公开(公告)号：EP1188760A4

公开(公告)日：2002-08-07

申请号：EP00929862

申请日：2000-05-25

申请人： DAIICHI SEIYAKU CO ,  NAGANO TESTUO

发明人： NAGANO TETSUO ,  KIKUCHI KAZUYA ,  INOUE TAKANARI ,  IINO MASAMITSU ,  HIROSE KENZO

IPC分类号： A61P43/00 ,  C07F9/117 ,  C07F9/655 ,  C07F9/6561 ,  C07F9/144 ,  A61K31/661 ,  A61K41/00 ,  C07D493/10

CPC分类号： C07F9/6561 ,  C07F9/117 ,  C07F9/65522

摘要： Compounds of general formula (I) or salts thereof, useful as ligands to inositol 1,4,5-trisphosphate (IP3) receptor, wherein R is a group represented by general formula (A), (B) or (C) (wherein R and R are each independently C1-6 alkyl; R is amino, mono(C1-6 alkyl)amino, di(C1-6 alkyl)amino, or C1-6 alkoxy; and X is an anion); and n is an integer of 2 to 5.

公开(公告)号：EP1000941A4

公开(公告)日：2002-07-31

申请号：EP98929754

申请日：1998-06-30

申请人： NAGANO TESTUO

发明人： NAGANO TETSUO ,  KOJIMA HIROTATSU ADACHI

IPC分类号： A61K31/352 ,  C07D249/04 ,  C07D311/82 ,  C07D311/88 ,  C07D311/96 ,  C07D493/10 ,  G01N31/22 ,  G01N33/00 ,  C07D311/84 ,  C07D311/90 ,  G01N21/78 ,  G01N33/52

CPC分类号： G01N31/223 ,  C07D311/82 ,  C09B11/24 ,  Y10T436/177692

摘要： Compounds represented by general formula (1), and reagents containing the same for determining nitrogen monoxide (wherein R?1 and R2¿ are amino groups bonded to the phenyl ring at positions adjacent to each other; R?3, R4, R5 and R6¿ are each independently C¿1?-C6 alkyl; R?7, R8, R9 and R10¿ are each independently hydrogen, C¿1?-C6 alkyl, allyl or halogeno; R?11¿ is hydrogen or C¿1?-C18 alkyl; and X?-¿ is an anion). The compounds react with nitrogen monoxide efficiently to produce fluorescent compounds which emit intensive fluorescence when irradiated with a long-wavelength excitation light. The fluorescent compounds are detectable in a fluorescence region wherein little influence of autofluorescence of cells is observed, and characterized in that the intensity of fluorescence is not lowered even under acid conditions.

摘要翻译： 通式（1）表示的化合物和含有其的用于测定一氧化氮的试剂（其中R 1和R 2是彼此相邻位置与苯环键合的氨基; R 3，R 4，R 5和R 6 各自独立地为C 1 -C 6烷基; R 7，R 8，R 9和R 10各自独立地为氢，C 1 -C 6烷基，烯丙基或卤代; R 11为氢或C 1〜 -C 18烷基;并且X 5是一个阴离子）。 这些化合物与一氧化氮高效地反应生成荧光化合物，当用长波长激发光照射时，荧光化合物发出强烈的荧光。 荧光化合物在荧光区域中可检测到，其中观察到细胞自发荧光的影响很小，并且特征在于即使在酸性条件下荧光强度也不降低。

公开(公告)号：EP1069121A4

公开(公告)日：2002-08-28

申请号：EP99912055

申请日：1999-03-31

申请人： NAGANO TESTUO

发明人： NAGANO TETSUO ,  UMEZAWA NAOKI

IPC分类号： C07D311/82 ,  C07D493/08 ,  C07D493/10 ,  C07D493/22 ,  G01N31/22 ,  G01N21/78 ,  G01N33/48

CPC分类号： C07D493/08 ,  C07D311/82 ,  C07D493/10 ,  G01N31/225 ,  Y10S436/80

摘要： Compounds represented by general formula (I) or salts thereof, wherein R?1, R2, R3, R4, R5, and R6¿ each independently represents hydrogen, halogeno, C¿1-6? alkyl, or C1-6 alkoxyl; R?7 and R8¿ each independently represents C¿1-6? alkyl or optionally substituted aryl; R?9 and R10¿ each independently represents hydrogen, C¿1-6? alkyl, or C1-6 alkoxyl; and R?11¿ represents hydrogen or C¿1-12? alkanoyl. The compounds are substantially nonfluorescent and react with singlet oxygen under physiological conditions to give a fluorescent substance. They are hence useful as a reagent for singlet oxygen determination.