GLYCOSYLATION AS A STABILIZER FOR PHYTASE
    4.
    发明公开
    GLYCOSYLATION AS A STABILIZER FOR PHYTASE 审中-公开
    GLYCOSYLIERUNG ALS STABILISATORFÜRPHYTASE

    公开(公告)号:EP2812429A1

    公开(公告)日:2014-12-17

    申请号:EP13704695.9

    申请日:2013-02-01

    申请人: Danisco US Inc.

    IPC分类号: C12N9/14

    摘要: The present teachings provide modified enzymes, preferably phytases, which have increased stability, hypothesized to arise from increased glycosylation. The enzymes can be modified to introduce or increase the number of glycosylation sites in the amino acid sequence, or glycosylation can be increased by the use of specific host production methods, or both. The enzymes of the present teachings have an increased stability after treatment at elevated temperature, which can be measured by inactivity reversibility or percent recovery following a treatment such as heating. The enzymes of the present teachings find application for example in food, feed, and feed pelleting.

    摘要翻译: 本教导提供修饰的酶,优选植酸酶,其具有增加的稳定性,假定由增加的糖基化引起。 可以修饰酶以引入或增加氨基酸序列中的糖基化位点的数目,或者可以通过使用特定的宿主生产方法或两者来增加糖基化。 本教导的酶在升高的温度下处理后具有增加的稳定性,其可以通过不能变化的可逆性或在加热等处理后的百分比恢复来测量。 本发明的酶可用于例如食品,饲料和饲料造粒。