公开(公告)号：EP2299256A3

公开(公告)日：2012-10-10

申请号：EP10178333.0

申请日：2001-09-14

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

发明人： Quake, Stephen R. ,  Thorsen, Todd

IPC分类号： G01N15/14

CPC分类号： C12Q1/68 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2200/12 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0454 ,  B01L2400/0481 ,  B01L2400/0484 ,  B01L2400/0487 ,  B01L2400/0638 ,  C12M1/34 ,  G01N15/1404 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N33/54366 ,  G01N35/08 ,  G01N2015/0065 ,  G01N2015/0088 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/1411 ,  G01N2015/1481 ,  G01N2015/149 ,  Y10T436/118339 ,  Y10T436/143333

摘要： A microfluidic device for analyzing and/or sorting biological material (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix. Biological material within the droplets can be analyzed and/or sorted by detecting a predetermined characteristic of the biological sample in each droplet and sorting the droplet accordingly.

公开(公告)号：EP2299256A2

公开(公告)日：2011-03-23

申请号：EP10178333.0

申请日：2001-09-14

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

发明人： Quake, Stephen R. ,  Thorsen, Todd

IPC分类号： G01N15/14

CPC分类号： C12Q1/68 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2200/12 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0454 ,  B01L2400/0481 ,  B01L2400/0484 ,  B01L2400/0487 ,  B01L2400/0638 ,  C12M1/34 ,  G01N15/1404 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N33/54366 ,  G01N35/08 ,  G01N2015/0065 ,  G01N2015/0088 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/1411 ,  G01N2015/1481 ,  G01N2015/149 ,  Y10T436/118339 ,  Y10T436/143333

摘要： A microfluidic device for analyzing and/or sorting biological material (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix. Biological material within the droplets can be analyzed and/or sorted by detecting a predetermined characteristic of the biological sample in each droplet and sorting the droplet accordingly.

摘要翻译： 提供了用于分析和/或分选生物材料的微流体装置（例如分子，例如多核苷酸和多肽，包括蛋白质和酶;病毒和细胞）及其使用方法。 本发明的装置和方法可用于分选颗粒，例如 病毒颗粒。 本发明也可用于高通量筛选，例如 组合筛选。 微流体装置包括在液滴挤出区域处与主通道连通的主通道和入口区域。 含有生物材料的溶液的液滴通过液滴挤出区沉积到主通道中。 与包含生物材料的溶液不同且不相容的流体流过主通道，使得含有生物材料的液滴不会扩散或混合。 可以通过检测每个液滴中生物样品的预定特征并相应地分选液滴来分析和/或分选液滴内的生物材料。