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公开(公告)号:EP3234099A1
公开(公告)日:2017-10-25
申请号:EP15825713.9
申请日:2015-12-18
发明人: LIPKENS, Bart , MILLER, Erik , ROSS-JOHNSRUD, Benjamin , PRESZ, Walter M. Jr. , CHITALE, Kedar , KENNEDY, Thomas J. III
摘要: Acoustic perfusion devices for separating biological cells from other material in a fluid medium are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid medium is being drawn.
摘要翻译: 公开了用于从流体介质中的其他材料分离生物细胞的声灌注装置。 这些装置包括连接到声室的入口端口,出口端口和收集端口。 超声换能器在声室中产生声驻波,其允许流体的连续流动通过采集端口被回收,同时保持声室内的生物细胞返回到流体介质从其中被抽出的生物反应器。
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公开(公告)号:EP3762482A1
公开(公告)日:2021-01-13
申请号:EP19764743.1
申请日:2019-03-08
发明人: LIPKENS, Bart , TOSTOES, Rui , DUTRA, Brian , GILMANSHIN, Rudolf , DIONNE, Jason , PRESZ, Walter, M., Jr. , ROSS-JOHNSRUD, Benjamin , GHOSHAL, Goutam , CHITALE, Kedar
IPC分类号: C12N5/0783 , C12N5/00 , C12N13/00 , C12M1/00
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公开(公告)号:EP3234099B1
公开(公告)日:2020-06-03
申请号:EP15825713.9
申请日:2015-12-18
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公开(公告)号:EP3341463A1
公开(公告)日:2018-07-04
申请号:EP16725630.4
申请日:2016-04-26
发明人: LIPKENS, Bart , MILLER, Erik , ROSS-JOHNSRUD, Benjamin , PRESZ, JR., Walter M. , CHITALE, Kedar , KENNEDY, III, Thomas J. , WINIARSKI, Lauryn
CPC分类号: C12M29/10 , B01D21/283 , C12M33/00 , C12M47/02 , C12M47/10
摘要: Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.
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公开(公告)号:EP3164488A2
公开(公告)日:2017-05-10
申请号:EP15814616.7
申请日:2015-07-02
发明人: LIPKENS, Bart , MCCARTHY, Brian , BARNES, Jason , MEALEY, Dane , ROSS-JOHNSRUD, Ben , PRESZ JR, Walter M. , CHITALE, Kedar
IPC分类号: C12N13/00
CPC分类号: B01D21/283 , B01D21/0045
摘要: An acoustophoresis device which includes a substantially vertical flow path of the fluid mixture in order to improve separation of particles/secondary fluid from a primary fluid is disclosed. The vertical flow path reduces velocity non-uniformities in the acoustic chamber resulting from gravity forces. The device includes an acoustic chamber in which multidimensional acoustic standing waves are generated. The fluid can be introduced into the acoustic chamber using a dump diffuser in which a plurality of inlets enter near the bottom of the acoustic chamber such that flow symmetry reduces both, gravity driven flow non-uniformities, and any flow interference effects between inlet mixture flow into the acoustic chamber and the continuous gravity driven particle cluster drop out.
