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公开(公告)号：EP3260544A1
公开(公告)日：2017-12-27
申请号：EP17182502.9
申请日：2009-11-17
Applicant: Commonwealth Scientific and Industrial Research Organisation , Grains Research And Development Corporation
Inventor： PETRIE, James, Robertson , MACKENZIE, Anne Maree , LIU, Qing , SHRESTHA, Pushkar , NICHOLS, Peter David , BLACKBURN, Susan, Irene, Ellis , MANSOUR, Maged, Peter , ROBERT, Stanley, Suresh , FRAMPTON, Dion, Matthew, Frederick , ZHOU, Xue-Rong , SINGH, Surinder, Pal , WOOD, Craig, Christopher
CPC classification number: C11B3/006 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N9/93 , C12N15/52 , C12N15/8202 , C12N15/8205 , C12N15/8216 , C12N15/8218 , C12N15/8234 , C12N15/8247 , C12N15/8257 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12Y114/00 , C12Y114/19 , C12Y114/19003 , C12Y602/01003
Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ω3 destaurases, Δ5 elongases and Δ6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.
Abstract translation: 本发明涉及在重组细胞如酵母或植物细胞中合成长链多不饱和脂肪酸，特别是二十碳五烯酸，二十二碳五烯酸和二十二碳六烯酸的方法。 还提供了产生长链多不饱和脂肪酸的重组细胞或植物。 此外，本发明涉及一组具有去饱和酶或延伸酶活性的新酶，其可用于合成长链多不饱和脂肪酸的方法中。 特别地，本发明提供具有新活性的ω3去饱和酶，Δ5延伸酶和Δ6去饱和酶。 还提供了用多种基因瞬时和/或稳定转化细胞，特别是植物细胞的方法和DNA构建体。
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公开(公告)号：EP3121193A2
公开(公告)日：2017-01-25
申请号：EP16186318.8
申请日：2011-07-18
Inventor： WINZER, Thilo , GRAHAM, Ian Alexander , WALKER, Tracey Carol
IPC: C07K14/415 , C12N15/82
CPC classification number: C12N15/8251 , A01H3/04 , C07K14/415 , C12N7/00 , C12N9/0071 , C12N9/0077 , C12N15/67 , C12N15/8218 , C12N15/8243 , C12P17/188 , C12Q1/6876 , C12Q2600/156 , C12Q2600/158 , C12Y114/00
Abstract: This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode cytochrome P450 polypeptides from a Papaver somniferum cultivar; uses in the production of noscapine and identification of poppy cultivars that include genes that comprise said nucleic acid molecules.
Abstract translation: 本公开涉及编码来自罂粟幼苗品种的细胞色素P450多肽的核酸分子的分离和测序; 用于生产诺斯卡品和鉴定包括包含所述核酸分子的基因的罂粟品种。
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3.发明公开TYPE II FATTY ACID SYSTHESIS ENZYMES IN REVERSE B-OXIDATION 审中-公开TYP-II-FETsÄYSYNTHESEENZYMEIN DER REVERSE-B-OXIDATION
公开(公告)号：EP3099763A1
公开(公告)日：2016-12-07
申请号：EP15740946.7
申请日：2015-01-26
Applicant: William Marsh Rice University
Inventor： GONZALEZ, Ramon , CLOMBURG, James, M. , VICK, Jacob, E.
IPC: C10L1/06
CPC classification number: C12P7/64 , C10L1/02 , C10L1/04 , C12N9/00 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0071 , C12N9/1029 , C12N9/1096 , C12N9/88 , C12N15/52 , C12Y101/011 , C12Y101/03013 , C12Y102/01 , C12Y102/01003 , C12Y103/01009 , C12Y114/00 , C12Y203/01009 , C12Y206/00 , C12Y401/99005 , C12Y402/01059 , Y02E50/343
Abstract: This disclosure describes enzymes from the type II (a discrete set of enzymes) fatty acid synthesis ("FAS") pathway that can be used in combination with thiolases to operate a functional reversal of the β-oxidation cycle. A combination of thiolases with one or more of 3-oxoacyl-[acyl-carrier-protein] reductase (FabG, others), 3-hydroxyacyl-[acp] dehydratase (FabA, FabZ, others), and enoyl-[acyl-carrier-protein] reductase (FabI, FabK, FabL, FabV, others) yields a functional reversal of the β-oxidation cycle. If only one or two enzymes are used, the remaining enzymes will be traditional beta oxidation enzymes. Once this cycle is coupled with the appropriate priming and termination pathways, the production of carboxylic acids, alcohols, hydrocarbons, amines and their α-, β-, and ω-functionalized derivatives from renewable carbon sources can be achieved.
