公开(公告)号：EP1237926A4

公开(公告)日：2006-04-12

申请号：EP00983939

申请日：2000-12-06

申请人： BIOSITE INC ,  GENPHARM INT

发明人： BUECHLER JOE ,  VALKIRS GUNARS ,  GRAY JEFF ,  LONBERG NILS

IPC分类号： C07K16/18 ,  C07K16/24 ,  C12N15/09 ,  G01N33/53 ,  G01N33/543 ,  C07K16/00

CPC分类号： C07K16/18 ,  C07K16/244 ,  C07K2317/21 ,  C07K2317/55 ,  G01N33/543 ,  Y10S435/962 ,  Y10S435/973 ,  Y10S436/809

摘要： The invention provides in vitro methods of detection using human antibodies. The methods are particularly useful for analyzing human samples containing HAMA or heterophilic antibodies. A human antibody can bind to an analyte in such samples without binding to HAMA or heterophilic antibodies present in the samples. The methods can be effected using a sandwich format among others.

公开(公告)号：EP0746609A4

公开(公告)日：1997-12-17

申请号：EP93901143

申请日：1992-12-17

申请人： GENPHARM INT

发明人： LONBERG NILS ,  KAY ROBERT M

IPC分类号： A01K67/027 ,  A61K38/00 ,  C07K16/00 ,  C07K16/28 ,  C07K16/30 ,  C07K16/42 ,  C07K16/44 ,  C12N5/10 ,  C12N15/02 ,  C12N15/09 ,  C12N15/85 ,  C12N15/90 ,  C12P21/08 ,  C12R1/91 ,  C12N15/00

CPC分类号： A01K67/0276 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  A01K2267/0381 ,  A61K38/00 ,  C07K16/00 ,  C07K16/2812 ,  C07K16/3007 ,  C07K16/4291 ,  C07K16/44 ,  C07K2317/21 ,  C07K2317/24 ,  C12N15/8509 ,  C12N15/90

摘要： The invention relates to transgenic non-human animals capable of producing heterologous antibodies and transgenic non-human animals having inactivated endogenous immunoglobulin genes. In one aspect of the invention, endogenous immunoglobulin genes are suppresed by antisense polynucleotides and/or by antiserum directed against endogenous immunoglobulins. Heterologous antibodies are encoded by immunoglobulin genes not normally found in the genome of that species of non-human animal. In one aspect of the invention, one or more transgenes containing sequences of unrearranged heterogolous human immunoglobulin heavy chains are introduced into a non-human animal thereby forming a transgenic animal capable of functionally rearranging transgenic immunoglobulin sequences and producing a repertoire of antibodies of various isotypes encoded by human immunoglobulin genes. Such heterologous human antibodies are produced in B-cells which are thereafter immortalized, e.g., by fusing with an immortalizing cell line such as a myeloma or by manipulating such B-cells by other techniques to perpetuate a cell line capable of producing a monoclonal heterologous antibody. The invention also relates to heavy and light chain immunoglobulin transgenes for making such transgenic non-human animals as well as methods and vectors for disrupting endogenous immunoglobulin loci in the transgenic animal.

公开(公告)号：EP0814159A2

公开(公告)日：1997-12-29

申请号：EP97201755

申请日：1991-08-28

申请人： GENPHARM INT

发明人： LONBERG NILS ,  KAY ROBERT

IPC分类号： A01K67/027 ,  A61K38/00 ,  A61K39/395 ,  C07K14/725 ,  C07K16/00 ,  C07K16/18 ,  C07K16/28 ,  C07K16/30 ,  C07K16/42 ,  C07K16/44 ,  C07K16/46 ,  C12N5/10 ,  C12N5/16 ,  C12N15/00 ,  C12N15/09 ,  C12N15/13 ,  C12N15/85 ,  C12N15/87 ,  C12N15/90 ,  C12P21/08 ,  C12N5/06

CPC分类号： C12N15/907 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/01 ,  A61K38/00 ,  C07K14/7051 ,  C07K16/00 ,  C07K16/2812 ,  C07K16/3007 ,  C07K16/4266 ,  C07K16/4291 ,  C07K16/44 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/56 ,  C12N5/163 ,  C12N15/00 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/87 ,  C12N15/90 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/36 ,  C12N2830/60 ,  C12N2840/44

摘要： A transgenic mouse having a genome comprising a human immunoglobulin heavy chain minilocus transgene, the transgene being capable of rearrangement and switching in the mouse, whereby two or more rearranged human heavy chain isotypes are produced.

