摘要:
An improved determination of peroxidase activity by end dilution titration is carried out with a benzidine derivative of the general formula I where
R1 stands for a hydrogen atom, a halogen atom, an amino group, a (C 1 -C 4 ) alkyl group, a (C 1 -C 4 ) alkoxy group, a carboxy group, a sulfo group or a carboxypropyloxy group and where R2 stands for a hydrogen atom, a halogen atom, a (C 1 -C 4 ) alkyl group, or a salt of this benzidine compound, in the presence of a non-chromogenic or low-chromogenic phenylene derivative of the general formula II
where R3 and R4 are equal or different and stand for a hydroxy group or an amino group, a (C 1 -C 4 ) alkyl amino group or a benzylamino group.
摘要:
A procedure for the immobilization of proteins on plystyrene surfaces which includes a pre-treatment of the polystyrene surface with a bis-diazonium compound of the general formula I where
R1 stands for a hydrogen atom, a halogen atom, an alkyl group, an alkoxy group or a nitro group and where R2 stands for a hydrogen atom, a halogen atom or an alkyl group and where X stands for an anion and the subsequent adsorption of the protein on the surface pretreated. Proteins immobilized by this procedure may be used in immunoassays.