摘要:
The present invention discloses a method for fusing or contacting reactor and reagent droplets in a three-phase microfluidic or millifluidic system with one of the phases being continuous, in which the reagent droplet is injected in the droplet train before it coalesces or contacts with the reactor droplet. More specifically, the reagent droplet detaches from the reagent inlet before being in contact with the reactor droplet. The method of the invention involves two successive but not concomitant steps: first producing in the droplet train a droplet of a reagent which breaks-up from the reagent reservoir before the second step of merging. The invention also discloses a milli/microfluidic device whose equivalent circuit is represented on FIG. 11.
摘要:
The present invention relates to a novel method for cultivating micro-organisms by confinement in micro-bioreactors. Said method comprises using a capillary tube in which a carrier fluid for moving a train of droplets forward flows, said capillary tube comprising micro-bioreactors in which the culture of said micro-organisms takes place, wherein said micro-bioreactors are separated by a spacing fluid which is a gas. The diameter of the micro-bioreactors (5) is smaller than that of the capillary tube (8) and the size of the bubble (6) of said spacing fluid is within a range of two to ten times the diameter of said capillary tube (8). The method can be used for cultivating micro-organisms such as thread-like fungi or planktonic algae.
摘要:
The invention relates to a method for recuperating droplets (4), comprising the following steps: - supplying a chip (20) comprising a fluid circulation duct (46), - injecting an emulsion (6) of droplets, - injecting a carrier fluid (26) to form a working fluid (28) containing droplets of the emulsion which are spaced apart along the duct, - conveying the working fluid through the duct, - injecting a separating fluid (33) in order to separate the working fluid into a plurality of successive pockets (35), each pocket being isolated from the next pocket by a separator (80), - conveying the pockets and separators towards an outlet (66) of the chip, - recuperating in a compartment (82) of a recuperation support (34) at least one pocket containing a droplet recuperated after conveying.
摘要:
The present invention relates to a method for manipulating the evolution of collectives of self-replicating entities and/or variation between collectives of self-replicating entities, in a high throughput droplet milli-fluidic system.
摘要:
La présente invention concerne un procédé de lecture d'une émulsion (3) comprenant des gouttes et une phase continue entourant les gouttes, ledit procédé comprenant : - un balayage bidimensionnel de l'émulsion (3), et - une construction d'une image bidimensionnelle de l'émulsion (3) à partir dudit balayage. De préférence, les gouttes ne bougent pas pendant le balayage, par exemple en solidifiant la phase continue ou en utilisant un réseau bidimensionnel compact ou semi compact de gouttes. Le procédé selon l'invention peut comprendre en outre un suivi dans le temps d'une réaction chimique ou biologique se déroulant dans au moins une des gouttes. L'invention concerne aussi un dispositif mettant en œuvre ce procédé. Application à la détection et/ou le tri de microgouttes jouant le rôle de microréacteurs ou contenant des cellules ou des molécules spécifiques, dans des domaines tels que l'expression de gènes ou le diagnostic.