公开(公告)号：EP2935613B1

公开(公告)日：2020-05-27

申请号：EP13864616.1

申请日：2013-12-19

Applicant: DNAE Group Holdings Limited

Inventor： CLARIZIA, Lisa-jo, Ann ,  ADAMS, Eddie, W. ,  DRYGA, Sergey, A. ,  NORVELL, Meghan, E. ,  DYKES, Colin ,  BARR, Alexandra ,  SITDIKOV, Ravil, A. ,  TORRANCE, Magdalena, A. ,  ALEY, David, K. ,  SMITH, Erik, J. ,  ESCH, Victor, C. ,  MACEMON, James, H. ,  VANDERVEST, Jaclyn

IPC: G01N33/543 ,  C12Q1/24 ,  B01L3/00 ,  G01N33/569

公开(公告)号：EP2561354B1

公开(公告)日：2018-06-06

申请号：EP11772698.4

申请日：2011-04-21

Applicant: DNAE Group Holdings Limited

Inventor： ESCH, Victor, C. ,  DRYGA, Sergey, A. ,  CLARIZIA, Lisa-jo, Ann

IPC: G01N33/48

CPC classification number: G01N33/54326 ,  A61B5/055 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2400/043 ,  C07K1/22 ,  C07K16/1267 ,  C12N13/00 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q2565/633 ,  G01N24/08 ,  G01N33/54333 ,  G01N33/56911 ,  G01N2333/195 ,  G01N2446/20 ,  G01R33/465 ,  Y10T428/2982 ,  C12Q2565/629 ,  C12Q2563/143

Abstract: The invention generally relates to using magnetic particles and alternating magnet fields to separate a target analyte from a sample. In certain embodiments, methods of the invention involve contacting a sample with magnetic particles including first moieties specific for a target analyte, thereby forming target/particle complexes in the sample, flowing the sample through a channel including second moieties attached to at least one surface of the channel, applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface to bind the second moieties and unbound particles remaining free in the sample, binding the target/particle complexes to the second moieties, and washing away unbound particles and unbound analytes of the sample.