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公开(公告)号：EP1847605A3
公开(公告)日：2007-10-31
申请号：EP07006223.7
申请日：1991-07-10
发明人： McCafferty, John , Pope, Anthony Richard , Johnson, Kevin Stuart , Hoogenboom, Hendricus Renerus Jacobus Mattheus , Griffiths, Andrew David , Jackson, Ronald Henry , Holliger, Kaspar Philipp , Marks, James David , Clackson, Timothy Piers , Chiswell, David John , Winter, Gregory Paul , Bonnert, Timothy Peter
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： 特定结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的基因多样性群体的DNA来鉴定，其中sbp成员以功能形式显示在分泌的重组遗传展示包（rgdp ）含有编码sbp成员的DNA或其多肽组分，因为sbp成员或其多肽组分与rgdp的衣壳组分表达融合。 显示的sbps可以通过与互补sbp成员的亲和力来选择，并且从选择的rgdps恢复的DNA用于表达选定的sbp成员。 因此可以获得抗体sbp成员，其不同的链表达，一个融合至衣壳组分，另一个以游离形式与融合伴侣多肽结合。 噬菌粒可用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以将编码这种多聚体sbp成员各自链的DNA文库组合起来，从而在sbp成员中获得比通过常规方法容易获得的更大的遗传多样性。
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2.发明公开Methods for producing members of specific binding pairs 失效Verfahren zur Herstellung von spezifischen Bindungspaargliedern
公开(公告)号：EP1847605A2
公开(公告)日：2007-10-24
申请号：EP07006223.7
申请日：1991-07-10
发明人： McCafferty, John , Pope, Anthony Richard , Johnson, Kevin Stuart , Hoogenboom, Hendricus Renerus Jacobus Mattheus , Griffiths, Andrew David , Jackson, Ronald Henry , Holliger, Kaspar Philipp , Marks, James David , Clackson, Timothy Piers , Chiswell, David John , Winter, Gregory Paul , Bonnert, Timothy Peter
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这样的sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以功能形式在分泌的重组遗传显示包（rgdp）的表面显示 ），其含有编码sbp成员或其多肽组分的DNA，由于sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与互补sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而在sbp成员中获得比通过常规方法容易获得的更大的遗传多样性。
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公开(公告)号：EP1754787A2
公开(公告)日：2007-02-21
申请号：EP06018386.0
申请日：1991-07-10
发明人： McCafferty, John , Pope, Anthony Richard , Johnson, Kevin Stuart , Hoogenboom, Hendricus Renerus Jacobus Mattheus , Griffiths, Andrew David , Jackson, Ronald Henry , Holliger, Kaspar Philipp , Marks, James David , Clackson, Timothy Piers , Chiswell, David John , Winter, Gregory Paul , Bonnert, Timothy Peter
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
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公开(公告)号：EP0844306B1
公开(公告)日：2006-09-13
申请号：EP97120149.6
申请日：1991-07-10
发明人： McCafferty, John , Pope, Anthony Richard , Johnson, Kevin Stuart , Hoogenboom, Hendricus Renerus Jacobus Mattheus , Griffiths, Andrew David , Jackson, Ronald Henry , Holliger, Kaspar Philipp , Marks, James David , Clackson, Timothy Piers , Chiswell, David John , Winter, Gregory, Paul , Bonnert, Timothy Peter
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
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5.发明公开Methods of producing members of specific binding pairs 失效孙中山，古董店| |用于生产长丝的方法RPER通过使用噬菌体和噬菌体Daduchus hergestelten呈现噬菌体颗粒
公开(公告)号：EP0774511A1
公开(公告)日：1997-05-21
申请号：EP96112510.1
申请日：1991-07-10
发明人： McCafferty, John , Pope, Anthony Richard , Johnson, Kevin Stuart , Hoogenboom, Hendricus Renerus Jacobus Mattheus , Griffiths, Andrew David , Jackson, Ronald Henry , Holliger, Kaspar Philipp , Marks, James David , Clackson, Timothy Piers , Chiswell, David John , Winter, Gregory Paul , Bonnert, Timothy Peter
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： 特异性结合对（SBP）的成员被识别通过表达编码寻求在重组宿主细胞中的sbp成员的遗传多样性群体，其中SBP部件在分泌的重组遗传展示包装的表面（RGDP显示功能形式的DNA ）编码伴侣成员或其多肽组分包含DNA，借助于伴侣成员或其被过表达与RGDP的衣壳组分的融合的多肽组分。 所显示的SBPS可以通过亲和与互补的SBP构件被选择，该DNA选自rgdps回收所选伴侣成员的表达。 抗体的特异性结合伴侣构件可以如此获得，具有不同的链上过表达的，一个稠合到衣壳组分和其他游离形式用于与融合配偶体多肽关联。 噬菌粒可被用作在表达载体中，与所述衣壳融合帮助包装噬菌粒DNA。 使用多聚体寻求SBP成员的DNA编码respectivement链的这种方法的库可以被组合，从而获得在SBP成员比可以很容易地通过常规方法获得更大的遗传多样性。
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公开(公告)号：EP1754787A3
公开(公告)日：2008-06-18
申请号：EP06018386.0
申请日：1991-07-10
