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公开(公告)号:EP2421963B1
公开(公告)日:2016-11-30
申请号:EP10723329.8
申请日:2010-04-23
发明人: MADRID, Susan M , LIN, Cherry , ZARGAHI, Masoud Rajabi , LORENTSEN, Rikke Høegh , ISAKSEN, Mai Faurschou , WARD, Michael
IPC分类号: A23L29/00 , A23K20/189 , C12N9/20 , C12N15/80 , C12R1/885
CPC分类号: C12N9/20 , A23K20/189 , A23L29/06 , A23L33/14 , A23V2002/00 , C12N15/80 , C12Y301/01003
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公开(公告)号:EP2421386B1
公开(公告)日:2016-05-25
申请号:EP10719104.1
申请日:2010-04-23
发明人: ISAKSEN, Mai Faurschou , LORENTSEN, Rikke Høegh , PLUMSTEAD, Peter , ROMERO MILLÁN, Luis Fernando , MADRID, Susan , LIN, Cherry , WARD, Michael , ZARGAHI, Masoud Rajabi
IPC分类号: A23K20/189 , A23L33/10 , A61K38/46 , C12N9/16 , A23K50/30 , A23K50/75 , A23K50/80 , C12N9/20
CPC分类号: A23L1/30 , A23K20/189 , A23K50/30 , A23K50/75 , A23L33/10 , A61K38/465 , C12N9/16 , C12N9/20
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公开(公告)号:EP2879511A1
公开(公告)日:2015-06-10
申请号:EP13744572.2
申请日:2013-08-02
发明人: ISAKSEN, Mai Faurschou , BERNARDEAU, Marion , MILLÁN, Luis Fernando Romero , KIARIE, Elijah Gituanjah , ARENT, Susan Lund , NURMINEN, Päivi Helena , FORSSTEN, Sofia , DAVIS, Mari Ellen , PETRI, Daniel , GALBRAITH, Elizabeth Ann
CPC分类号: A23K10/18 , A23K10/16 , A23K20/189 , A23K50/30 , A23K50/60 , A23K50/75 , A23Y2220/00 , A61K35/742 , A61K35/747 , A61K38/47 , C12Y302/01008 , C12Y302/01021
摘要: A feed additive composition comprising a direct fed microbial (DFM), in combination with a xylanase (e.g. endo-1,4-&bgr;-d-xylanase) and a &bgr;-glucanase (and optionally a further fibre degrading enzyme), wherein the DFM is selected from the group consisting of an enzyme producing strain; a C5 sugar-fermenting strain; a short-chain fatty acid-producing strain; a fibrolytic, endogenous microflora-promoting strain; or combinations thereof. The DFM may be selected from the group consisting of: Bacillus subtilis AGTP BS3BP5, Bacillus subtilis AGTP BS442, B. subtilis AGTP BS521, B. subtilis AGTP BS918, Bacillus subtilis AGTP BS1013, B. subtilis AGTP BS1069, B. subtilis AGTP 944, B. pumilus AGTP BS 1068 or B. pumilus KX11-1, Enterococcus faecium ID7, Propionibacterium acidipropionici P169, Lactobacillus rhamnosus CNCM-1-3698, Lactobacillus farciminis CNCM-1-3699, a strain having all the characteristics thereof, any derivative or variant thereof, and combinations thereof and the further fibre degrading enzyme may be selected from the group consisting of a cellobiohydrolase (E.C. 3.2.1.176 and E.C. 3.2.1.91), a &bgr;-glucosidase (E.C. 3.2.1.21), a &bgr;-xylosidase (E.C. 3.2.1.37), a feruloyl esterase (E.C. 3.1.1.73), an α-arabinofuranosidase (E.C. 3.2.1.55), a pectinase (e.g. an endopolygalacturonase (E.C. 3.2.1.15), an exopolygalacturonase (E.C. 3.2.1.67) or a pectate lyase (E.C. 4.2.2.2)), or combinations thereof.
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公开(公告)号:EP3486656A1
公开(公告)日:2019-05-22
申请号:EP18193539.6
申请日:2012-06-22
发明人: ISAKSEN, Mai Faurschou , KELLETT-SMITH, Anja Hemmingsen , MCLENNAN, Neil , GILBERT, Ceinwen , KYNEB, Majbritt Hauge
IPC分类号: G01N33/558 , G01N33/573 , C12Q1/42
摘要: The present invention discloses an assay device (1) for detecting active enzyme in a sample. Said assay device (1) comprises the following components:- (a) a placement region (10) onto which the sample can be placed; (b) a matrix (20) operably connected to said placement region (10) such that the sample when present (such as placed) on said placement region (10) can migrate along said matrix (20); (c) at least one distinct capture location (30) on said matrix (20), wherein each distinct capture location (30) is distanced away from the placement region (10), and wherein the sample can migrate across said distinct capture location (30); (d) capture means (40) being present at or defining each distinct capture location (30), wherein said capture means (40) are capable of binding to said enzyme such that at least a portion of said sample of said enzyme is retained at at least one distinct capture location (30); and (e) selective indication means (50), or at least a component thereof, to provide selective indication of the presence of active enzyme bound to said capture means (40).
