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1.发明公开Preparation of the penicilline binding protein 2A from staphylococcus aureus 27R, purification and use in a resistant-assay 失效产生从金黄色葡萄球菌27R，纯化和使用中，青霉素结合蛋白2A如在结合测定。
公开(公告)号：EP0505151A2
公开(公告)日：1992-09-23
申请号：EP92302298.2
申请日：1992-03-18
CPC分类号： C12N9/52 , C07K14/31 , C07K2319/00 , C07K2319/02 , C12N15/625
摘要： The invention provides a gene encoding a novel PBP2A penicillin binding protein isolated from the methicillin resistant S. aureus strain 27R. The invention further provides soluble forms of PBP2A proteins lacking the transmembrane association region yet retain their active site configuration of the PBP2A protein. The invention also provides a method of assaying for agents useful against methicillin resistant staphylococcus strains by creating a kinetically inert complex between a support-mobilized transition metal ion and a modified soluble form of the PBP2A protein incorporating a chelating agent. The invention further provides a cell free method of assaying for agents which bind the PBP2A-27R protein.
摘要翻译： 本发明提供了编码来自耐甲氧西林金黄色葡萄球菌菌株27R分离的新颖的PBP2a青霉素结合蛋白的基因。 本发明提供了缺乏跨膜区的关联，但仍保留了蛋白质的PBP2a的其活性位点结构的PBP2a蛋白的进一步可溶形式。 因此本发明提供测定针对通过创建支持动员的过渡金属离子和蛋白质的PBP2a掺入的螯合剂修饰的可溶性形式之间的动力学惰性的复杂耐甲氧西林葡萄球菌菌株有用的试剂的方法。 本发明还提供测定用于结合所述的PBP2a-27R蛋白质剂的无细胞方法。
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公开(公告)号：EP0846769A3
公开(公告)日：1999-11-24
申请号：EP97309702.5
申请日：1997-12-02
发明人： Hoskins, Jo Ann , Norris, Franklin Harpold , Rockey, Pamela Kay , Rosteck, Paul Robert, Jr. , Skatrud, Paul Luther , Treadway, Patti Jean , Bellido, Michele Louise Young , Wu, Chyun-Yeh Earnest
IPC分类号： C12N15/61 , C12N9/90 , C07K14/315 , C12Q1/533
CPC分类号： C12Q1/533 , C12N9/90 , Y10S530/82 , Y10S530/825
摘要： The invention provides isolated nucleic acid compounds encoding the stem peptide biosynthetic gene murI of Streptococcus pneumoniae. Also provided are vectors and transformed heterologous host cells for expressing the MurI enzyme product and a method for identifying compounds that inhibit stem peptide biosynthesis.
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3.发明公开Preparation of the penicilline binding protein 2A from staphylococcus aureus 27R, purification and use in a resistant-assay 失效从番木瓜大蒜素27R中制备PENICILLINE BINDING蛋白2A，在抗性测定中的纯化和使用
公开(公告)号：EP0505151A3
公开(公告)日：1993-04-07
申请号：EP92302298.2
申请日：1992-03-18
CPC分类号： C12N9/52 , C07K14/31 , C07K2319/00 , C07K2319/02 , C12N15/625
摘要： The invention provides a gene encoding a novel PBP2A penicillin binding protein isolated from the methicillin resistant S. aureus strain 27R. The invention further provides soluble forms of PBP2A proteins lacking the transmembrane association region yet retain their active site configuration of the PBP2A protein. The invention also provides a method of assaying for agents useful against methicillin resistant staphylococcus strains by creating a kinetically inert complex between a support-mobilized transition metal ion and a modified soluble form of the PBP2A protein incorporating a chelating agent. The invention further provides a cell free method of assaying for agents which bind the PBP2A-27R protein.
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4.发明公开
公开(公告)号：EP0814163A3
公开(公告)日：1999-10-27
申请号：EP97304281.5
申请日：1997-06-18
CPC分类号： C12N9/93 , C07K2319/00
摘要： The invention provides isolated nucleic acid compounds encoding the murD stem peptide biosynthetic gene of Streptococcus pneumoniae. Also provided are vectors and transformed heterologous host cells for expressing the MurD enzyme product and a method for identifying compounds that inhibit stem peptide biosynthesis.