Abstract translation: 本公开描述了可以与硫醇酶组合使用以操作β-氧化循环的功能性逆转的来自II型（一组离散酶）脂肪酸合成（“FAS”）途径的酶。 硫氧酶与一个或多个3-氧基酰基 - [酰基 - 载体 - 蛋白]还原酶（FabG，其他），3-羟基酰基 - [acp]脱水酶（FabA，FabZ等）和烯酰基 - - 蛋白]还原酶（FabI，FabK，FabL，FabV等）产生β-氧化循环的功能逆转。 如果只使用一种或两种酶，剩余的酶将是传统的β氧化酶。 一旦这个循环与适当的引发和终止途径相结合，就可以实现羧酸，醇，烃，胺及其可再生碳源的α-，β-和ω官能化衍生物的生产。
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公开(公告)号：EP3121193A3
公开(公告)日：2017-04-12
申请号：EP16186318.8
申请日：2011-07-18
Inventor： WINZER, Thilo , GRAHAM, Ian Alexander , WALKER, Tracey Carol
IPC: C07K14/415 , C12N15/82
CPC classification number: C12N15/8251 , A01H3/04 , C07K14/415 , C12N7/00 , C12N9/0071 , C12N9/0077 , C12N15/67 , C12N15/8218 , C12N15/8243 , C12P17/188 , C12Q1/6876 , C12Q2600/156 , C12Q2600/158 , C12Y114/00
Abstract: This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode cytochrome P450 polypeptides from a Papaver somniferum cultivar; uses in the production of noscapine and identification of poppy cultivars that include genes that comprise said nucleic acid molecules.
Abstract translation: 本公开涉及从罂粟种植体栽培品种编码细胞色素P450多肽的核酸分子的分离和测序; 用于生产诺斯卡品和鉴定包含所述核酸分子的基因的罂粟品种。
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5.发明公开CYTOCHROME P450 AND CYTOCHROME P450 REDUCTASE POLYPEPTIDES, ENCODING NUCLEIC ACID MOLECULES AND USES THEREOF 审中-公开细胞色素P450和细胞色素P450 REDUKTASEPOLYPEPTIDE，核酸分子编码及其用途
公开(公告)号：EP2914729A4
公开(公告)日：2016-11-02
申请号：EP13850891
申请日：2013-10-31
Applicant: UNIV BRITISH COLUMBIA , UNIV WESTERN AUSTRALIA
Inventor： BOHLMANN CARL JOERG , DIAZ CHAVEZ MARIA LUISA , MONIODIS JESSIE
IPC: C12N15/53 , A01H5/00 , C11B9/00 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N9/96 , C12P7/02
CPC classification number: C12N9/0042 , C11B9/00 , C12N9/0071 , C12P5/007 , C12P7/02 , C12P15/00 , C12Y106/02004 , C12Y114/00
Abstract: Provided are cytochrome P450 polypeptides, including cytochrome P450 santalene oxidase polypeptides, cytochrome P450 bergamotene oxidase polypeptides and cytochrome P450 reductase polypeptides. Also provided are nucleic acid molecules encoding the cytochrome P450 polypeptides. Cells containing the nucleic acids and/or the polypeptides are provided as are methods for producing terpenes, such as santalols and bergamotols, by culturing the cells.
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Patent Agency Ranking6.发明公开PROTEIN HAVING GLYCOALKALOID BIOSYNTHASE ACTIVITY, AND GENE ENCODING SAME 有权和作业和GLYCOALKALOIDBIOSYNTHASE基因编码的蛋白
公开(公告)号：EP2471927A4
公开(公告)日：2013-02-20
申请号：EP10812057
申请日：2010-08-30
Applicant: KIRIN HOLDINGS KK
Inventor： UMEMOTO NAOYUKI , SASAKI KATSUNORI
IPC: C12N9/00 , C07K14/415 , C12N15/82
CPC classification number: C12N15/8243 , C07K14/415 , C12N9/00 , C12N9/0071 , C12N15/8218 , C12Q1/6895 , C12Q2600/156 , C12Y114/00 , C12Y114/14001
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7.发明公开ENZYMES AND METHODS FOR PRODUCING OMEGA-3 FATTY ACIDS 有权芬芳VERFAHREN ZUR HERSTELLUNG VON OMEGA-3-FETTSÄUREN
公开(公告)号：EP2358882A4
公开(公告)日：2012-05-02
申请号：EP09827035
申请日：2009-11-17
Applicant: COMMW SCIENT IND RES ORG , GRAINS RES & DEV CORP
Inventor： PETRIE JAMES ROBERTSON , MACKENZIE ANNE MAREE , LIU QING , SHRESTHA PUSHKAR , NICHOLS PETER DAVID , BLACKBURN SUSAN IRENE ELLIS , MANSOUR MAGED PETER , ROBERT STANLEY SURESH , FRAMPTON DION MATTHEW FREDERICK , ZHOU XUE-RONG , SINGH SURINDER PAL , WOOD CRAIG CHRISTOPHER
CPC classification number: C11B3/006 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N9/93 , C12N15/52 , C12N15/8202 , C12N15/8205 , C12N15/8216 , C12N15/8218 , C12N15/8234 , C12N15/8247 , C12N15/8257 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12Y114/00 , C12Y114/19 , C12Y114/19003 , C12Y602/01003
Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides É3 destaurases, ”5 elongases and ”6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.
