公开(公告)号：EP1356037B1

公开(公告)日：2011-03-09

申请号：EP02709979.5

申请日：2002-01-25

申请人： Evolva Ltd.

发明人： GOLDSMITH, Neil ,  SORENSEN, Alexandra, M., P., SantAna ,  NIELSEN, Soren, V., S.

IPC分类号： C12N15/10

CPC分类号： C12N15/1034 ,  C12N15/1086 ,  C12N15/66

摘要： The present invention relates to combinatorial gene expression libraries and methods for making these. The library comprises a collection of individual cells, the cells being denoted celll,cell2..., cell, wherein i > 2, each cell comprising at least one concatemer of individual oligonucleotide cassettes, each concatemer comprising a nucleotide sequence of the following formula: (rs2-SP-PR-X-TR-SP-rs1) n wherein rsl and rs2 together denote arestriction site, SP denotes aspacerof at least two bases, X denotes anexpressible nucleotide sequence,PRdenotes a promoter, capable of regulatingthe expression of X in the cell,TRdenotes a terminator, and n 2, and whereinat least one concatemer of celllisdifferent from a concatemer cell2.Such libraries are useful in discovery of novel and/or enhanced metabolic pathways leading to the production of novel compounds for e.g. drug discovery and/or to the production of known compounds in novel quantities or in novel compartments of the cells. The expression libraries in particular are composed of host cells capable of co-ordinated and controllable expression of large numbers of heterologous gene in the host cells. co-ordinated and controllable expression of large numbers of heterologous gene i host cells.

公开(公告)号：EP1360308B1

公开(公告)日：2007-10-10

申请号：EP02709978.7

申请日：2002-01-25

申请人： Evolva Ltd.

发明人： GOLDSMITH, Neil ,  SORENSEN, Alexandra, M., P., SantAna ,  NIELSEN, Soren, V., S. ,  NAESBY, Michael

IPC分类号： C12N15/66 ,  C12N15/10

CPC分类号： C12N15/81 ,  C12N15/66 ,  C12N15/70 ,  C12N15/85 ,  C12N2800/20 ,  C12N2800/206

摘要： In the present invention are disclosed concatemers of concatenated expression cassettes and vectors that enable the synthesis of such concatemers. The main purpose of these concatemers is the controllable and co-ordinated expression of large numbers of heterologous genes in a single host. Furthermore, the invention relates to a concatemers of cassettes of nucleotide sequences and a method for preparing the concatemers. In a further aspect, the invention relates to transgenic host cells comprising at least one concatemer according to the invention, as well as to a method for preparatin the transgenic host cells. Finally, the invention relates to a vector comprising a cassette of nucleotides, a method for preparing said vector, a nucleotide library comprising at least two primary vectors each comprising a cassette of nucleotides, a method for prepararing the library.