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    PROCEDE DE NORMALISATION DE LA LUMINESCENCE EMISE PAR UN MILIEU DE MESURE.
    9.
    发明公开
    PROCEDE DE NORMALISATION DE LA LUMINESCENCE EMISE PAR UN MILIEU DE MESURE. 审中-公开
    进行归一化方法通过测量介质所发射的光

    公开(公告)号：EP2817614A1

    公开(公告)日：2014-12-31

    申请号：EP12710984.1

    申请日：2012-02-22

    申请人： Cisbio Bioassays

    发明人： MATHIS, Gérard ,  BAZIN, Hervé

    IPC分类号： G01N21/64 ,  G01N33/58 ,  G01N33/542

    CPC分类号： G01N21/6428 ,  G01N21/274 ,  G01N21/64 ,  G01N21/6408 ,  G01N33/542 ,  G01N33/58 ,  G01N33/582 ,  G01N2021/6413 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/06113 ,  G06F19/10

    摘要： The subject matter of the invention is a method for measuring the luminescence of a long-life fluorescent compound present in a measuring medium, said medium containing a biological sample, characterised in that it comprises the following steps: a) introduction of a long-life fluorescent compound into the measuring medium, b) introduction, into the measuring medium, of a fluorescent marking agent, the absorption spectrum of which allows the excitation thereof at the same wavelength as that used to excite the long-life fluorescent compound, wherein said emission spectrum allows the measurement of the luminescence thereof at the same wavelength as that used to measure the luminescence of the long-life fluorescent compound, c) excitation of the measuring medium with a wavelength corresponding to an absorption peak of the long-life fluorescent compound, d) measurement of the luminescence emitted by the measuring medium immediately after excitation of said medium, mainly corresponding to the luminescence of the marking agent, and for a period of 5 ns to 45 ps, at a wavelength corresponding to an emission peak of the long-life fluorescent compound, e) time-resolved measurement of the luminescence emitted by the measuring medium at the same wavelength as that used in step d), after a delay of 20 to 200 ps following the excitation of the measuring medium and for a period of 200 to 1000 ps, said luminescence mainly corresponding to that of the long-life fluorescent compound, f) calculation of a normalised luminescence signal corresponding to the ratio: (signal obtained in step e) / (signal obtained in step d).