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1.发明公开MICROFLUIDIC MANIPULATION OF FLUIDS AND REACTIONS 审中-公开MIKROFLUIDHANDHABUNG VON FLUIDEN UND REAKENEN
公开(公告)号：EP1928666A2
公开(公告)日：2008-06-11
申请号：EP06803022.0
申请日：2006-09-07
IPC分类号： B41J2/17
CPC分类号： B01L3/502784 , B01L3/06 , B01L3/502792 , B01L2200/0642 , B01L2200/0673 , B01L2200/10 , B01L2200/14 , B01L2200/141 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2400/0487 , Y10T156/1093 , Y10T436/11 , Y10T436/2575
摘要： The present invention relates generally to microfluidic structures, and more specifically, to microfluidic structures and methods including microreactors for manipulating fluids and reactions. In some embodiments, structures and methods for manipulating many (e.g., 1000) fluid samples, i.e., in the form of droplets, are described. Processes such as diffusion, evaporation, dilution, and precipitation can be controlled in each fluid sample. These methods also enable conditions within the fluid samples (e.g., concentration) to be controlled. Manipulation of fluid samples can be useful for a variety of applications, including testing for reaction conditions, e.g., in crystallization, chemical, and biological assays.
摘要翻译： 本发明一般涉及微流体结构，更具体地涉及微流体结构和方法，包括用于操纵流体和反应的微反应器。 在一些实施例中，描述了用于操纵许多（例如，1000）流体样品，即液滴形式的结构和方法。 可以在每个流体样品中控制扩散，蒸发，稀释和沉淀等过程。 这些方法还使流体样品中的条件（例如浓度）得以控制。 流体样品的操作可用于各种应用，包括测试反应条件，例如在结晶，化学和生物测定中。
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公开(公告)号：EP3536396A1
公开(公告)日：2019-09-11
申请号：EP19162019.4
申请日：2007-08-07
申请人： The President and Fellows of Harvard College , UNIVERSITE LOUIS PASTEUR DE STRASBOURG , Bio-Rad Laboratories, Inc. , Centre National de la Recherche Scientifique (C.N.R.S.)
发明人： HOLTZE, Christian , GUERRA, Rodrigo E. , AGRESTI, Jeremy , WEITZ, David A. , AHN, Keunho , HUTCHISON, John Brian , GRIFFITHS, Andrew S , EL HARRAK, Abdeslam , MILLER, Oliver, Jon , BARET, Jean-Christophe , TALY, Valérie , RYCHELLYNCK, Michaël , MERTEN, Christoph
摘要： Surfactants (e.g., fluorosurfactants) for stabilizing aqueous or hydrocarbon droplets in a fluorophilic continuous phase are presented. In some embodiments, fluorosurfactants include a fluorophilic tail soluble in a fluorophilic (e.g., fluorocarbon) continuous phase, and a headgroup soluble in either an aqueous phase or a lipophilic (e.g., hydrocarbon) phase. The combination of a fluorophilic tail and a headgroup may be chosen so as to create a surfactant with a suitable geometry for forming stabilized reverse emulsion droplets having a disperse aqueous or lipophilic phase in a continuous, fluorophilic phase. In some embodiments, the headgroup is preferably non-ionic and can prevent or limit the adsorption of molecules at the interface between the surfactant and the discontinuous phase. This configuration can allow the droplet to serve, for example, as a reaction site for certain chemical and/or biological reactions. In another embodiment, aqueous droplets are stabilized in a fluorocarbon phase at least in part by the electrostatic attraction of two oppositely charged or polar components, one of which is at least partially soluble in the dispersed phase, the other at least partially soluble in the continuous phase. One component may provide colloidal stability of the emulsion, and the other may prevent the adsorption of biomolecules at the interface between a component and the discontinuous phase. Advantageously, surfactants and surfactant combinations of the invention may provide sufficient stabilization against coalescence of droplets, without interfering with processes that can be carried out inside the droplets.
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公开(公告)号：EP2077912B1
公开(公告)日：2019-03-27
申请号：EP07836615.0
申请日：2007-08-07
申请人： The President and Fellows of Harvard College , UNIVERSITE LOUIS PASTEUR DE STRASBOURG , Raindance Technologies, Inc. , Centre National de la Recherche Scientifique (C.N.R.S.)
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4.发明公开
公开(公告)号：EP2077912A1
公开(公告)日：2009-07-15
申请号：EP07836615.0
申请日：2007-08-07
申请人： The President and Fellows of Harvard College , UNIVERSITE LOUIS PASTEUR DE STRASBOURG , Raindance Technologies, Inc. , Le Centre National De La Recherche Scientifique (CNRS)
发明人： HOLTZE, Christian , GUERRA, Rodrigo E. , AGRESTI, Jeremy , WEITZ, David A. , AHN, Kuenho , HUTCHISON, John Brian, , GRIFFITHS, Andrews , EL HARRAK, Abdeslam, , MILLER, Oliver, Jon, , BARET, Jean-Christophe, , TALY, Valérie, , RYCHELLYNCK, Micheal, , MERTEN, Christoph,
CPC分类号： B01F17/005 , B01F17/0035 , C08G65/007 , C08G65/332 , C08G65/337 , C08G81/00 , C08G2650/48 , C08L71/00 , C08L2201/52 , C08L2205/05
摘要： Surfactants (e.g., fluorosurfactants) for stabilizing aqueous or hydrocarbon droplets in a fluorophilic continuous phase are presented. In some embodiments, fluorosurfactants include a fluorophilic tail soluble in a fluorophilic (e.g., fluorocarbon) continuous phase, and a headgroup soluble in either an aqueous phase or a lipophilic (e.g., hydrocarbon) phase. The combination of a fluorophilic tail and a headgroup may be chosen so as to create a surfactant with a suitable geometry for forming stabilized reverse emulsion droplets having a disperse aqueous or lipophilic phase in a continuous, fluorophilic phase. In some embodiments, the headgroup is preferably non-ionic and can prevent or limit the adsorption of molecules at the interface between the surfactant and the discontinuous phase. This configuration can allow the droplet to serve, for example, as a reaction site for certain chemical and/or biological reactions. In another embodiment, aqueous droplets are stabilized in a fluorocarbon phase at least in part by the electrostatic attraction of two oppositely charged or polar components, one of which is at least partially soluble in the dispersed phase, the other at least partially soluble in the continuous phase. One component may provide collodial stability of the emulsion, and the other may prevent the adsorption of biomolecules at the interface between a component and the discontinous phase. Advantageously, surfactants and surfactant combinations of the invention may provide sufficient stabilization against coalescence of droplets, without interfering with processes that can be carried out inside the droplets.
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