公开(公告)号：EP0724643A1

公开(公告)日：1996-08-07

申请号：EP94928873.0

申请日：1994-10-07

申请人： BOEHRINGER INGELHEIM INTERNATIONAL GmbH

发明人： COTTEN, Matthew ,  CHIOCCA, Susanna ,  SCHAFFNER, Gotthold ,  WAGNER, Ernst

IPC分类号： C12N15 ,  A61K47 ,  C12N1 ,  C12N7 ,  C12R1

CPC分类号： C12N15/87 ,  A61K47/6901

摘要： An endosomolytically active virus-like particle composed of units of capside proteins of viruses or virus-like particles, in which the capside protein units have a membrane-active peptidic sequence. These particles are suitable as a component of preparations for the transport of nucleic acids into higher eucaryotic cells via receptor-mediated endocytoses.

摘要翻译： 由病毒的帽面蛋白单元或病毒样颗粒构成的内切解活性病毒样颗粒，其中帽侧蛋白单元具有膜活性肽序列。 这些颗粒适合作为通过受体介导的内吞将核酸运输到高等真核细胞中的制剂组分。

公开(公告)号：EP0724643B1

公开(公告)日：2004-06-30

申请号：EP94928873.2

申请日：1994-10-07

申请人： Boehringer Ingelheim International GmbH

发明人： COTTEN, Matthew ,  CHIOCCA, Susanna ,  SCHAFFNER, Gotthold ,  WAGNER, Ernst

IPC分类号： C12N15/87 ,  C12N7/04 ,  C12N15/62

CPC分类号： C12N15/87 ,  A61K47/6901

摘要： An endosomolytically active virus-like particle composed of units of capside proteins of viruses or virus-like particles, in which the capside protein units have a membrane-active peptidic sequence. These particles are suitable as a component of preparations for the transport of nucleic acids into higher eucaryotic cells via receptor-mediated endocytoses.

公开(公告)号：EP0904394A1

公开(公告)日：1999-03-31

申请号：EP97919383.0

申请日：1997-04-18

申请人： Boehringer Ingelheim International GmbH

发明人： BAKER, Adam ,  COTTEN, Matthew ,  CHIOCCA, Susanna ,  KURZBAUER, Robert ,  SCHAFFNER, Gotthold

IPC分类号： C12N15 ,  A61K31 ,  A61K39 ,  A61K48 ,  A61P31 ,  A61P35 ,  C07K14 ,  C12N5 ,  C12N7 ,  C12R1

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K2039/525 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/10222 ,  C12N2710/10243

摘要： The invention relates to a chicken embryo lethal orphan (CELO) virus obtained by in vitro manipulation of plasmid-cloned CELO virus DNA. Said virus is suitable for production of vectors for gene therapy and as a vaccine against infectious diseases in humans and animals, in particular birds.

公开(公告)号：EP0767840A2

公开(公告)日：1997-04-16

申请号：EP95920887.0

申请日：1995-05-26

申请人： BOEHRINGER INGELHEIM INTERNATIONAL GmbH

发明人： COTTEN, Matthew ,  BAKER, Adam ,  CHIOCCA, Susanna

IPC分类号： C12N15 ,  A61K31 ,  A61K35 ,  A61K48 ,  A61P43 ,  C07K14 ,  C12N5

CPC分类号： C07K14/005 ,  C07K14/4702 ,  C07K14/4747 ,  C07K14/5412 ,  C07K14/5421 ,  C12N15/79 ,  C12N15/87 ,  C12N2710/10222 ,  C12N2710/10322

摘要： The toxicity problems arising when foreign matter is introduced into higher eukaryotic cells, especially with transfection with DNA, are obviated by expression in the cell of gene products that block the apoptosis induced by the transfection process and/or by treating the cells with anti-inflammatory substances. Preferred anti-apoptosis genes are Bcl-2, E1B 19K or an anti-apoptotic gene of the CELO avian adenovirus; used as preferred anti-inflammatory substance in the adenovirus VA1, which is introduced into the cell in the form of VA1 DNA. These measures help to achieve a long-lasting gene expression.