with a recombinant polypeptide to substitute an addition unit, of one or more amino acids, for a leaving unit, linked to a core polypeptide through a cleavage site recognized by the endopeptidase enzyme. Recombinant polypeptides derived from multicopy polypeptide constructs may be cleaved from the multicopy polypeptide at the N-terminal and C-terminal ends and simultaneously undergo substitution of the leaving unit by the desired addition unit. The invention utilizes known and newly discovered cleavage recognition sites to effectuate the desired modification products.