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公开(公告)号：EP3871773A1
公开(公告)日：2021-09-01
申请号：EP20159936.2
申请日：2020-02-27
申请人： Cellix Limited
摘要： A method of determining the transfection status of a plurality of cells comprising the steps of providing a population of cells that have been subjected to a cell membrane disruption treatment, passing a focussed stream of the population of cells in a carrier liquid along a microfluidic channel having a detection zone comprising a pair of detection electrodes configured to detect electrical impedance across the channel in the detection zone, detecting a change in the electrical impedance across the channel in the detection zone corresponding to each of the plurality of cells passing the detection zone, comparing the change in electrical impedance for each cell with a reference change in electrical impedance, and calculating the transfection status of the plurality of cells based on the comparison.
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2.发明公开
公开(公告)号：EP2514528A1
公开(公告)日：2012-10-24
申请号：EP12164728.3
申请日：2012-04-19
申请人： Cellix Limited
CPC分类号： B01L3/5027 , B01L3/502715 , B01L3/50825 , B01L2200/027 , B01L2200/0689 , B01L2300/0636 , B01L2300/0645 , B01L2300/0672 , B01L2300/0816 , B01L2300/0883 , B01L2400/0478 , B01L2400/0683 , B01L2400/086
摘要： A device for assessing the status of a fluid containing cells of mammalian origin, the device comprises a cartridge (2) having an enclosed fluidic channel (28), an assay area (22) disposed within the fluidic channel (28) which is covered by at least one agent capable of selectively recruiting at least one type of cell contained within the mammalian fluid, an inlet port (25) for the fluidic channel, an outlet port (24) for the fluidic channel and a piercing means (51) adapted for connecting to a sealed container (4) containing the cell containing fluid by puncturing the container. The inlet port and piercing means are adapted to provide fluid communication between the fluidic channel and the sealed container when the sealed contained is punctured by the piercing means. The outlet port is adapted for fluid communication with a syringe (3) such that actuation of the syringe provides controlled flow of fluid over the assay area to achieve desired physiological shear stress conditions for the type of cell being recruited at the assay area. The cartridge is adapted for integration with a read out instrument for acquiring optical signal at the assay area of the channel.
摘要翻译： 一种用于assesing哺乳动物来源的流体含有细胞的状态的装置，该装置包括流体通道（28）内设置的盒（2），其具有在封闭流体通道（28）以测定区域（22），所有这些是由覆盖 能够选择性地招募包含在哺乳动物流体中的至少一种类型的细胞，入口（25）的端口，用于在出口（24）的流体通道的流体通道和一个刺穿装置（51）用于angepasst的至少一个代理 连接到密封容器（4）含有通过刺穿容器含有流体的单元格。 入口端口和刺穿装置被angepasst以提供流体通道和所述密封的容器当被密封包含由刺穿装置刺穿之间的流体连通。 出口端口是angepasst用于注射器的流体连通（3）搜索做的注射器的致动提供控制在化验区，以实现在测定区域被招募用于细胞的类型的期望生理剪切应力条件的流体的流动。 盒被angepasst集成有用于在信道的所述测定区域获取的光信号读出仪。
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公开(公告)号：EP3418717A1
公开(公告)日：2018-12-26
申请号：EP17177624.8
申请日：2017-06-23
申请人： Cellix Limited
发明人： Kashanin, Dmitry , Shvets, Igor , Mc Auley, Robert
CPC分类号： G01N15/10 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L2200/0652 , B01L2300/0627 , B01L2300/0645 , B01L2300/0864 , B01L2400/0415 , C12N5/0612 , G01N15/1056 , G01N15/1459 , G01N15/1484 , G01N2015/1006 , G01N2015/1081
摘要： A microfluidic apparatus for separation of particulates in a fluidAn apparatus for separation of particulates in a fluid (3A) into subsets of particulates comprises a microfluidic chip (1) comprising: a microfluidic channel having a fluid inlet for receipt of a stream of particulate containing fluid (3A), a detection zone disposed in the microfluidic channel and comprising a sensor configured to detect changes in the microfluidic channel corresponding to particulates passing the sensor, and a separation zone distal of the detection zone in which the microfluidic channel divides into at least two secondary microfluidic channels (4). The separation zone comprises two or more separation electrodes (16A, 16B) including at least one separation electrode (16A, 16B, 16C, 16D) disposed in electrical contact with an interior of the microfluidic channel and at least one further separation electrode (16A, 16B, 16C, 16D) disposed in electrical contact with an interior of the microfluidic channel or one of the secondary microfluidic channels (4). The pair of separation electrodes (16A, 16B) are configured to pass a pulse of electrical current through the separation zone of the microfluidic channel.
