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公开(公告)号：EP3536396A1
公开(公告)日：2019-09-11
申请号：EP19162019.4
申请日：2007-08-07
申请人： The President and Fellows of Harvard College , UNIVERSITE LOUIS PASTEUR DE STRASBOURG , Bio-Rad Laboratories, Inc. , Centre National de la Recherche Scientifique (C.N.R.S.)
发明人： HOLTZE, Christian , GUERRA, Rodrigo E. , AGRESTI, Jeremy , WEITZ, David A. , AHN, Keunho , HUTCHISON, John Brian , GRIFFITHS, Andrew S , EL HARRAK, Abdeslam , MILLER, Oliver, Jon , BARET, Jean-Christophe , TALY, Valérie , RYCHELLYNCK, Michaël , MERTEN, Christoph
摘要： Surfactants (e.g., fluorosurfactants) for stabilizing aqueous or hydrocarbon droplets in a fluorophilic continuous phase are presented. In some embodiments, fluorosurfactants include a fluorophilic tail soluble in a fluorophilic (e.g., fluorocarbon) continuous phase, and a headgroup soluble in either an aqueous phase or a lipophilic (e.g., hydrocarbon) phase. The combination of a fluorophilic tail and a headgroup may be chosen so as to create a surfactant with a suitable geometry for forming stabilized reverse emulsion droplets having a disperse aqueous or lipophilic phase in a continuous, fluorophilic phase. In some embodiments, the headgroup is preferably non-ionic and can prevent or limit the adsorption of molecules at the interface between the surfactant and the discontinuous phase. This configuration can allow the droplet to serve, for example, as a reaction site for certain chemical and/or biological reactions. In another embodiment, aqueous droplets are stabilized in a fluorocarbon phase at least in part by the electrostatic attraction of two oppositely charged or polar components, one of which is at least partially soluble in the dispersed phase, the other at least partially soluble in the continuous phase. One component may provide colloidal stability of the emulsion, and the other may prevent the adsorption of biomolecules at the interface between a component and the discontinuous phase. Advantageously, surfactants and surfactant combinations of the invention may provide sufficient stabilization against coalescence of droplets, without interfering with processes that can be carried out inside the droplets.
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公开(公告)号：EP2077912B1
公开(公告)日：2019-03-27
申请号：EP07836615.0
申请日：2007-08-07
申请人： The President and Fellows of Harvard College , UNIVERSITE LOUIS PASTEUR DE STRASBOURG , Raindance Technologies, Inc. , Centre National de la Recherche Scientifique (C.N.R.S.)
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公开(公告)号：EP1805324B1
公开(公告)日：2011-10-05
申请号：EP05792245.2
申请日：2005-10-10
发明人： GRIFFITHS, Andrew , WEITZ, David , LINK, Darren , AHN, Keunho , BIBETTE, Jerome
IPC分类号： C12Q1/68
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desire activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
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公开(公告)号：EP2004316B1
公开(公告)日：2010-10-13
申请号：EP07717014.0
申请日：2007-01-24
CPC分类号： B01F13/0071 , B01F13/0076 , B01J19/0093 , B01J2219/00272 , B01J2219/00828 , B01J2219/00833 , B01J2219/00835 , B01J2219/00853 , B01J2219/00889 , B01J2219/0093 , B01L3/5027 , B01L3/502784 , B01L2200/0673 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , G01N15/1404 , G01N2015/1006
摘要： The present invention relates to a method, comprising: providing a microfluidic system comprising one or more channels (25,28,29); providing within said one or more channels (25,28,29) a first fluidic stream and a second fluidic stream, at least one of which comprises droplets that have an average diameter of less than about 450 microns and that are highly monodisperse; and applying an electric field (40) simultaneously to at least a portion of the first fluidic stream and a portion of the second fluidic stream such that said portions coalesce.
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公开(公告)号：EP1812156A1
公开(公告)日：2007-08-01
申请号：EP05792864.0
申请日：2005-10-12
发明人： GRIFFITHS, Andrew , WEITZ, David , LINK, Darren , AHN, Keunho , BIBETTE, Jerome
IPC分类号： B01J19/00 , G01N33/543 , C07K1/04
CPC分类号： B01F13/0071 , B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0076 , B01J19/0046 , B01J2219/00497 , B01J2219/005 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00599 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01J2219/00743 , B01J2219/00853 , B01J2219/0086 , B01J2219/00889 , B01L3/5025 , B01L3/50273 , B01L3/502746 , B01L3/502761 , B01L3/502784 , B01L2200/0605 , B01L2200/0673 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , B82Y10/00 , B82Y30/00 , C07K1/047 , C40B40/06 , C40B40/10 , C40B50/08 , G01N2035/1034
摘要： The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalising two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control. The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalised into the microcapsules.
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公开(公告)号：EP1805324A2
公开(公告)日：2007-07-11
申请号：EP05792245.2
申请日：2005-10-10
发明人： GRIFFITHS, Andrew Medical Research Council , WEITZ, David , LINK, Darren , AHN, Keunho , BIBETTE, Jerome
IPC分类号： C12Q1/68
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desire activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
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公开(公告)号：EP2004316A2
公开(公告)日：2008-12-24
申请号：EP07717014.0
申请日：2007-01-24
CPC分类号： B01F13/0071 , B01F13/0076 , B01J19/0093 , B01J2219/00272 , B01J2219/00828 , B01J2219/00833 , B01J2219/00835 , B01J2219/00853 , B01J2219/00889 , B01J2219/0093 , B01L3/5027 , B01L3/502784 , B01L2200/0673 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , G01N15/1404 , G01N2015/1006
摘要： The present invention relates to a method, comprising: providing a microfluidic system comprising one or more channels (25,28,29); providing within said one or more channels (25,28,29) a first fluidic stream and a second fluidic stream, at least one of which comprises droplets that have an average diameter of less than about 450 microns and that are highly monodisperse; and applying an electric field (40) simultaneously to at least a portion of the first fluidic stream and a portion of the second fluidic stream such that said portions coalesce.
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