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公开(公告)号：EP0280460A2
公开(公告)日：1988-08-31
申请号：EP88301284.1
申请日：1988-02-17
CPC分类号： C07K14/65 , A61K38/00 , A61L26/0066 , A61L26/008 , A61L2300/414 , Y02A50/473
摘要： There is described an invention for promoting the rate and improving the quality of wound healing by topically applying a pharmaceutical formulation comprising insulin-like growth factor-II.
摘要翻译： 描述了通过局部施用包含胰岛素样生长因子-II的药物制剂来促进速率并提高伤口愈合质量的发明。
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公开(公告)号：EP0184355A2
公开(公告)日：1986-06-11
申请号：EP85308471.3
申请日：1985-11-21
CPC分类号： C12N15/62 , B01J20/223 , B01J20/286 , B01J20/3212 , B01J20/3265 , B01J2220/49 , B01J2220/54 , B01J2220/58 , C07K1/22 , C07K2319/00 , C07K2319/20 , C07K2319/50
摘要： This invention describes a process for separating a biologically active polypeptide or protein in the form of its precursor from a mixture containing said precursor and impurities, which comprises contacting said precursor with a resin containing immobilized metal ions, said precursor comprising the biologically active polypeptide or protein covalently linked directly or indirectly to an immobilized metal ion chelating peptide, binding said precursor to said resin, and selectively eluting said precursor from said resin. The corresponding precursor compounds also are described.
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3.发明公开Preparation of the penicilline binding protein 2A from staphylococcus aureus 27R, purification and use in a resistant-assay 失效产生从金黄色葡萄球菌27R，纯化和使用中，青霉素结合蛋白2A如在结合测定。
公开(公告)号：EP0505151A2
公开(公告)日：1992-09-23
申请号：EP92302298.2
申请日：1992-03-18
CPC分类号： C12N9/52 , C07K14/31 , C07K2319/00 , C07K2319/02 , C12N15/625
摘要： The invention provides a gene encoding a novel PBP2A penicillin binding protein isolated from the methicillin resistant S. aureus strain 27R. The invention further provides soluble forms of PBP2A proteins lacking the transmembrane association region yet retain their active site configuration of the PBP2A protein. The invention also provides a method of assaying for agents useful against methicillin resistant staphylococcus strains by creating a kinetically inert complex between a support-mobilized transition metal ion and a modified soluble form of the PBP2A protein incorporating a chelating agent. The invention further provides a cell free method of assaying for agents which bind the PBP2A-27R protein.
摘要翻译： 本发明提供了编码来自耐甲氧西林金黄色葡萄球菌菌株27R分离的新颖的PBP2a青霉素结合蛋白的基因。 本发明提供了缺乏跨膜区的关联，但仍保留了蛋白质的PBP2a的其活性位点结构的PBP2a蛋白的进一步可溶形式。 因此本发明提供测定针对通过创建支持动员的过渡金属离子和蛋白质的PBP2a掺入的螯合剂修饰的可溶性形式之间的动力学惰性的复杂耐甲氧西林葡萄球菌菌株有用的试剂的方法。 本发明还提供测定用于结合所述的PBP2a-27R蛋白质剂的无细胞方法。
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4.发明公开Penicllin binding protein 2A from staphylococcus aureus strain 27R and derivatives for use in purification therefor and assay for compounds effective against methicillin resistant organisms 失效青霉素的衍生物从金黄色葡萄球菌菌株27R结合蛋白2A用于其纯化并测定耐甲氧西林的微生物有效化合物的
公开(公告)号：EP0875578A3
公开(公告)日：1999-05-12
申请号：EP98200530.8
申请日：1992-03-18
CPC分类号： C12N9/52 , C07K14/31 , C07K2319/00 , C07K2319/02 , C12N15/625
摘要： The invention provides a gene encoding a novel PBP2A penicillin binding protein isolated from the methicillin resistant S . aureus strain 27R. The invention further provides soluble forms of PBP2A proteins lacking the transmembrane association region yet retain their active site configuration of the PBP2A protein. The invention also provides a method of assaying for agents useful against methicillin resistant staphylococcus strains by creating a kinetically inert complex between a support-immobilized transition metal ion and a modified soluble form of the PBP2A protein incorporating a chelating agent. The invention further provides a cell free method of assaying for agents which bind the PBP2A-27R protein.
