公开(公告)号：EP2844754B1

公开(公告)日：2018-10-24

申请号：EP13784528.5

申请日：2013-05-02

申请人： Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

发明人： SHUKLA, Vipula ,  GUPTA, Manju ,  URNOV, Fyodor ,  GUSCHIN, Dmitry ,  JAN DE BOTH, Michiel ,  BUNDOCK, Paul ,  SASTRY-DENT, Lakshmi

IPC分类号： C12N15/05 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N9/0006 ,  A01C14/00 ,  C12N15/8213 ,  C12N15/8241 ,  C12N15/8243 ,  C12N15/8261 ,  C12N15/8269 ,  C12Y101/01037 ,  C12Y301/00 ,  Y02A40/146

摘要： Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.

公开(公告)号：EP2893006B1

公开(公告)日：2018-08-22

申请号：EP13834682.0

申请日：2013-09-05

申请人： Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

发明人： COGAN, Noel ,  FORSTER, John ,  HAYDEN, Matthew ,  SAWBRIDGE, Tim ,  SPANGENBERG, German ,  WEBB, Steven R. ,  GUPTA, Manju ,  AINLEY, William Michael ,  HENRY, Matthew J. ,  MILLER, Jeffrey C. ,  GUSCHIN, Dmitry Y.

IPC分类号： C12N5/10

CPC分类号： C12N15/79 ,  C12N9/22 ,  C12N15/63 ,  C12N15/8241 ,  C12N15/8247

摘要： A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：EP2893023B1

公开(公告)日：2018-08-15

申请号：EP13834887.5

申请日：2013-09-05

申请人： Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

发明人： COGAN, Noel ,  FORSTER, John ,  HAYDEN, Matthew ,  SAWBRIDGE, Tim ,  SPANGENBERG, German ,  WEBB, Steven, R. ,  GUPTA, Manju ,  AINLEY, William, Michael ,  HENRY, Matthew, J. ,  MILLER, Jeffrey, C. ,  GUSCHIN, Dmitry, Y.

IPC分类号： C12N15/10 ,  C12N15/82 ,  C12N15/90 ,  C12N5/04 ,  C12N9/22 ,  A01H1/06 ,  A01H5/00

CPC分类号： C12N15/8216 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/66 ,  C12N15/822 ,  C12N15/8247 ,  C12N15/902 ,  C12Y301/00

摘要： A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：EP3072973B1

公开(公告)日：2018-11-28

申请号：EP16163658.4

申请日：2008-09-25

申请人： Dow AgroSciences, LLC ,  Sangamo Therapeutics, Inc.

发明人： GUPTA, Manju ,  PALTA, Asha M. ,  NOVAK, Stephen ,  URNOV, Fyodor ,  GOPALAN, Sunita

IPC分类号： C12N15/82 ,  C12N9/22 ,  C12N15/90 ,  A01H5/00

CPC分类号： C12N15/8213 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/1092 ,  C12N15/8243 ,  C12N15/8274

摘要： The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

公开(公告)号：EP3052633B1

公开(公告)日：2019-06-12

申请号：EP14850603.3

申请日：2014-10-02

申请人： Dow AgroSciences LLC

发明人： GUPTA, Manju ,  KUMAR, Sandeep ,  ELANGO, Navin ,  MUTHURAMAN, Karthik, Narayna ,  BERINGER, Jeffrey ,  WU, Huixia ,  SARDESAI, Nagesh

IPC分类号： C12N15/82

公开(公告)号：EP3058073B1

公开(公告)日：2018-12-12

申请号：EP14853513.1

申请日：2014-10-15

申请人： Dow AgroSciences LLC

发明人： GUPTA, Manju ,  ELANGO, Navin ,  MUTHURAMAN, Karthik Narayna ,  BERINGER, Jeffrey ,  BENNETT, Sara ,  WU, Huixia ,  GORMAN, Shavell ,  WORDEN, Andrew

IPC分类号： C12N15/82 ,  C12N5/00 ,  C12N5/02 ,  C12N5/10

CPC分类号： C12N15/8216 ,  C12N15/113 ,  C12N15/8222 ,  C12N15/8237 ,  C12N15/8241

摘要： Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5′-UTRs, and/or 3′-UTRs, isolated from Zea mays.

公开(公告)号：EP2877581B1

公开(公告)日：2017-11-29

申请号：EP13744922.9

申请日：2013-07-23

申请人： Dow AgroSciences LLC

发明人： KUMAR, Sandeep ,  EVANS, Steven, L. ,  GUPTA, Manju

IPC分类号： C12N15/63

CPC分类号： C12N15/8209 ,  C12N15/68 ,  C12N15/82

公开(公告)号：EP2885412A2

公开(公告)日：2015-06-24

申请号：EP13752757.8

申请日：2013-08-08

申请人： Dow AgroSciences LLC ,  Kumar, Sandeep ,  Gupta, Manju ,  Alabed, Diaa

发明人： KUMAR, Sandeep ,  GUPTA, Manju ,  ALABED, Diaa

IPC分类号： C12N15/82

CPC分类号： C12N15/8216 ,  C12N15/8218

摘要： Provided are methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 3′ untranslated regions of genes encoding ubiquitin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional or gene targeting procedures, resulting in enhanced expression of the selected coding region. In some embodiments, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

摘要翻译： 提供了用于增强转基因植物和植物组织中重组核酸序列表达的方法，载体和基因构建体。 根据本发明，获得核酸序列和/或衍生自编码遍在蛋白质的基因的3'非翻译区并且被工程化成位于载体的所选编码区的相应部分。 可以通过常规或基因靶向程序将载体构建体引入植物和/或植物组织中，导致选择的编码区的表达增强。 在一些实施方案中，所选择的编码区是表达一种或多种已知赋予转基因植物和/或植物组织杀虫活性水平的蛋白质的嵌合基因或基因片段。

公开(公告)号：EP2859104A2

公开(公告)日：2015-04-15

申请号：EP13800385.0

申请日：2013-06-05

申请人： Dow AgroSciences LLC

发明人： GUPTA, Manju ,  RUSSELL, Sean Michael ,  SHEN, Liu Yin ,  CHENNAREDDY, Sivarama Reddy ,  ROUT, Jyoti R. ,  NOVAK, Stephen

IPC分类号： C12N15/82 ,  C12N15/113 ,  C12N5/14 ,  C07H21/04

CPC分类号： C07K14/415 ,  C12N15/8216

摘要： Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues using a disclosed bidirectional promoter from
Brassica napus or
Brassica bidirectional constitutive promoter (BBCP). The constructs provided comprise at least one such bi-directional promoter linked to multiple gene expression cassettes, wherein each of the gene expression cassettes comprises at least one transgene. In some embodiments, the constructs and methods provided allow expression of genes between two and twenty.

公开(公告)号：EP2491127A1

公开(公告)日：2012-08-29

申请号：EP10825333.7

申请日：2010-10-22

申请人： Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

发明人： DEKELVER, Russell ,  GUPTA, Manju ,  MILLER, Jeffrey C. ,  NOVAK, Stephen ,  PETOLINO, Joseph F.

IPC分类号： C12N15/82 ,  C12N5/04 ,  A01H5/00

CPC分类号： C12N15/8218 ,  C07K14/415 ,  C12N9/22 ,  C12N15/8216 ,  C12N15/8247 ,  C12Y203/01041 ,  C12Y301/00

摘要： The present dislosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.