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    Structuring hydrogels
    3.
    发明公开
    Structuring hydrogels 有权
    Strukturierung von Hydrogelen

    公开(公告)号：EP1864777A1

    公开(公告)日：2007-12-12

    申请号：EP06011868.4

    申请日：2006-06-08

    申请人： DWI an der RWTH Aachen e.V.

    发明人： Lensen, Marga ,  Möller, Martin

    IPC分类号： B29C33/38

    CPC分类号： B29C33/3878 ,  B29C33/405 ,  B29C33/424 ,  B82Y10/00 ,  B82Y40/00 ,  G03F7/0002

    摘要： The present invention relates to a method for the preparation of a surface structured bulk hydrogel bearing a topographic and chemical pattern, comprising the steps of:
    (i) providing a primary master having a predetermined topographic pattern,
    (ii) replicating said primary master by an elastomeric secondary master to obtain a secondary master with said predetermined topographic pattern in its inverse form,
    (iii) replicating said secondary master by an imprintable curable material of hydrogel precursor molecules which are capable of forming a hydrogel once crosslinked by curing, and crosslinking said material to thereby yield a replica made of cross-linked bulk hydrogel bearing the topographic pattern identical to that of the primary master, and
    (iv) functionalizing, particularly biofunctionalizing, the bulk hydrogel replica.
    In particular, the method according to the present invention can further comprise the step of:
    - functionalizing the protrusions of the topographically structured bulk hydrogel ("inking"),
    - functionalizing the depressions of the topographically structured bulk hydrogel ("print-molding"), or
    - functionalizing the topographically structured bulk hydrogel with a chemical pattern via a metal template on either the primary or secondary master ("transfer-molding"),

    to thereby specifically and locally functionalize the bulk hydrogel surface with a chemical pattern.

    摘要翻译： 本发明涉及一种用于制备具有地形和化学图案的表面结构的主体水凝胶的方法，其包括以下步骤：（i）提供具有预定地形图案的主母板，（ii）通过 以获得具有相反形式的所述预定地形图案的次级母版，（iii）通过水凝胶前体分子的可压印可固化材料复制所述次级母版，所述可水解凝胶前体分子能够通过固化形成水凝胶一次交联，并交联所述材料 从而产生由交联体积水凝胶制成的复制品，其具有与初级母料相同的地形图案，和（iv）主体水凝胶复制品的功能化，特别是生物功能化。 特别地，根据本发明的方法还可以包括以下步骤： - 将地形结构的主体水凝胶（“着色”）的突起功能化， - 将表面结构化的主体水凝胶（“印刷成型”）的凹陷功能化， 或 - 通过主母模或次母模上的金属模板（“转移模塑”）以化学图案使表面结构化的主体水凝胶官能化，从而以化学图案特异性地和局部地官能化本体水凝胶表面。

    Novel biosensor system based on recognition induced birefringence (RIB)
    4.
    发明公开
    Novel biosensor system based on recognition induced birefringence (RIB) 有权
    Neuartiges Biosensorsystem basierend auf der Erkennung von induzierter Doppelbrechung（RIB）

    公开(公告)号：EP2204650A1

    公开(公告)日：2010-07-07

    申请号：EP08022585.7

    申请日：2008-12-31

    申请人： DWI an der RWTH Aachen e.V.

    发明人： Groll, Jürgen, Dr. ,  Möller, Martin ,  Eberhardt, Matthias, Dr.

    IPC分类号： G01N33/543 ,  B01J19/00

    CPC分类号： G01N33/54393 ,  B01J2219/00509 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00623 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00702 ,  G01N33/54373

    摘要： The present invention relates to a label-free biosensor system, a method for manufacturing said label-free biosensor system, its use for detecting biochemical reactions and/or bindings, enzymatic reactions, nucleic acid hybridizations, protein-protein interactions and protein-ligand interactions, as well as an assay method for detecting and/or quantifying an analyte of interest in a biological sample which comprises detecting the Recognition Induced Birefringence (RIB) generated in the presence as opposed to the absence of said analyte by bringing said sample into contact with said label-free biosensor system.

    摘要翻译： 本发明涉及无标记生物传感器系统，制造所述无标记生物传感器系统的方法，其用于检测生化反应和/或结合的用途，酶反应，核酸杂交，蛋白质 - 蛋白质相互作用和蛋白质 - 配体相互作用 以及用于检测和/或定量生物样品中感兴趣分析物的测定方法，其包括检测在存在时产生的识别诱导双折射（RIB），而不是通过使所述样品与所述分析物接触而不存在所述分析物 所述无标签的生物传感器系统。