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5.发明公开Preparation of the penicilline binding protein 2A from staphylococcus aureus 27R, purification and use in a resistant-assay 失效从番木瓜大蒜素27R中制备PENICILLINE BINDING蛋白2A，在抗性测定中的纯化和使用
公开(公告)号：EP0505151A3
公开(公告)日：1993-04-07
申请号：EP92302298.2
申请日：1992-03-18
CPC分类号： C12N9/52 , C07K14/31 , C07K2319/00 , C07K2319/02 , C12N15/625
摘要： The invention provides a gene encoding a novel PBP2A penicillin binding protein isolated from the methicillin resistant S. aureus strain 27R. The invention further provides soluble forms of PBP2A proteins lacking the transmembrane association region yet retain their active site configuration of the PBP2A protein. The invention also provides a method of assaying for agents useful against methicillin resistant staphylococcus strains by creating a kinetically inert complex between a support-mobilized transition metal ion and a modified soluble form of the PBP2A protein incorporating a chelating agent. The invention further provides a cell free method of assaying for agents which bind the PBP2A-27R protein.
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公开(公告)号：EP0280460A3
公开(公告)日：1990-06-06
申请号：EP88301284.1
申请日：1988-02-17
CPC分类号： C07K14/65 , A61K38/00 , A61L26/0066 , A61L26/008 , A61L2300/414 , Y02A50/473
摘要： There is described an invention for promoting the rate and improving the quality of wound healing by topically applying a pharmaceutical formulation comprising insulin-like growth factor-II.
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7.发明公开Penicllin binding protein 2A from staphylococcus aureus strain 27R and derivatives for use in purification therefor and assay for compounds effective against methicillin resistant organisms 失效青霉素的衍生物从金黄色葡萄球菌菌株27R结合蛋白2A用于其纯化并测定耐甲氧西林的微生物有效化合物的
公开(公告)号：EP0875578A2
公开(公告)日：1998-11-04
申请号：EP98200530.8
申请日：1992-03-18
CPC分类号： C12N9/52 , C07K14/31 , C07K2319/00 , C07K2319/02 , C12N15/625
摘要： The invention provides a gene encoding a novel PBP2A penicillin binding protein isolated from the methicillin resistant S . aureus strain 27R. The invention further provides soluble forms of PBP2A proteins lacking the transmembrane association region yet retain their active site configuration of the PBP2A protein. The invention also provides a method of assaying for agents useful against methicillin resistant staphylococcus strains by creating a kinetically inert complex between a support-immobilized transition metal ion and a modified soluble form of the PBP2A protein incorporating a chelating agent. The invention further provides a cell free method of assaying for agents which bind the PBP2A-27R protein.
摘要翻译： 本发明提供了编码来自耐甲氧西林金黄色葡萄球菌菌株27R分离的新颖的PBP2a青霉素结合蛋白的基因。 本发明提供了缺乏跨膜区的关联，但仍保留了蛋白质的PBP2a的其活性位点结构的PBP2a蛋白的进一步可溶形式。 因此本发明提供测定针对通过创建一个支撑固定化的过渡金属离子和蛋白质的PBP2a掺入的螯合剂修饰的可溶性形式之间的动力学惰性的复杂耐甲氧西林葡萄球菌菌株有用的试剂的方法。 本发明还提供测定用于结合所述的PBP2a-27R蛋白质剂的无细胞方法。
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公开(公告)号：EP0184355B1
公开(公告)日：1992-01-08
申请号：EP85308471.3
申请日：1985-11-21
CPC分类号： C12N15/62 , B01J20/223 , B01J20/286 , B01J20/3212 , B01J20/3265 , B01J2220/49 , B01J2220/54 , B01J2220/58 , C07K1/22 , C07K2319/00 , C07K2319/20 , C07K2319/50
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公开(公告)号：EP0184355A3
公开(公告)日：1987-04-08
申请号：EP85308471
申请日：1985-11-21
CPC分类号： C12N15/62 , B01J20/223 , B01J20/286 , B01J20/3212 , B01J20/3265 , B01J2220/49 , B01J2220/54 , B01J2220/58 , C07K1/22 , C07K2319/00 , C07K2319/20 , C07K2319/50
摘要： This invention describes a process for separating a biologically active polypeptide or protein in the form of its precursor from a mixture containing said precursor and impurities, which comprises contacting said precursor with a resin containing immobilized metal ions, said precursor comprising the biologically active polypeptide or protein covalently linked directly or indirectly to an immobilized metal ion chelating peptide, binding said precursor to said resin, and selectively eluting said precursor from said resin. The corresponding precursor compounds also are described.
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