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公开(公告)号：EP2408910B1
公开(公告)日：2018-04-25
申请号：EP10709080.5
申请日：2010-03-16
申请人： Danisco US Inc.
发明人： PUNT, Peter, J. , BURLINGAME, Richard, Paul , PYNNONEN, Christine, M. , OLSON, Phillip,T. , WERY, Jan , VISSER, Johannes, Heinrich , EMALFARB, Mark, A. , VISSER, Jacob , VERDOES, Jan, Cornelis
CPC分类号： C12N15/80 , C12N1/14 , C12N9/1205 , C12N9/2402 , C12N9/2428 , C12N9/2437 , C12N9/2442 , C12N9/248 , C12N15/01 , C12P21/02 , C12Q1/40 , C12R1/645 , C12Y302/01003 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01014 , C12Y302/01015 , C12Y302/01091 , C12Y304/21062 , C12Y304/23025 , G01N2333/93
摘要： The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous &bgr;-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided.
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公开(公告)号：EP2183363B1
公开(公告)日：2016-06-08
申请号：EP08797046.3
申请日：2008-08-01
申请人： Danisco US Inc.
发明人： GUSAKOV, Alexander, Vasilievich , PUNT, Peter, J. , VERDOES, Jan, Cornelis , SINITSYN, Arkady, Panteleimonovich , VLASENKO, Elena , HINZ, Sandra Wihelmina, Agnes , GOSINK, Mark , JIANG, Zhijie
CPC分类号： C12Y302/01021 , C11D3/38645 , C12N9/2437 , C12N9/2442 , C12N9/2445 , C12N9/2477 , C12N9/248 , C12P7/10 , C12P19/14 , C12Y302/01003 , C12Y302/01004 , C12Y302/01014 , C12Y302/01032 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： This invention relates to enzymes and methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, deinking and biobleaching of paper and pulp, and treatment of waste streams.
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