公开(公告)号：EP2398897B1

公开(公告)日：2017-06-28

申请号：EP10705729.1

申请日：2010-02-22

申请人： Cellular Dynamics International, Inc.

发明人： DAIGH, Christine, A. ,  FUHRKEN, Peter ,  SALVAGIOTTO, Giorgia

IPC分类号： C12N5/0735 ,  C12N5/0789

CPC分类号： C12N5/0606 ,  C12N5/0647 ,  C12N5/069 ,  C12N2500/02 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/70 ,  C12N2506/02

公开(公告)号：EP2398897A1

公开(公告)日：2011-12-28

申请号：EP10705729.1

申请日：2010-02-22

申请人： Cellular Dynamics International, Inc.

发明人： DAIGH, Christine, A. ,  FUHRKEN, Peter ,  SALVAGIOTTO, Giorgia

IPC分类号： C12N5/0735 ,  C12N5/0789

CPC分类号： C12N5/0606 ,  C12N5/0647 ,  C12N5/069 ,  C12N2500/02 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/70 ,  C12N2506/02

摘要： The present invention provides methods and compositions for the production of hematopoietic progenitor cells or endothelial progenitor cells from human pluripotent stem cells using a defined cell culture medium without the need to utilize feeder cells or serum. In some embodiments, differentiation is accomplished using hypoxic atmospheric conditions. The defined medium of the present invention may contain growth factors and a matrix component. The hematopoietic progenitor cells may be further differentiated into cell lineages including red blood cells, macrophages, granulocytes, and megakaryocytes. The endothelial progenitor cells may be further differentiated into endothelial cells. Also disclosed are screening assays for identification of candidate substances that affect differentiation of pluripotent stem cells into progenitor cells.

摘要翻译： 本发明提供了使用限定的细胞培养基从人多能干细胞生产造血祖细胞或内皮祖细胞而不需要利用饲养细胞或血清的方法和组合物。 在一些实施方案中，使用缺氧大气条件实现分化。 本发明的限定的培养基可以含有生长因子和基质成分。 造血祖细胞可以进一步分化成细胞系，包括红细胞，巨噬细胞，粒细胞和巨核细胞。 内皮祖细胞可进一步分化为内皮细胞。 还公开了用于鉴定影响多能干细胞分化为祖细胞的候选物质的筛选测定。