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1.发明公开PLURIPOTENT STEM CELL-DERIVED BROWN ADIPOCYTES, PLURIPOTENT STEM CELL-DERIVED CELL AGGREGATE, METHOD FOR PRODUCING SAME, AND CELL THERAPY AND MEDICAL THERAPY THEREFOR 审中-公开关多能干WON褐色脂肪细胞OFF多能干获得的细胞单元，加工用于生产和细胞治疗和医学治疗中的
公开(公告)号：EP2703481A4
公开(公告)日：2014-11-12
申请号：EP12776134
申请日：2012-04-26
发明人： SAEKI KUMIKO , YUO AKIRA , NISHIO MIWAKO , KAWASAKI MASAKO , SAEKI KOICHI , HASEGAWA MAMORU
CPC分类号： A61K35/35 , A61K35/28 , C12N5/0653 , C12N2501/105 , C12N2501/155 , C12N2501/165 , C12N2501/2306 , C12N2501/26 , C12N2506/45
摘要： Provided are a method of producing brown adipocytes from pluripotent stem cells, a method of producing cell aggregates as an intermediate product thereof, pluripotent stem cell-derived cell aggregates and pluripotent stem cell-derived brown adipocytes produced by these methods, and cell therapy using the pluripotent stem cell-derived brown adipocytes. In the method of producing brown adipocytes from pluripotent stem cells, cell aggregates are produced from pluripotent stem cells by a method including the step (A), and brown adipocytes are prepared from the cell aggregates by a method including the step (B). The step (A) is a step of producing cell aggregates by non-adhesive culture of pluripotent stem cells in serum-free environment in the presence of a hematopoietic cytokine, and the step (B) is a step of producing brown adipocytes by adhesion culture of the cell aggregates in the presence of a hematopoietic cytokine.
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公开(公告)号：EP2363472A4
公开(公告)日：2012-08-01
申请号：EP09823696
申请日：2009-10-30
申请人： DNAVEC CORP
发明人： INOUE MAKOTO , SAEKI KOICHI , YOU JUN , TABATA TOSHIAKI , IWASAKI HITOSHI , SHU TSUGUMINE , HASEGAWA MAMORU
IPC分类号： C12N15/09 , A61K35/76 , A61K48/00 , A61P43/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/62 , C12N15/67 , C12P21/02
CPC分类号： C12N15/67 , A61K2039/53 , C07K2319/55 , C12N15/86 , C12N2760/18843
摘要： The present invention provides methods for enhancing protein expression from an RNA viral vector and RNA viral vectors with enhanced protein expression capacity. The present inventors successfully increased the level of protein expression significantly by expressing the proteins fused with an AB5B protein from RNA viral vectors. Effective gene therapy, gene vaccination, monoclonal antibody preparation, or such can be achieved by using a gene transfer RNA viral vector of the present invention.
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3.发明公开HIGHLY FUNCTIONAL LIVER CELLS DERIVED FROM PLURIPOTENT STEM CELLS, METHOD FOR PRODUCING SAME, AND METHOD FOR TESTING METABOLISM/TOXICITY OF DRUG 审中-公开关多能干衍生的高功能肝细胞，用于用于测试的代谢毒性毒品生产和方法
公开(公告)号：EP2671944A4
公开(公告)日：2014-12-03
申请号：EP12742430
申请日：2012-01-30
发明人： YUO AKIRA , SAEKI KUMIKO , NAKAMURA NAOKO , MATSUYAMA SATOKO , NISHIO MIWAKO , SAEKI KOICHI , HASEGAWA MAMORU
CPC分类号： C12N5/067 , C12N2500/90 , C12N2501/115 , C12N2501/155 , C12N2501/16 , C12N2501/237 , C12N2501/39 , C12N2501/41 , C12N2501/415 , C12N2506/02 , C12N2506/45 , G01N33/5014 , G01N33/5067
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公开(公告)号：EP2612911A4
公开(公告)日：2014-03-12
申请号：EP11821786
申请日：2011-08-30
申请人： DNAVEC CORP
发明人： BAN HIROSHI , UEDA YASUJI , FUSAKI NOEMI , SAEKI KOICHI , HASEGAWA MAMORU
CPC分类号： C12N15/86 , C12N5/0696 , C12N15/63 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2506/13 , C12N2510/00 , C12N2760/18843
摘要： The present invention provides Sendai virus vectors in which genes that encode reprograming factors for inducing pluripotent stem cells are incorporated in a specific order, compositions comprising these vectors for gene delivery to be used in the induction of pluripotent stem cells, and uses thereof. Incorporation of the KLF gene, OCT gene, and SOX gene in a specific order into a single Sendai virus vector successfully and significantly increased the efficiency of pluripotent stem cell induction. Loading multiple reprogramming factors into a single vector can further increase the induction efficiency of pluripotent stem cells while reducing the number of necessary vectors.
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