摘要:
The invention relates to a method for recuperating droplets (4), comprising the following steps: - supplying a chip (20) comprising a fluid circulation duct (46), - injecting an emulsion (6) of droplets, - injecting a carrier fluid (26) to form a working fluid (28) containing droplets of the emulsion which are spaced apart along the duct, - conveying the working fluid through the duct, - injecting a separating fluid (33) in order to separate the working fluid into a plurality of successive pockets (35), each pocket being isolated from the next pocket by a separator (80), - conveying the pockets and separators towards an outlet (66) of the chip, - recuperating in a compartment (82) of a recuperation support (34) at least one pocket containing a droplet recuperated after conveying.
摘要:
The present invention discloses a method for fusing or contacting reactor and reagent droplets in a three-phase microfluidic or millifluidic system with one of the phases being continuous, in which the reagent droplet is injected in the droplet train before it coalesces or contacts with the reactor droplet. More specifically, the reagent droplet detaches from the reagent inlet before being in contact with the reactor droplet. The method of the invention involves two successive but not concomitant steps: first producing in the droplet train a droplet of a reagent which breaks-up from the reagent reservoir before the second step of merging. The invention also discloses a milli/microfluidic device whose equivalent circuit is represented on FIG. 11.
摘要:
The present invention relates to a novel method for cultivating micro-organisms by confinement in micro-bioreactors. Said method comprises using a capillary tube in which a carrier fluid for moving a train of droplets forward flows, said capillary tube comprising micro-bioreactors in which the culture of said micro-organisms takes place, wherein said micro-bioreactors are separated by a spacing fluid which is a gas. The diameter of the micro-bioreactors (5) is smaller than that of the capillary tube (8) and the size of the bubble (6) of said spacing fluid is within a range of two to ten times the diameter of said capillary tube (8). The method can be used for cultivating micro-organisms such as thread-like fungi or planktonic algae.
摘要:
The present invention concerns a capsule comprising at least one cell with blood-forming potential, said capsule being formed of a liquid core and at least one gelled shell completely encapsulating the liquid core at the periphery of same, the use of such a capsule to produce enucleated erythroid cells ex vivo , as well as an ex vivo method of producing enucleated erythroid cells using said capsule.
摘要:
The present invention relates to the use of a solution of liquid particles in suspension for the control, calibration and/or performance of physical, in particular optical, measurements in a flow cytometry device for analysis of biological cells, said solution comprising liquid particles (21) of a first liquid phase dispersed in a second liquid phase, said liquid particles (21) having physical, chemical and/or biochemical properties that enable the attainment of physical measurements in said flow cytometry device similar to measurements obtained with biological cells, and said liquid particles (21), having a diameter for which the coefficient of variation within the solution of liquid particles in suspension is less than 10%. The invention also relates to a measurement method and a flow cytometry device implementing the solution of liquid particles in suspension.