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公开(公告)号：EP2623587A1
公开(公告)日：2013-08-07
申请号：EP12153868.0
申请日：2012-02-03
申请人： Cellix Limited
发明人： Shvets, Igor , Kashanin, Dmitry , Dohen, Christophe , Paul, Toby
IPC分类号： C12M3/00
摘要： An apparatus (1) for culturing live cells comprising a cell culture vessel (5), a fresh culture medium storage vessel (3), a used culture medium collection vessel (4), a supply conduit (9, 10) adapted to provide fluid communication between the fresh culture medium storage vessel and a first end of the cell culture vessel, an inlet of the supply conduit being located towards a base of the fresh culture medium storage vessel, a drainage conduit (12) adapted to provide fluid communication between a second end of the cell culture vessel and the used culture medium collection vessel, an inlet of the drainage conduit being disposed within the cell culture vessel such that in use it is it located in the culture medium, and a pump (2) adapted to pump fresh culture medium from the fresh culture medium storage vessel (3) to one end of the cell culture vessel (5) and pump used culture medium from the second end of the cell culture vessel to the used culture medium collection vessel (4). The cell culture vessel (5) is generally not sealed to ambient, and the apparatus includes a pressure equalization conduit (11) adapted to provide fluid communication between a headspace of the used culture medium collection vessel (4) and a headspace of the fresh culture medium storage vessel (3).
摘要翻译： 用于培养适于提供流体活细胞，其包括细胞培养容器（5），一个新鲜的培养介质存储容器（3），使用的培养基的收集容器（4），供给管道（9，10）的装置（1） 新鲜的培养液贮藏容器和细胞培养容器中的供给导管的入口的第一端之间的通信被定位为朝向新鲜培养基储存容器的一基座，一排水管路（12）angepasst提供一种之间的流体连通 细胞培养容器中，并在排水导管的入口处的使用培养基收集容器的第二端部的细胞培养容器的搜索结果在使用这样做是被它位于在培养基内设置，和一个泵（2）angepasst泵 从新鲜培养基储存容器（3）向细胞培养容器的一端新鲜培养基（5）并从细胞培养容器的第二端部到培养基中用于收集容器泵中使用的培养基（4）。 细胞培养容器（5）是不密封到环境基因反弹，并且该装置包括一个压力平衡管道（11）angepasst所使用的培养基的收集容器的顶部空间之间提供流体连通，（4）和新鲜培养物的顶部空间 介质存储容器（3）。
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公开(公告)号：EP2623587B1
公开(公告)日：2019-11-20
申请号：EP12153868.0
申请日：2012-02-03
申请人： Cellix Limited
发明人： Shvets, Igor , Kashanin, Dmitry , Dohen, Christophe , Paul, Toby
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公开(公告)号：EP3418719A1
公开(公告)日：2018-12-26
申请号：EP17177662.8
申请日：2017-06-23
申请人： Cellix Limited
发明人： Kashanin, Dmitry , Shvets, Igor
IPC分类号： G01N15/10
CPC分类号： G01N15/1056 , G01N15/1031 , G01N2015/1006 , G01N2015/1093
摘要： This invention relates to the field of microfluidic flow cytometry and more generally microfluidic techniques for analysis of particulate-containing fluids. It deals with the improvements to such technologies in order to identify subsets of particles or sub-populations of cells that differ in their properties, and, if necessary, separate the said identified sub-populations of cells, e.g. sex of semen cells, alive cells from the dead ones, cancerous cells from the healthy ones, subsets of viruses, bacteria or subsets of particles. This invention disclosure deals with the apparatus and the method for detection of cells or particles based on measurements of complex AC impedance between electrodes across the flow of fluid containing such cells or particles.
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