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    METHOD FOR THE DETERMINATION OF SEQUENCE VARIANTS OF POLYPEPTIDES
    1.
    发明公开
    METHOD FOR THE DETERMINATION OF SEQUENCE VARIANTS OF POLYPEPTIDES 有权
    多肽的一种用于确定序列的方法的变体

    公开(公告)号：EP2537035A1

    公开(公告)日：2012-12-26

    申请号：EP11704217.6

    申请日：2011-02-16

    申请人： F.Hoffmann-La Roche AG

    发明人： KOLL, Hans ,  REGULA, Joerg Thomas ,  WIEGESHOFF, Frank ,  ZECK, Anne

    IPC分类号： G01N33/68

    CPC分类号： C12Q1/37 ,  G01N33/6848 ,  G01N2560/00

    摘要： The current invention is directed to a method for determining amino acid sequence mutations in a produced polypeptide, comprising the following steps of a) providing a sample of a produced polypeptide, b) incubating the polypeptide in the sample with a protease, c) performing a two dimensional analysis using reversed phase chromatography coupled with a high resolution mass spectroscopy (FT-ICR / FT-orbitrap) and MS/MS analysis of the amino acid sequence fragments of the peptides, d) data evaluation by comparing the LC-MS data sets obtained for the samples side by side with the data set of a reference sample, by searching for differences in the signal intensities at given retention times and by evaluation of differential signals with respect to amino acid sequence mutations. The reference sample for data evaluation (d) can be either a well characterized standard or one of the samples to be analyzed.

    FC-RECEPTOR BINDING MODIFIED ASYMMETRIC ANTIBODIES AND METHODS OF USE
    5.
    发明公开
    FC-RECEPTOR BINDING MODIFIED ASYMMETRIC ANTIBODIES AND METHODS OF USE 审中-公开
    FC-REZEPTOR-BINDENDE MODIFIZIERTE不对称防盗器VERFAHREN ZUR VERWENDUNG

    公开(公告)号：EP2992010A2

    公开(公告)日：2016-03-09

    申请号：EP14719743.8

    申请日：2014-04-25

    申请人： F.Hoffmann-La Roche AG

    发明人： REGULA, Joerg Thomas ,  SCHAEFER, Wolfgang ,  SCHLOTHAUER, Tilman

    IPC分类号： C07K16/22 ,  C07K16/46 ,  A61K39/395 ,  A61P27/02 ,  C07K16/28

    CPC分类号： C07K16/22 ,  A61K2039/505 ,  A61K2039/54 ,  C07K16/00 ,  C07K16/2863 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/35 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/71 ,  C07K2317/92 ,  C07K2317/94

    摘要： Herein is reported an IgG class Fc-region comprising a first variant Fc-region polypeptide and a second variant Fc-region polypeptide, wherein the first variant Fc-region polypeptide is derived from a first parent IgG class Fc-region polypeptide and the second variant Fc-region polypeptide is derived from a second parent IgG class Fc-region polypeptide, whereby the first parent IgG class Fc-region polypeptide is identical to or different from the second parent IgG class Fc-region polypeptide, and the first variant Fc-region polypeptide differs from the second variant Fc-region polypeptide in one or more amino acid residues other than those amino acid residues in which the first parent IgG class Fc-region polypeptide differs from the second parent IgG class Fc-region polypeptide, and the IgG class Fc-region comprising the first variant Fc-region polypeptide and the second variant Fc-region polypeptide has an affinity to a human Fc-receptor that is different than that of an IgG class Fc-region comprising the first parent IgG class Fc-region polypeptide of a) and the second parent IgG class Fc-region polypeptide of a).

    摘要翻译： 本文报道了包含第一变体Fc区多肽和第二变体Fc区多肽的IgG类Fc区，其中第一变体Fc区多肽衍生自第一亲本IgG类Fc区多肽，第二变体 Fc区多肽衍生自第二亲本IgG类Fc区多肽，由此第一亲本IgG类Fc区多肽与第二亲本IgG类Fc区多肽相同或不同，第一变体Fc区 多肽与第一亲本IgG类Fc区多肽与第二亲本IgG类Fc区多肽不同的那些氨基酸残基之外的一个或多个氨基酸残基中的第二变体Fc区多肽不同，IgG类 包含第一变体Fc区多肽和第二变体Fc区多肽的Fc区与人Fc受体具有不同于IgG类Fc区多肽的亲和力， a）的第一亲本IgG类Fc区多肽和a）的第二亲本IgG类Fc区多肽。