公开(公告)号：EP2818543A4

公开(公告)日：2015-11-04

申请号：EP12869142

申请日：2012-11-29

申请人： BLOOMAGE FREDA BIOPHARM CO LTD

发明人： GUO XUEPING ,  SHI YANLI ,  QIAO LIPING ,  FENG NING ,  WANG GUANFENG ,  LI HAINA ,  XUE WEI ,  WANG HAIYING ,  LUAN YIHONG ,  LIU AIHUA

IPC分类号： C12N1/20 ,  A61K8/73 ,  A61K31/728 ,  A61P9/00 ,  A61P17/02 ,  A61Q17/00 ,  C07K14/32 ,  C12N9/26 ,  C12N15/56 ,  C12P19/28 ,  C12R1/07

CPC分类号： C12N9/2474 ,  A23L33/17 ,  A23V2002/00 ,  A61K8/66 ,  A61K8/735 ,  A61K31/728 ,  A61Q17/04 ,  A61Q19/00 ,  A61Q19/004 ,  A61Q19/02 ,  A61Q19/08 ,  C08B37/0072 ,  C12N9/2408 ,  C12P19/26 ,  C12P19/28 ,  C12R1/07 ,  Y02P20/52

摘要： The present invention provides a bacillus sp. having a deposit access number of CGMCC NO. 5744 and a hyaluronidase produced by the bacillus and the amino acid sequence of the hyaluronidase is shown in SEQ ID NO: 2. The present invention further relates to a process for preparing oligomeric hyaluronic acid or salts thereof or low-molecular-weight hyaluronic acid or salts thereof by using the bacillus or the hyaluronidase produced thereby. The produced oligomeric hyaluronates or low-molecular-weight hyaluronates have advantages such as good transdermal absorption ability, high purity, no cytotoxicity, potent antioxidant ability. The present invention also provides use of the bacillus having a deposit access number of CGMCC NO. 5744, or the hyaluronidase, oligomeric hyaluronates or salts thereof, low-molecular-weight hyaluronates or salts thereof produced by the bacillus in the fields of osmetics, food products and medicines.