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1.发明授权FORMULATION OF SUGAR SOLUTIONS FOR CONTINUOUS ULTRACENTRIFUGATION FOR VIRUS PURIFICATION 有权糖溶液进行不断的超速离心进行病毒清洁制剂
公开(公告)号：EP2155864B1
公开(公告)日：2013-07-17
申请号：EP08749278.1
申请日：2008-04-30
发明人： REITER, Manfred , GRILLBERGER, Leopold , MUNDT, Wolfgang , MITTERER, Artur , SCHAFHAUSER, Horst
CPC分类号： C12N7/00 , A61K39/00 , A61K39/12 , A61K39/145 , C12N2750/14351 , C12N2760/16134 , C12N2760/16151 , C12N2760/16152 , C12N2760/16234 , C12N2760/16251 , C12N2760/16252
摘要： The present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by the addition of two or more buffered sugar layers of different concentration. The method leads to higher yields and reduces unwanted aggregation of the virus or virus antigen by increasing the volume of the peak pool.
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2.发明公开METHOD OF CONCENTRATING SHEAR-SENSITIVE BIOPOLYMERS USING HOLLOW FIBRE MEMBRANES 审中-公开集中剪切敏感的生物聚合物的方法采用中空纤维膜
公开(公告)号：EP2334411A1
公开(公告)日：2011-06-22
申请号：EP09792008.6
申请日：2009-08-27
CPC分类号： B01D61/243 , B01D2311/165 , B01D2325/04
摘要： The invention relates generally to methods of concentrating mixtures including shear sensitive biopolymers, such as von Willebrand Factor. Conventional methods of concentrating biopolymers impart too much shear stress, which causes the degradation of shear sensitive biopolymers. The methods disclosed herein reduce the shear stress while maintaining a high rate of filtrate flux. Disclosed herein is a method for concentrating shear sensitive biopolymers including flowing a mixture with a shear sensitive biopolymer into a hollow fiber dialysis module to form a retentate having a shear sensitive biopolymer concentration that is greater than that of the mixture. Hollow fiber dialysis modules have high filtrate fluxes and low shear rates at low flow rates. This ensures a high product yield and minimal loss of shear sensitive biopolymers.
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3.发明公开PURIFICATION METHOD FOR DIVALENT CATION BINDING PROTEINS ON ANION EXCHANGE RESIN 审中-公开纯化PROCESS FOR结合蛋白BIVALENTE阳离子的阴离子交换树脂
公开(公告)号：EP2563805A1
公开(公告)日：2013-03-06
申请号：EP11716431.9
申请日：2011-04-29
摘要： The present invention relates to a method for the purification of divalent cation binding proteins with high purity on an anion exchange resin material, to divalent cation binding proteins obtainable by said method, and to a kit comprising means for carrying out said method.
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4.发明公开PURIFICATION OF VWF FOR INCREASED REMOVAL OF NON-LIPID ENVELOPED VIRUSES 有权除去未脂质的增加的vWF的Aufrenigung包膜病毒
公开(公告)号：EP2467399A1
公开(公告)日：2012-06-27
申请号：EP10747134.4
申请日：2010-08-20
IPC分类号： C07K14/755 , A61K38/36
CPC分类号： C07K14/755 , A61K38/00 , C07K1/18
摘要： The present invention provides methods for purifying Von Willebrand factor (VWF) for increased removal of non-lipid enveloped viruses.
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公开(公告)号：EP2152735A1
公开(公告)日：2010-02-17
申请号：EP08754504.2
申请日：2008-05-16
IPC分类号： C07K14/435
CPC分类号： C07K14/435
摘要： The present invention relates to a method for producing a mature von Willebrand Factor (VWF) from von Willebrand Factor pro-peptide comprising the steps: - immobilizing VWF pro-peptide on an ion exchange resin, incubating the immobilized VWF pro-peptide with furin to obtain immobilized mature VWF, and - isolating mature VWF from the ion exchange resin by elution.
