A METHOD FOR FUSING OR CONTACTING REACTOR AND REAGENT DROPLETS IN A MICROFLUIDIC OR MILLIFLUIDIC DEVICE
    6.
    发明公开
    A METHOD FOR FUSING OR CONTACTING REACTOR AND REAGENT DROPLETS IN A MICROFLUIDIC OR MILLIFLUIDIC DEVICE 有权
    程序对在定影器或KONTAKTIERUNGSREAKTOR及试剂滴在微流体或MILLIFLUIDISCHEN DEVICE

    公开(公告)号:EP3177401A1

    公开(公告)日:2017-06-14

    申请号:EP15744611.3

    申请日:2015-08-05

    IPC分类号: B01L3/00

    摘要: The present invention discloses a method for fusing or contacting reactor and reagent droplets in a three-phase microfluidic or millifluidic system with one of the phases being continuous, in which the reagent droplet is injected in the droplet train before it coalesces or contacts with the reactor droplet. More specifically, the reagent droplet detaches from the reagent inlet before being in contact with the reactor droplet. The method of the invention involves two successive but not concomitant steps: first producing in the droplet train a droplet of a reagent which breaks-up from the reagent reservoir before the second step of merging. The invention also discloses a milli/microfluidic device whose equivalent circuit is represented on FIG. 11.

    摘要翻译: 本发明盘松的方法用于融合或与一相是连续的,其中聚结或接触反应器前将试剂液滴被喷射到液滴列车接触型反应器和试剂液滴中的三相微流体或millifluidic系统 滴。 更具体而言,试剂液滴从试剂入口与所述反应器的液滴接触之前分离。 本发明的方法包括两个连续的但不伴随步骤:在液滴首先生产培养试剂打破时从试剂贮存合并的第二步骤之前的液滴。 因此,本发明光盘松毫/微流体装置是谁的等效电路被表示于图11中

    NOUVEAU PROCÉDÉ POUR LA CULTURE DE MICROORGANISMES PAR CONFINEMENT DANS DES MICRO-BIORÉACTEURS
    7.
    发明公开
    NOUVEAU PROCÉDÉ POUR LA CULTURE DE MICROORGANISMES PAR CONFINEMENT DANS DES MICRO-BIORÉACTEURS 有权
    中华人民共和国维也纳保加利亚革命组织

    公开(公告)号:EP3019590A1

    公开(公告)日:2016-05-18

    申请号:EP14739774.9

    申请日:2014-07-10

    IPC分类号: C12M3/06 C12M1/12

    CPC分类号: C12M23/16 C12M25/01

    摘要: The present invention relates to a novel method for cultivating micro-organisms by confinement in micro-bioreactors. Said method comprises using a capillary tube in which a carrier fluid for moving a train of droplets forward flows, said capillary tube comprising micro-bioreactors in which the culture of said micro-organisms takes place, wherein said micro-bioreactors are separated by a spacing fluid which is a gas. The diameter of the micro-bioreactors (5) is smaller than that of the capillary tube (8) and the size of the bubble (6) of said spacing fluid is within a range of two to ten times the diameter of said capillary tube (8). The method can be used for cultivating micro-organisms such as thread-like fungi or planktonic algae.

    摘要翻译: 本发明涉及通过限制在微生物反应器中培养微生物的新方法。 所述方法包括使用毛细管,其中用于将一串液滴向前移动的载体流体流动,所述毛细管包括其中发生所述微生物的培养的微生物反应器,其中所述微生物反应器被间隔开 作为气体的流体。 微生物反应器(5)的直径小于毛细管(8)的直径,并且所述间隔流体的气泡(6)的尺寸在所述毛细管的直径的2至10倍的范围内 8)。 该方法可用于培养微生物如线状真菌或浮游藻类。

    PARTICULES LIQUIDES BIOMIMÉTIQUES, PROCÉDÉ ET DISPOSITIF DE MESURE EN CYTOMÉTRIE EN FLUX
    10.
    发明公开
    PARTICULES LIQUIDES BIOMIMÉTIQUES, PROCÉDÉ ET DISPOSITIF DE MESURE EN CYTOMÉTRIE EN FLUX 审中-公开
    PARTICULES LIQUIDESBIOMIMÉTIQUES,PROCÉDÉET DISPOSITIF DE MESURE ENCYTOMÉTRIEEN FLUX

    公开(公告)号:EP3158316A1

    公开(公告)日:2017-04-26

    申请号:EP15728576.8

    申请日:2015-06-16

    IPC分类号: G01N15/10 G01N15/14 G01N15/00

    摘要: The present invention relates to the use of a solution of liquid particles in suspension for the control, calibration and/or performance of physical, in particular optical, measurements in a flow cytometry device for analysis of biological cells, said solution comprising liquid particles (21) of a first liquid phase dispersed in a second liquid phase, said liquid particles (21) having physical, chemical and/or biochemical properties that enable the attainment of physical measurements in said flow cytometry device similar to measurements obtained with biological cells, and said liquid particles (21), having a diameter for which the coefficient of variation within the solution of liquid particles in suspension is less than 10%. The invention also relates to a measurement method and a flow cytometry device implementing the solution of liquid particles in suspension.

    摘要翻译: 本发明涉及悬浮液中的液体颗粒溶液用于流式细胞仪分析生物细胞的物理特别是光学测量的控制,校准和/或性能的用途,所述溶液包含液体颗粒(21 )分散在第二液相中的第一液相,所述液体颗粒(21)具有物理,化学和/或生物化学性质,所述物理,化学和/或生物化学性质使得能够在所述流式细胞术装置中获得与用生物细胞获得的测量相似的物理测量结果,并且所述 液体颗粒(21)具有直径,液体颗粒悬浮液溶液内的变化系数小于10%。 本发明还涉及实施悬浮液中液体颗粒溶液的测量方法和流式细胞仪。