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公开(公告)号：EP0846769A2
公开(公告)日：1998-06-10
申请号：EP97309702.5
申请日：1997-12-02
发明人： Hoskins, Jo Ann , Norris, Franklin Harpold , Rockey, Pamela Kay , Rosteck, Paul Robert, Jr. , Skatrud, Paul Luther , Treadway, Patti Jean , Bellido, Michele Louise Young , Wu, Chyun-Yeh Earnest
IPC分类号： C12N15/61 , C12N9/90 , C07K14/315 , C12Q1/533
CPC分类号： C12Q1/533 , C12N9/90 , Y10S530/82 , Y10S530/825
摘要： The invention provides isolated nucleic acid compounds encoding the stem peptide biosynthetic gene murI of Streptococcus pneumoniae. Also provided are vectors and transformed heterologous host cells for expressing the MurI enzyme product and a method for identifying compounds that inhibit stem peptide biosynthesis.
摘要翻译： 本发明提供编码肺炎链球菌的干肽生物合成基因murI的分离的核酸化合物。 还提供了用于表达MurI酶产物的载体和转化的异源宿主细胞，以及用于鉴定抑制干肽生物合成的化合物的方法。
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公开(公告)号：EP0229501B1
公开(公告)日：1990-12-27
申请号：EP86309740.8
申请日：1986-12-15
发明人： Rieder, Brent Jeffrey , Spitzer, Wayne Alfred , Wu, Chyun-Yeh Earnest , Paget, Charles Johnson, Jr. , Blanchard, William Bevan
IPC分类号： C12N5/00 , C07D285/12 , A61K31/41
CPC分类号： C07D285/135 , A01N47/36 , A01N47/40 , A01N47/42 , A01N47/44 , A61L2/0088 , A61L2/18
摘要： Compounds of formula (I) wherein: R is hydrogen, and R is cyano, or -C(=S)-NH-R ; or R and R taken together are =C(NH2)2 or =C(R )(NHR min ); R and R min are hydrogen or -COOR ; R is C1-C10 alkyl, substituted C1 -C10 alkyl, C2-C10 alkenyl, substituted C2-C10 alkenyl, phenyl, or substituted phenyl; and R is hydrogen or -S-R where R is C1-C4 alkyl, cyano-C1-C4 alkyl or pyridyl-C1-C4 alkyl, and salts thereof, are useful antiviral agents.
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公开(公告)号：EP0229501A1
公开(公告)日：1987-07-22
申请号：EP86309740.8
申请日：1986-12-15
发明人： Rieder, Brent Jeffrey , Spitzer, Wayne Alfred , Wu, Chyun-Yeh Earnest , Paget, Charles Johnson, Jr. , Blanchard, William Bevan
IPC分类号： C12N5/00 , C07D285/12 , A61K31/41
CPC分类号： C07D285/135 , A01N47/36 , A01N47/40 , A01N47/42 , A01N47/44 , A61L2/0088 , A61L2/18
摘要： Compounds of formula (I)
wherein:
R' is hydrogen, and R 2 is cyano, or -C(=S)-NH-R 3 ; or
R' and R 2 taken together are = C(NH 2 ) 2 or =C(R 5 )(NHR 3 ');
R 3 and R 3 ' are hydrogen or -COOR';
R 4 is C 1 -C 10 alkyl, substituted C, -C 10 alkyl, C 2 -C 10 alkenyl, substituted C 2 -C 10 alkenyl, phenyl, or substituted phenyl; and
R 5 is hydrogen or -S-R 6 where R 6 is C,-C 4 alkyl, cyano-C 1 -C 4 alkyl or pyridyl-C 1 -C 4 alkyl, and salts thereof, are useful antiviral agents.摘要翻译： 式（I）化合物
其中：R 1是氢，R 2是氰基或-C（= S）-NH-R 3; 或R 1和R 2一起为= C（NH 2）2或= C（R 5）（NHR 3min）; R 3和R 3min是氢或-COOR 4; R 4是C 1 -C 10烷基，取代的C 1 -C 10烷基，C 2 -C 10烯基，取代的C 2 -C 10烯基，苯基或取代的苯基; R 5是氢或-S-R 6，其中R 6是C 1 -C 4烷基，氰基-C 1 -C 4烷基或吡啶基-C 1 -C 4烷基，及其盐是有用的抗病毒剂。
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