摘要翻译: 公开了包括流体混合物的基本上垂直的流动路径以便改善颗粒/次要流体与主要流体的分离的声波输送装置。 垂直流动路径减小了由重力引起的声室中的速度不均匀性。 该装置包括其中产生多维声驻波的声室。 可以使用倾倒扩散器将流体引入声学腔室,其中多个入口进入声学腔室的底部附近,使得流动对称性降低重力驱动的流动不均匀性和入口混合物流动之间的任何流动干涉效应 进入声室并且连续重力驱动的粒子群落退出。
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公开(公告)号:EP3341102A1
公开(公告)日:2018-07-04
申请号:EP16770116.8
申请日:2016-08-26
发明人: LIPKENS, Bart , PRESZ JR, Walter M. , KING, Jeffrey , BARNES, Jason , MEALEY, Dane , MCCARTHY, Brian , ROSS-JOHNSRUD, Benjamin , CHITALE, Kedar
CPC分类号: B01D21/283 , A61M1/3678 , B06B1/0622 , G10K11/28 , G10K11/34 , G10K15/00
摘要: Devices (100) for separating a host fluid from a second fluid or particulate are disclosed. The devices include an acoustic chamber (107), a fluid outlet at a top end of the acoustic chamber (114), a concentrate outlet at a bottom end of the acoustic chamber (116), and an inlet (112) on a first side end (122) of the acoustic chamber (107). An ultrasonic transducer (106) on a side wall of the acoustic chamber and reflector at the opposite side wall create a multi-dimensional acoustic standing wave in the acoustic chamber (107) that traps and separates particulates (e.g. cells) from a host fluid. The host fluid is collected via the fluid outlet (114), and the particulates are collected via the concentrate outlet (116). The device is a large-scale device that is able to process liters/hour, and has a large interior volume.
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公开(公告)号:EP3288660A1
公开(公告)日:2018-03-07
申请号:EP16732365.8
申请日:2016-04-29
发明人: LIPKENS, Bart , PRESZ, Walter M. Jr. , CHITALE, Kedar , KENNEDY, Thomas J. III , ROSS-JOHNSRUD, Ben
CPC分类号: C12N13/00 , A61M1/3678 , B01D21/283 , B01L3/502761 , B01L2300/0864 , B01L2300/0867 , B01L2400/0436 , B01L2400/0439 , C12M47/04 , G01N15/1484 , G01N2015/0053 , G01N2015/1006 , G01N2015/149
摘要: Devices for separating materials from a host fluid are disclosed. The devices include a flow chamber, an ultrasonic transducer, and a reflector. The ultrasonic transducer and reflector create an angled acoustic standing wave oriented at an angle relative to the direction of mean flow through the flow chamber. The angled acoustic standing wave results in an acoustic radiation force having an axial force component that deflects the materials, so that the materials and the host fluid can thus be separated. The angled acoustic standing wave can be oriented at an angle of about 20° to about 70° relative to the direction of mean flow through the flow chamber to deflect, collect, differentiate, or fractionate the materials from the fluid flowing through the device at flow rates of about 400 mL/min up to about 700 mL/min.
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公开(公告)号:EP3737738A1
公开(公告)日:2020-11-18
申请号:EP19738317.7
申请日:2019-01-09
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9.
公开(公告)号:EP3319739A1
公开(公告)日:2018-05-16
申请号:EP16753490.8
申请日:2016-07-09
发明人: LIPKENS, Bart , PRESZ, Walter, M., Jr. , CHITALE, Kedar , KENNEDY, Thomas, J., III , GILMANSHIN, Rudolf , MEALEY, Dane , DUTRA, Brian , SOKOLOWSKI, David
CPC分类号: B06B1/0238 , B01D21/0009 , B01D21/283 , B06B1/0644 , B06B3/04 , C12M47/02 , H03H9/17
摘要: An acoustophoretic device is disclosed. The acoustophoretic device includes an acoustic chamber, an ultrasonic transducer, and a reflector. The ultrasonic transducer includes a piezoelectric material driven by a voltage signal to create a multi-dimensional acoustic standing wave in the acoustic chamber emanating from a non-planar face of the piezoelectric material. A method for separating a second fluid or a particulate from a host fluid is also disclosed. The method includes flowing the mixture through an acoustophoretic device. A voltage signal is sent to drive the ultrasonic transducer to create the multi-dimensional acoustic standing wave in the acoustic chamber such that the second fluid or particulate is continuously trapped in the standing wave, and then agglomerates, aggregates, clumps, or coalesces together, and subsequently rises or settles out of the host fluid due to buoyancy or gravity forces, and exits the acoustic chamber.
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