公开(公告)号：EP1960516B1

公开(公告)日：2012-12-26

申请号：EP06850558.5

申请日：2006-11-17

申请人： Cargill, Incorporated

发明人： MILLER, Matthew ,  SUOMINEN, Pirkko ,  ARISTIDOU, Aristos ,  HAUSE, Benjamin, Matthew ,  VAN HOEK, Pim ,  DUNDON, Catherine, Asleson

IPC分类号： C12N9/04 ,  C12N1/20 ,  C12N15/00 ,  C12P7/56 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12P7/56 ,  C12Y101/02003

公开(公告)号：EP2001992A2

公开(公告)日：2008-12-17

申请号：EP07753061.6

申请日：2007-03-13

申请人： Cargill, Incorporated

发明人： DUNDON, Catherine, Asleson ,  SUOMINEN, Pirkko ,  ARISTIDOU, Aristos ,  RUSH, Brian, J. ,  KOIVURANTA, Kari ,  HAUSE, Benjamin, Matthew ,  MCMULLIN, Thomas, William ,  ROBERG-PEREZ, Kevin

IPC分类号： C12N1/18

CPC分类号： C12N15/52 ,  C12P7/06 ,  C12P7/40 ,  C12P7/56 ,  Y02E50/17

摘要： Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the genera Kluyυeromyces, Candida or Issatchenkia. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.

摘要翻译： 在发酵过程中，在发酵条件下和在碳源的存在下培养经遗传修饰以将天然代谢途径从磷酸二羟丙酮磷酸酯切割至甘油的全基因组复制酵母的细胞，以产生 所需的发酵产物，其中甘油产率基于由细胞消耗的碳源的重量小于2％，并且其中将甘油加入到发酵培养基中。