公开(公告)号：EP1668118A2

公开(公告)日：2006-06-14

申请号：EP04783636.6

申请日：2004-09-10

申请人： Becton, Dickinson and Company

发明人： GUARINO, Richard, David ,  PRESNELL, Sharon, C. ,  LIEBMANN-VINSON, Andrea ,  HEIDARAN, Mohammad, A. ,  HEMPERLY, John, J. ,  JURGENSEN, Stewart ,  LAETHEM, Ronald, M. ,  ROBBINS, Neil

IPC分类号： C12N5/06 ,  C12N5/08 ,  G01N33/50

CPC分类号： G01N33/5067 ,  C12N5/067 ,  C12N5/0671 ,  C12N2533/32 ,  C12N2533/54

摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, an ECM protein, or a biologically active fragment or variant thereof such as elastin, fibronectin, vitronectin, laminin, collagen I, collagen III, collagen IV, and collagen VI. Also, optionally present on the surface is an active factor, preferably a polycationic polymer or a biologically active fragment or variant thereof, such as polyethyleneimine (PEI), poly-D-lysine (PDL), poly-L-lysine (PLL), poly-D-ornithine (PDO) or poly-L-ornithine (PLO). This surface is used in cell culture to promote cell attachment, survival, maintenance of function, and/or proliferation of primary liver cells. The invention also relates to methods utilizing this surface, such as methods for attachment, survival, maintenance of function, and/or proliferation of cells. Further disclosed is the use of the surface in cell culture with serum-free medium. Methods of screening using the surface of the invention are also disclosed.

公开(公告)号：EP1448053A2

公开(公告)日：2004-08-25

申请号：EP02800398.6

申请日：2002-09-30

申请人： Becton, Dickinson and Company

发明人： HEMPERLY, John, J.

IPC分类号： A01N1/02

CPC分类号： G01N33/5073 ,  C12N5/0623 ,  C12N2503/02 ,  C12N2506/11 ,  C12N2533/32 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/90 ,  G01N33/5008 ,  G01N33/5017 ,  G01N33/5029 ,  G01N33/5058

摘要： Methods and compositions for testing agents for their effects on growth and differentiation of cells, primarily stem cells of various origin, are disclosed. Also disclosed are methods for inducing growth and differentiation of bone marrow stem cells primarily along the pathway to neuronal progenitor cells.

公开(公告)号：EP1444325A1

公开(公告)日：2004-08-11

申请号：EP02803591.3

申请日：2002-09-30

申请人： Becton, Dickinson and Company

发明人： HEIDARAN, Mohammad, A. ,  MEYER, Mary, K. ,  ROWLEY, John, A. ,  HEMPERLY, John, J.

IPC分类号： C12M1/22

CPC分类号： C12N5/0654 ,  C12M25/14 ,  C12M41/46 ,  C12N2533/32 ,  C12N2533/40 ,  C12N2533/74

摘要： The present invention is directed to methods and devices which can be used to test bioactive agents alone or in conjunction with 3D scaffolds for their effect on cell growth, differentiation, and other cellular functions.