利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

10 条记录 (耗时 0.037 秒)

    PROCEDE DE NORMALISATION DE LA LUMINESCENCE EMISE PAR UN MILIEU DE MESURE.
    3.
    发明公开
    PROCEDE DE NORMALISATION DE LA LUMINESCENCE EMISE PAR UN MILIEU DE MESURE. 审中-公开
    进行归一化方法通过测量介质所发射的光

    公开(公告)号：EP2817614A1

    公开(公告)日：2014-12-31

    申请号：EP12710984.1

    申请日：2012-02-22

    申请人： Cisbio Bioassays

    发明人： MATHIS, Gérard ,  BAZIN, Hervé

    IPC分类号： G01N21/64 ,  G01N33/58 ,  G01N33/542

    CPC分类号： G01N21/6428 ,  G01N21/274 ,  G01N21/64 ,  G01N21/6408 ,  G01N33/542 ,  G01N33/58 ,  G01N33/582 ,  G01N2021/6413 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/06113 ,  G06F19/10

    摘要： The subject matter of the invention is a method for measuring the luminescence of a long-life fluorescent compound present in a measuring medium, said medium containing a biological sample, characterised in that it comprises the following steps: a) introduction of a long-life fluorescent compound into the measuring medium, b) introduction, into the measuring medium, of a fluorescent marking agent, the absorption spectrum of which allows the excitation thereof at the same wavelength as that used to excite the long-life fluorescent compound, wherein said emission spectrum allows the measurement of the luminescence thereof at the same wavelength as that used to measure the luminescence of the long-life fluorescent compound, c) excitation of the measuring medium with a wavelength corresponding to an absorption peak of the long-life fluorescent compound, d) measurement of the luminescence emitted by the measuring medium immediately after excitation of said medium, mainly corresponding to the luminescence of the marking agent, and for a period of 5 ns to 45 ps, at a wavelength corresponding to an emission peak of the long-life fluorescent compound, e) time-resolved measurement of the luminescence emitted by the measuring medium at the same wavelength as that used in step d), after a delay of 20 to 200 ps following the excitation of the measuring medium and for a period of 200 to 1000 ps, said luminescence mainly corresponding to that of the long-life fluorescent compound, f) calculation of a normalised luminescence signal corresponding to the ratio: (signal obtained in step e) / (signal obtained in step d).

    METHODE THERANOSTIQUE BASEE SUR LA DETECTION DE DIMERES HER2-HER2
    10.
    发明公开
    METHODE THERANOSTIQUE BASEE SUR LA DETECTION DE DIMERES HER2-HER2 有权
    通过THERANOSTIQUE BASEE SUR LA检测HER2-HER2

    公开(公告)号：EP2875357A1

    公开(公告)日：2015-05-27

    申请号：EP13744749.6

    申请日：2013-07-16

    申请人： Cisbio Bioassays ,  Institut Gustave Roussy

    发明人： ANDRE, Fabrice ,  BAZIN, Hervé ,  GARNERO, Patrick ,  MATHIS, Gérard

    IPC分类号： G01N33/574 ,  G01N33/74

    CPC分类号： G01N33/57492 ,  G01N33/57415 ,  G01N33/74 ,  G01N2333/71

    摘要： The present invention relates to an
    ex vivo method for determining the susceptibility of a patient suffering from cancer to respond to a therapeutic treatment based on the administration of an antibody specific for the HER2 protein, this method comprising the quantification of HER2-HER2 dimers in a tissue sample from said patient using the FRET technique. The invention also relates to a kit of reagents for implementing this method.

    摘要翻译： 本发明涉及用于测定罹患癌症的患者对基于施用HER2蛋白特异性抗体的治疗性治疗的敏感性的离体方法，该方法包括定量测定HER2-HER2二聚体 使用FRET技术从所述患者组织样品。 本发明还涉及用于实施该方法的试剂盒。