Abstract translation: 本发明一般涉及重组脂肪酸合成领域，特别是在转基因植物中。 本申请描述了脂肪酸合成中涉及的基因，并提供了用于操作植物油脂肪酸组成的方法和载体。 特别地，本发明提供了用于实现将参与脂肪酸合成的多个异源基因整合到植物基因组中的结构，使得所得植物产生多不饱和脂肪酸水平的改变。 还描述了通过共同表达植物储存器官内的沉默抑制剂来增强脂肪酸生物合成酶的表达的方法。
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公开(公告)号：EP2281046B1
公开(公告)日：2012-01-25
申请号：EP09750966.5
申请日：2009-05-19
Applicant: Promega Corporation
Inventor： ENCELL, Lance, P. , ZIMMERMAN, Kris , OTTO, Paul , VIDUGIRIS, Gediminas , STECHA, Pete , BINKOWSKI, Brock , WOOD, Monika, G. , WOOD, Keith, V.
IPC: C12N15/62 , C12N9/02 , C12N5/10 , C12Q1/26 , G01N33/573
CPC classification number: C12Q1/66 , C07K2319/20 , C07K2319/61 , C12N9/0004 , C12N9/0069 , C12Q1/008 , C12Q1/6876 , C12Y113/12 , C12Y114/00 , G01N33/5735 , G01N33/74 , G01N2333/726 , G01N2333/90241
Abstract: A modified luciferase protein which is a sensor for molecules including cAMP is provided. The modified luciferase protein includes one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with cAMP.
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公开(公告)号：EP2596012B1
公开(公告)日：2017-12-06
申请号：EP11748702.5
申请日：2011-07-18
Inventor： WINZER, Thilo , WALKER, Tracy Carol , GRAHAM, Ian Alexander
IPC: C07K14/415
CPC classification number: C12N15/8251 , A01H3/04 , C07K14/415 , C12N7/00 , C12N9/0071 , C12N9/0077 , C12N15/67 , C12N15/8218 , C12N15/8243 , C12P17/188 , C12Q1/6876 , C12Q2600/156 , C12Q2600/158 , C12Y114/00
Abstract: This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode cytochrome P450 polypeptides from a Papaver somniferum cultivar; uses in the production of noscapine and identification of poppy cultivars that include genes that comprise said nucleic acid molecules.
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10.发明公开
公开(公告)号：EP3099763A4
公开(公告)日：2017-08-02
申请号：EP15740946
申请日：2015-01-26
Applicant: UNIV RICE WILLIAM M
Inventor： GONZALEZ RAMON , CLOMBURG JAMES M , VICK JACOB E
IPC: C10L1/06
CPC classification number: C12P7/64 , C10L1/02 , C10L1/04 , C12N9/00 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0071 , C12N9/1029 , C12N9/1096 , C12N9/88 , C12N15/52 , C12Y101/011 , C12Y101/03013 , C12Y102/01 , C12Y102/01003 , C12Y103/01009 , C12Y114/00 , C12Y203/01009 , C12Y206/00 , C12Y401/99005 , C12Y402/01059 , Y02E50/343
Abstract: This disclosure describes enzymes from the type II (a discrete set of enzymes) fatty acid synthesis (“FAS”) pathway that can be used in combination with thiolases to operate a functional reversal of the β-oxidation cycle. A combination of thiolases with one or more of 3-oxoacyl-[acyl-carrier-protein] reductase (FabG, others), 3-hydroxyacyl-[acp] dehydratase (FabA, FabZ, others), and enoyl-[acyl-carrier-protein] reductase (FabI, FabK, FabL, FabV, others) yields a functional reversal of the β-oxidation cycle. If only one or two enzymes are used, the remaining enzymes will be traditional beta oxidation enzymes. Once this cycle is coupled with the appropriate priming and termination pathways, the production of carboxylic acids, alcohols, hydrocarbons, amines and their α-, β-, and ω-functionalized derivatives from renewable carbon sources can be achieved.
Abstract translation: 本公开描述了可以与硫解酶组合用于操作β-氧化循环的功能性反转的II型（一组离散的酶）脂肪酸合成（“FAS”）途径的酶。 硫解酶与一种或多种3-氧酰基 - [酰基 - 载体 - 蛋白]还原酶（FabG等），3-羟基酰基 - [acp]脱水酶（FabA，FabZ等）和烯酰基 - [酰基载体 蛋白]还原酶（FabI，FabK，FabL，FabV等）产生β-氧化循环的功能性反转。 如果只使用一种或两种酶，剩余的酶将是传统的β氧化酶。 一旦该循环与适当的引发和终止途径相结合，可以实现从可再生碳源生产羧酸，醇，烃，胺和它们的α-，β-和ω-官能化衍生物。
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