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公开(公告)号:EP2729809B1
公开(公告)日:2018-10-17
申请号:EP12731634.7
申请日:2012-06-22
发明人: ISAKSEN, Mai Faurschou , KELLETT-SMITH, Anja Hemmingsen , MCLENNAN, Neil , GILBERT, Ceinwen , KYNEB, Majbritt Hauge
IPC分类号: G01N33/558 , G01N33/573 , C12Q1/42
CPC分类号: G01N33/573 , C12Q1/00 , C12Q1/34 , C12Q1/42 , G01N33/558 , G01N2333/914 , G01N2333/916 , G01N2333/924
摘要: The present invention discloses an assay device (1) for detecting active enzyme in a sample. Said assay device (1) comprises the following components:—(a) a placement region (10) onto which the sample can be placed; (b) a matrix (20) operably connected to said placement region (10) such that the sample when present (such as placed) on said placement region (10) can migrate along said matrix (20); (c) at least one distinct capture location (30) on said matrix (20), wherein each distinct capture location (30) is distanced away from the placement region (10), and wherein the sample can migrate across said distinct capture location (30); (d) capture means (40) being present at or defining each distinct capture location (30), wherein said capture means (40) are capable of binding to said enzyme such that at least a portion of said sample of said enzyme is retained at at least one distinct capture location (30); and (e) selective indication means (50), or at least a component thereof, to provide selective indication of the presence of active enzyme bound to said capture means (40).
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公开(公告)号:EP2591123B1
公开(公告)日:2016-10-05
申请号:EP11745831.5
申请日:2011-07-07
CPC分类号: C12N9/2417 , A23K20/174 , A23K20/189 , A23K20/20 , A23K40/10 , A23K40/25 , A23K40/30 , A23K50/30 , A23K50/75 , A23K50/80 , C07K2319/02 , C12Q1/40 , C12Y302/01001 , Y02A40/818 , Y02P60/877
摘要: The present invention relates to a method for identifying a pepsin resistant alpha amylase enzyme for use in a feed supplement comprising: i) providing an alpha amylase enzyme; ii) admixing said alpha amylase with com based feed and buffer solution comprising a pepsin concentration of 9000 U/ml at pH 3, 40° C., 500 rpm for al least 120 minutes and analysing alpha amylase activity on said alpha amylase compared to a control sample; wherein said control sample differs in that no pepsin is present during incubation; and iii) selecting an alpha amylase enzyme which substantially maintains alpha amylase activity under the assay conditions; feed supplements and feed stuffs comprising a pepsin resistant alpha amylase and the use of pepsin resistant alpha amylases in feed.
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公开(公告)号:EP2729809A1
公开(公告)日:2014-05-14
申请号:EP12731634.7
申请日:2012-06-22
发明人: ISAKSEN, Mai Faurschou , KELLETT-SMITH, Anja Hemmingsen , MCLENNAN, Neil , GILBERT, Ceinwen , KYNEB, Majbritt Hauge
IPC分类号: G01N33/558 , G01N33/573 , C12Q1/42
CPC分类号: G01N33/573 , C12Q1/00 , C12Q1/34 , C12Q1/42 , G01N33/558 , G01N2333/914 , G01N2333/916 , G01N2333/924
摘要: The present invention discloses an assay device (1) for detecting active enzyme in a sample. Said assay device (1) comprises the following components:—(a) a placement region (10) onto which the sample can be placed; (b) a matrix (20) operably connected to said placement region (10) such that the sample when present (such as placed) on said placement region (10) can migrate along said matrix (20); (c) at least one distinct capture location (30) on said matrix (20), wherein each distinct capture location (30) is distanced away from the placement region (10), and wherein the sample can migrate across said distinct capture location (30); (d) capture means (40) being present at or defining each distinct capture location (30), wherein said capture means (40) are capable of binding to said enzyme such that at least a portion of said sample of said enzyme is retained at at least one distinct capture location (30); and (e) selective indication means (50), or at least a component thereof, to provide selective indication of the presence of active enzyme bound to said capture means (40).
摘要翻译: 本发明公开了用于检测样品中的活性酶的测定装置(1)。 所述测定装置(1)包括以下部件:(a)放置样品的放置区域(10); (b)可操作地连接到所述放置区域(10)的矩阵(20),使得当存在(例如放置)在所述放置区域(10)上时,所述试样能够沿着所述矩阵(20)迁移; (c)在所述矩阵(20)上的至少一个不同的捕获位置(30),其中每个不同的捕获位置(30)远离所述放置区域(10),并且其中所述样品可以迁移穿过所述不同的捕获位置 30); (d)存在于或限定每个不同捕获位置(30)的捕获装置(40),其中所述捕获装置(40)能够结合所述酶,使得所述酶的所述样品的至少一部分保留在 至少一个不同的捕获位置(30); 和(e)选择性指示装置(50)或其至少一个组分,以提供与所述捕获装置(40)结合的活性酶的存在的选择性指示。
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公开(公告)号:EP2591123A2
公开(公告)日:2013-05-15
申请号:EP11745831.5
申请日:2011-07-07
CPC分类号: C12N9/2417 , A23K20/174 , A23K20/189 , A23K20/20 , A23K40/10 , A23K40/25 , A23K40/30 , A23K50/30 , A23K50/75 , A23K50/80 , C07K2319/02 , C12Q1/40 , C12Y302/01001 , Y02A40/818 , Y02P60/877
摘要: The present invention relates to a method for identifying a pepsin resistant alpha amylase enzyme for use in a feed supplement comprising: i) providing an alpha amylase enzyme; ii) admixing said alpha amylase with com based feed and buffer solution comprising a pepsin concentration of 9000 U/ml at pH 3, 40° C., 500 rpm for al least 120 minutes and analysing alpha amylase activity on said alpha amylase compared to a control sample; wherein said control sample differs in that no pepsin is present during incubation; and iii) selecting an alpha amylase enzyme which substantially maintains alpha amylase activity under the assay conditions; feed supplements and feed stuffs comprising a pepsin resistant alpha amylase and the use of pepsin resistant alpha amylases in feed.
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