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    INTACT MINICELLS AS VECTORS FOR DNA TRANSFER AND GENE THERAPY IN VITRO AND IN VIVO
    1.
    发明授权
    INTACT MINICELLS AS VECTORS FOR DNA TRANSFER AND GENE THERAPY IN VITRO AND IN VIVO 有权
    原状MINI细胞的基因治疗和DNA转移载体在体外和体内

    公开(公告)号：EP1446489B1

    公开(公告)日：2012-01-18

    申请号：EP02783361.5

    申请日：2002-10-15

    申请人： EnGeneIC Molecular Delivery Pty Ltd.

    发明人： BRAHMBHATT, Himanshu ,  MACDIARMID, Jennifer

    IPC分类号： C12N15/85

    CPC分类号： C12N15/87 ,  A61K48/0008 ,  A61K2039/52

    摘要： A composition comprising recombinant, intact minicells that contain a therapeutic nucleic acid molecule is disclosed. Methods for purifying a preparation of such minicells also are disclosed. Additionally, a genetic transformation method is disclosed, which comprises (i) making recombinant, intact minicells available that contain a plasmid comprised of a first nucleic acid segment, and (ii) bringing the minicells into contact with mammalian cells that are engulfing-competent, such that the minicells are engulfed by the mammalian cells, which thereafter produce an expression product of the first nucleic acid segment.

    PHARMACEUTICALLY COMPATIBLE METHOD FOR PURIFYING INTACT BACTERIAL MINICELLS
    8.
    发明授权
    PHARMACEUTICALLY COMPATIBLE METHOD FOR PURIFYING INTACT BACTERIAL MINICELLS 有权
    用于清洁完整的细菌细胞MINI药学上可接受的方法

    公开(公告)号：EP1636344B1

    公开(公告)日：2010-03-17

    申请号：EP04743793.4

    申请日：2004-06-23

    申请人： EnGeneIC Molecular Delivery Pty Ltd.

    发明人： BRAHMBHATT, Himanshu ,  MACDIARMID, Jennifer

    IPC分类号： C12N1/02

    CPC分类号： A61K35/74 ,  C12N1/02 ,  C12N1/20

    摘要： The present invention provides a method for purifying bacterial minicells that involves subjecting a sample containing minicells to density gradient centrifugation in a biologically compatible medium. The method optionally includes a preliminary differential centrifugation step and one or more filtration steps. The invention also provides a method for purifying bacterial minicells in which a sample containing minicells is subjected to a condition that induces parent bacterial cells to adopt a filamentous form, followed by filtration of the sample to separate minicells from parent bacterial cells. The inventive methods optionally include one or more steps to remove endotoxin from purified minicell preparations, and/or treatment of purified minicell preparations with an antibiotic. Additionally, the invention provides purified minicell preparations, prepared according to the foregoing methods, and containing fewer than about 1 contaminating parent bacterial cell per 107, 108, 109, 1010, or 1011 minicells.

    PHARMACEUTICALLY COMPATIBLE METHOD FOR PURIFYING INTACT BACTERIAL MINICELLS
    9.
    发明公开
    PHARMACEUTICALLY COMPATIBLE METHOD FOR PURIFYING INTACT BACTERIAL MINICELLS 有权
    用于清洁完整的细菌细胞MINI药学上可接受的方法

    公开(公告)号：EP1636344A1

    公开(公告)日：2006-03-22

    申请号：EP04743793.4

    申请日：2004-06-23

    申请人： EnGeneIC Molecular Delivery Pty Ltd.

    发明人： BRAHMBHATT, Himanshu ,  MACDIARMID, Jennifer

    IPC分类号： C12N1/02

    CPC分类号： A61K35/74 ,  C12N1/02 ,  C12N1/20

    摘要： The present invention provides a method for purifying bacterial minicells that involves subjecting a sample containing minicells to density gradient centrifugation in a biologically compatible medium. The method optionally includes a preliminary differential centrifugation step and one or more filtration steps. The invention also provides a method for purifying bacterial minicells in which a sample containing minicells is subjected to a condition that induces parent bacterial cells to adopt a filamentous form, followed by filtration of the sample to separate minicells from parent bacterial cells. The inventive methods optionally include one or more steps to remove endotoxin from purified minicell preparations, and/or treatment of purified minicell preparations with an antibiotic. Additionally, the invention provides purified minicell preparations, prepared according to the foregoing methods, and containing fewer than about 1 contaminating parent bacterial cell per 107, 108, 109, 1010, or 1011 minicells.