公开(公告)号：EP0880585B1

公开(公告)日：2006-05-24

申请号：EP97903440.2

申请日：1997-02-12

申请人： Cobra Biologics Limited

发明人： THATCHER, David, Robert ,  HITCHCOCK, Antony, Gordon ,  HANAK, Julian, Alexis ,  VARLEY, Diane, Lesley

IPC分类号： C12N15/10 ,  C07H1/08

CPC分类号： C12N1/06 ,  C12N15/101 ,  C12N15/70

摘要： A scalable method for the production of highly purified plasmid DNA in Escherichia coli is described, which method includes growing plasmid-containing cells to a high biomass in exponential growth and lysing the cells by raising the pH of the culture to a carefully controlled pH value in which chromosomal DNA is denatured but plasmid DNA is reversibly renatured. The method has been developed for the production of pharmaceutical grade DNA for use in in vivo and ex vivo gene therapy.