摘要翻译： 一种具有包含人免疫球蛋白重链微型转基因的基因组的转基因小鼠，所述转基因能够在小鼠中重排和切换，由此产生两个或更多个重排的人重链同种型。

公开(公告)号：EP0854917A4

公开(公告)日：2002-07-24

申请号：EP96941938

申请日：1996-10-10

申请人： GENPHARM INT

发明人： LONBERG NILS ,  KAY ROBERT M

IPC分类号： C12N15/02 ,  A01K67/027 ,  A61K38/00 ,  C07K14/725 ,  C07K16/00 ,  C07K16/28 ,  C07K16/30 ,  C07K16/42 ,  C07K16/44 ,  C07K16/46 ,  C12N5/00 ,  C12N5/10 ,  C12N5/16 ,  C12N15/00 ,  C12N15/09 ,  C12N15/113 ,  C12N15/85 ,  C12N15/87 ,  C12N15/90 ,  C12P21/08 ,  C12R1/91

CPC分类号： C12N15/8509 ,  A01K67/0276 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/0368 ,  A61K38/00 ,  C07K14/7051 ,  C07K16/00 ,  C07K16/2812 ,  C07K16/3007 ,  C07K16/4266 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/92 ,  C07K2319/00 ,  C12N5/163 ,  C12N15/00 ,  C12N15/113 ,  C12N15/85 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2310/111 ,  C12N2800/206 ,  C12N2800/70 ,  C12N2830/30 ,  C12N2830/36 ,  C12N2830/85

摘要： The invention relates to transgenic non-human animals capable of producing heterologous antibodies and methods for producing human sequence antibodies which bind to human antigens with substantial affinity.

公开(公告)号：EP0754225A4

公开(公告)日：2001-01-31

申请号：EP94916581

申请日：1994-04-25

申请人： GENPHARM INT

发明人： LONBERG NILS ,  KAY ROBERT M

IPC分类号： A01K67/027 ,  A61K38/00 ,  C07K16/00 ,  C07K16/28 ,  C07K16/30 ,  C07K16/42 ,  C07K16/44 ,  C07K16/46 ,  C12N5/10 ,  C12N15/02 ,  C12N15/85 ,  C12P21/08 ,  C12N15/13 ,  A61K39/00

CPC分类号： C12N15/8509 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/30 ,  A01K2227/105 ,  A01K2267/01 ,  A61K38/00 ,  C07K16/00 ,  C07K16/2812 ,  C07K16/3007 ,  C07K16/4291 ,  C07K16/44 ,  C07K16/462 ,  C07K2317/24

摘要： The invention relates to transgenic non-human animals capable of producing heterologous antibodies and methods for producing human sequence antibodies which bind to human antigens with substantial affinity.

公开(公告)号：EP1222314A4

公开(公告)日：2006-07-12

申请号：EP00968612

申请日：2000-10-02

申请人： BIOSITE INC ,  GENPHARM INTERNAT A SUBSIDIARY

发明人： BUECHLER JOE ,  VALKIRS GUNARS ,  GRAY JEFF ,  LONBERG NILS ,  FLORES BECKY MAR

IPC分类号： A01K67/027 ,  C07K16/00 ,  C07K16/18 ,  C07K16/24 ,  C12N15/09 ,  C12N15/10 ,  C12N15/85 ,  C12P19/34 ,  C12P21/08 ,  C40B40/02 ,  C12Q1/70 ,  A01K67/00 ,  C07H21/04 ,  C12N5/06 ,  C12N5/16 ,  C12N15/00 ,  C12N15/64 ,  C12N15/74 ,  C12P21/06 ,  C12Q1/68 ,  G01N33/53

CPC分类号： C40B40/02 ,  A01K67/0275 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/01 ,  C07K16/00 ,  C07K16/244 ,  C07K2317/21 ,  C12N15/1037 ,  C12N15/8509

摘要： The invention uses the power of display selection methods to screen libraries of human immunoglobulin genes from nonhuman transgenic animals expressing human immunoglobulins. Such screening produces unlimited numbers of high affinity human antibodies to any target of interest.