发明人： McCafferty, John , Pope, Anthony Richard , Johnson, Kevin Stuart , Hoogenboom, Hendricus Renerus Jacobus Mattheus , Griffiths, Andrew David , Jackson, Ronald Henry , Holliger, Kaspar Philipp , Marks, James David , Clackson, Timothy Piers , Chiswell, David John , Winter, Gregory Paul , Bonnert, Timothy Peter
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
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7.发明授权Phagemid-based method of producing filamentous bacteriophage particles displaying antibody molecules and the corresponding bacteriophage particles 失效用于通过使用噬菌粒的生产丝状噬菌体抗体呈递的和产生Daduchus噬菌体方法
公开(公告)号：EP0774511B1
公开(公告)日：2002-01-30
申请号：EP96112510.1
申请日：1991-07-10
发明人： McCafferty, John , Pope, Anthony Richard , Johnson, Kevin Stuart , Hoogenboom, Hendricus Renerus Jacobus Mattheus , Griffiths, Andrew David , Jackson, Ronald Henry , Holliger, Kaspar Philipp , Marks, James David , Clackson, Timothy Piers , Chiswell, David John , Winter, Gregory Paul , Bonnert, Timothy Peter
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
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8.发明公开Methods of producing members of specific binding pairs 失效Verfahren zur Herstellung von spezifischen Bindungspaargliedern
公开(公告)号：EP0844306A1
公开(公告)日：1998-05-27
申请号：EP97120149.6
申请日：1991-07-10
发明人： McCafferty, John , Pope, Anthony Richard , Johnson, Kevin Stuart , Hoogenboom, Hendricus Renerus Jacobus Mattheus , Griffiths, Andrew David , Jackson, Ronald Henry , Holliger, Kaspar Philipp , Marks, James David , Clackson, Timothy Piers , Chiswell, David John , Winter, Gregory, Paul , Bonnert, Timothy Peter
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这样的sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以功能形式在分泌的重组遗传显示包（rgdp）的表面显示 ），其含有编码sbp成员或其多肽组分的DNA，由于sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与互补sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而在sbp成员中获得比通过常规方法容易获得的更大的遗传多样性。
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9.发明授权Specific binding members for human transforming growth factor beta; materials and methods 失效人类TGF-β的材料和方法特异性结合的合作伙伴
公开(公告)号：EP0945464B1
公开(公告)日：2001-02-07
申请号：EP99102166.8
申请日：1996-10-07
发明人： Thompson, Julia Elizabeth , Williams, Andrew James , Vaughan, Tristan John , Green, Jonathan Alexander , Johnson, Kevin Stuart , Tempest, Philip Ronald , Wilton, Alison Jane
IPC分类号： C07K16/22
CPC分类号： C07K16/22 , A61K38/00 , C07K2317/21 , C07K2317/622
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10.发明公开Specific binding members for human transforming growth factor beta; materials and methods 失效人类TGF-β的材料和方法特异性结合的合作伙伴
公开(公告)号：EP0945464A1
公开(公告)日：1999-09-29
申请号：EP99102166.8
申请日：1996-10-07
发明人： Thompson, Julia Elizabeth , Williams, Andrew James , Vaughan, Tristan John , Green, Jonathan Alexander , Johnson, Kevin Stuart , Tempest, Philip Ronald , Wilton, Alison Jane
IPC分类号： C07K16/22
CPC分类号： C07K16/22 , A61K38/00 , C07K2317/21 , C07K2317/622
摘要： Specific binding members comprising human antibody antigen binding domains specific for human transforming growth factor beta (TGFβ) bind specifically isoforms TGFβ2 and TGFβ1 or both, preferentially compared with TGFβ3. Specific binding members may be isolated and utilised in the treatment of disease, particularly fibrotic disease and also immune/inflammatory diseases. Therapeutic utility is demonstrated using in vitro and in vivo models. Full sequence and binding information is provided, including epitope sequence information for a particularly advantageous specific binding member which binds the active form of TGFβ2, neutralising its activity, but does not bind the latent form.
摘要翻译： 包含人抗体的抗原结合特异性的人转化生长因子β（TGF测试版）特异性结合亚型TGF的β2和TGF的β1或两个结构域特异性结合成员，优先与TGF测试相比3.特异性结合成员可以在被分离和利用 治疗疾病，特别是纤维化疾病并因此免疫/炎性疾病。 治疗效用，使用体外和体内模型中以实例阐述。 全序列，并且设置bindingInformation，包括其结合TGFβ2受体，中性伊辛其活性的活性形式，但不结合的潜在形式的特别有利的特异性结合成员的表位的序列信息。
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