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公开(公告)号：EP2152735B1
公开(公告)日：2015-03-18
申请号：EP08754504.2
申请日：2008-05-16
IPC分类号： C07K14/435
CPC分类号： C07K14/435
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7.发明公开SUBSTANTIALLY ANIMAL PROTEIN-FREE RECOMBINANT FURIN AND METHODS FOR PRODUCING SAME 审中-公开WEITGEHEND TIERPROTEINFREIES REKOMBINANTES FURIN UND HERSTELLUNGSVERFAHRENDAFÜR
公开(公告)号：EP2240578A1
公开(公告)日：2010-10-20
申请号：EP08869658.8
申请日：2008-12-19
发明人： PLAIMAUER, Barbara , VON FIRCKS, Simone , GRILLBERGER, Leopold , HASSLACHER, Meinhard , GEYER, Roland , MITTERER, Artur , REITER, Manfred
IPC分类号： C12N9/64 , C07K14/755 , C12P21/02
CPC分类号： C12N9/6454
摘要： The present invention relates to recombinant furin (rFurin) and methods for producing rFurin. More specifically, the invention relates to substantially animal protein-free rFurin and methods for producing substantially animal protein-free rFurin.
摘要翻译： 本发明涉及重组弗林蛋白酶（rFurin）和生产rFurin的方法。 更具体地，本发明涉及基本上无动物蛋白的rFurin和用于生产基本上无动物蛋白的rFurin的方法。
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8.发明公开FORMULATION OF SUGAR SOLUTIONS FOR CONTINUOUS ULTRACENTRIFUGATION FOR VIRUS PURIFICATION 有权糖溶液进行不断的超速离心进行病毒清洁制剂
公开(公告)号：EP2155864A2
公开(公告)日：2010-02-24
申请号：EP08749278.1
申请日：2008-04-30
发明人： REITER, Manfred , GRILLBERGER, Leopold , MUNDT, Wolfgang , MITTERER, Artur , SCHAFHAUSER, Horst
CPC分类号： C12N7/00 , A61K39/00 , A61K39/12 , A61K39/145 , C12N2750/14351 , C12N2760/16134 , C12N2760/16151 , C12N2760/16152 , C12N2760/16234 , C12N2760/16251 , C12N2760/16252
摘要： The present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by the addition of two or more buffered sugar layers of different concentration. The method leads to higher yields and reduces unwanted aggregation of the virus or virus antigen by increasing the volume of the peak pool.
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9.发明公开
公开(公告)号：EP1453538A2
公开(公告)日：2004-09-08
申请号：EP02792955.3
申请日：2002-12-10
发明人： TAUER, Christa , MEYER, Heidi , MITTERER, Artur , BARRETT, Noel
CPC分类号： C12N7/00 , A61K39/12 , A61K39/29 , A61K2039/5252 , A61K2039/55505 , A61K2039/57 , C12N2770/32432 , C12N2770/32451 , Y02A50/464 , Y10S435/80 , Y10S435/803 , Y10S435/814 , Y10S435/816 , Y10S435/962
摘要： The present invention provides methods of purification of Hepatitis A Virus from the supernatant of an infected cell culture and production of a preparation of purified HAV antigen. The present invention is also directed to an HAV vaccine composition comprising a preparation consisting of purified mature HAV particles in an amount sufficient to induce a protective immune response in a mammal.
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10.发明公开METHOD OF ISOLATION AND PURIFICATION OF TRYPSIN FROM PRONASE AND USE THEREOF 有权方法分离和清洗过的胰蛋白酶链霉蛋白酶及其用途
公开(公告)号：EP1456228A2
公开(公告)日：2004-09-15
申请号：EP02798324.6
申请日：2002-12-10
IPC分类号： C07K1/22
CPC分类号： C12N9/6427 , C07K14/36 , C12N7/00 , C12N2760/16051 , C12N2760/16151 , C12N2770/32451
摘要： The present invention provides methods of isolation and purification of Streptomyces griseus trypsin (SGT) from pronase in a single affinity chromatography step and uses of the purified SGT.
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