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公开(公告)号：EP3260544A1
公开(公告)日：2017-12-27
申请号：EP17182502.9
申请日：2009-11-17
申请人： Commonwealth Scientific and Industrial Research Organisation , Grains Research And Development Corporation
发明人： PETRIE, James, Robertson , MACKENZIE, Anne Maree , LIU, Qing , SHRESTHA, Pushkar , NICHOLS, Peter David , BLACKBURN, Susan, Irene, Ellis , MANSOUR, Maged, Peter , ROBERT, Stanley, Suresh , FRAMPTON, Dion, Matthew, Frederick , ZHOU, Xue-Rong , SINGH, Surinder, Pal , WOOD, Craig, Christopher
CPC分类号： C11B3/006 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N9/93 , C12N15/52 , C12N15/8202 , C12N15/8205 , C12N15/8216 , C12N15/8218 , C12N15/8234 , C12N15/8247 , C12N15/8257 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12Y114/00 , C12Y114/19 , C12Y114/19003 , C12Y602/01003
摘要： The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ω3 destaurases, Δ5 elongases and Δ6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.
摘要翻译： 本发明涉及在重组细胞如酵母或植物细胞中合成长链多不饱和脂肪酸，特别是二十碳五烯酸，二十二碳五烯酸和二十二碳六烯酸的方法。 还提供了产生长链多不饱和脂肪酸的重组细胞或植物。 此外，本发明涉及一组具有去饱和酶或延伸酶活性的新酶，其可用于合成长链多不饱和脂肪酸的方法中。 特别地，本发明提供具有新活性的ω3去饱和酶，Δ5延伸酶和Δ6去饱和酶。 还提供了用多种基因瞬时和/或稳定转化细胞，特别是植物细胞的方法和DNA构建体。
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公开(公告)号：EP2358882B1
公开(公告)日：2017-07-26
申请号：EP09827035.8
申请日：2009-11-17
申请人： Commonwealth Scientific and Industrial Research Organisation , Grains Research And Development Corporation
发明人： PETRIE, James, Robertson , MACKENZIE, Anne Maree , LIU, Qing , SHRESTHA, Pushkar , NICHOLS, Peter David , BLACKBURN, Susan, Irene, Ellis , MANSOUR, Maged, Peter , ROBERT, Stanley, Suresh , FRAMPTON, Dion, Matthew, Frederick , ZHOU, Xue-Rong , SINGH, Surinder, Pal , WOOD, Craig, Christopher
CPC分类号： C11B3/006 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N9/93 , C12N15/52 , C12N15/8202 , C12N15/8205 , C12N15/8216 , C12N15/8218 , C12N15/8234 , C12N15/8247 , C12N15/8257 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12Y114/00 , C12Y114/19 , C12Y114/19003 , C12Y602/01003
摘要： The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides É3 destaurases, ”5 elongases and ”6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.
摘要翻译： 本发明一般涉及重组脂肪酸合成领域，特别是在转基因植物中。 该申请描述了涉及脂肪酸合成的基因并提供了用于操作植物油的脂肪酸组成的方法和载体。 具体而言，本发明提供了用于实现将涉及脂肪酸合成的多个异源基因整合到植物基因组中的构建体，从而使得到的植物产生改变水平的多不饱和脂肪酸。 还描述了通过在植物贮藏器官内共表达沉默抑制子来增强脂肪酸生物合成酶的表达的方法。
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公开(公告)号：EP2358882A1
公开(公告)日：2011-08-24
申请号：EP09827035.8
申请日：2009-11-17
申请人： Commonwealth Scientific and Industrial Research Organisation , Grains Research And Development Corporation
发明人： PETRIE, James, Robertson , MACKENZIE, Anne Maree , LIU, Qing , SHRESTHA, Pushkar , NICHOLS, Peter David , BLACKBURN, Susan, Irene, Ellis , MANSOUR, Maged, Peter , ROBERT, Stanley, Suresh , FRAMPTON, Dion, Matthew, Frederick , ZHOU, Xue-Rong , SINGH, Surinder, Pal , WOOD, Craig, Christopher
CPC分类号： C11B3/006 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N9/93 , C12N15/52 , C12N15/8202 , C12N15/8205 , C12N15/8216 , C12N15/8218 , C12N15/8234 , C12N15/8247 , C12N15/8257 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12Y114/00 , C12Y114/19 , C12Y114/19003 , C12Y602/01003
摘要： The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides É3 destaurases, ”5 elongases and ”6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.
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