公开(公告)号：EP2430427A1

公开(公告)日：2012-03-21

申请号：EP10730694.6

申请日：2010-05-12

申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. ,  Siemens AG

发明人： SCHREIBER, Jürgen ,  OPITZ, Jörg ,  GERICH, Carola ,  FEHRE, Jens ,  SALOMON, Georg ,  NANKE, Ralf

IPC分类号： G01N21/64 ,  A61B5/00

CPC分类号： G01N21/6486 ,  A61B5/0059 ,  G01N21/6408

摘要： The invention relates to a method and a device for detecting tumorous living cell tissue. It also relates to a method and a device for detecting tumorous cell tissue in collected samples of living cell tissue. In the method, a radiation source is used to emit electromagnetic radiation in a locally defined manner onto cell tissue and, after the radiation source has been switched off, the decay behaviour of the inherent fluorescence intensity excited in the cell tissue by the electromagnetic radiation is detected on the cell tissue by a detector, with temporal and spectral resolution and with a known scanning rate or known scanning rates for at least one wavelength. With the measured intensity values that are obtained, the difference autocorrelation function C(t) of the intensity decay behaviour is determined, from which the fractal dimension D
F for the respective irradiated cell tissue is calculated, and the value of the fractal dimension D
F is used for a classification in respect of a tumorous invasion of the respective irradiated cell tissue.

摘要翻译： 本发明涉及用于识别肿瘤活细胞组织的方法和装置。 此外，本发明还涉及用于识别收集的活细胞组织样品中的肿瘤细胞组织的方法和装置。 在该方法中，电磁辐射通过辐射源以局部定义发射到细胞组织上，并且在放射源失活之后，在细胞组织处检测由电磁辐射激发的细胞组织的固有荧光强度的衰变行为 以使用检测器的至少一个波长的已知采样速率以时间分辨和频谱分辨的方式。 使用确定的测量强度值来确定强度衰减行为的差分自相关函数C（t），并且根据此计算各个照射的细胞组织的分形维数DF，并且使用分形维数DF的值进行分类， 相对于各自照射的细胞组织中肿瘤的存在。

公开(公告)号：EP2409165A1

公开(公告)日：2012-01-25

申请号：EP09775848.6

申请日：2009-03-16

申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. ,  Technische Universität Dresden

发明人： HEUER, Henning ,  STRIEGLER, André ,  OPITZ, Jörg ,  KOPYCINSKA-MÜLLER, Malgorzata ,  NAUMANN, Sascha

IPC分类号： G01Q60/38

CPC分类号： G01Q60/38 ,  G01Q70/10

摘要： The invention relates to a scanning force microscope, which can be used for different examinations of the surfaces of samples. The aim of the invention is to provide a scanning force microscope, by which surface regions of samples can be detected, which are oriented at a steeply inclined angle with respect to the central longitudinal axis of a cantilever of the scanning force microscope. In the scanning force microscope according to the invention, at least one sensor tip is disposed on the cantilever, the sensitive region of which is disposed at a distance from the cantilever. To this end, the cantilever which is held on a face and can be prompted to oscillate using at least one actuator can be moved with the sensor tip and sample relative to each other along at least one axis. The sensitive region of a sensor tip and/or the base of a sensor tip are disposed on the cantilever with a distance and next to the center longitudinal axis of the cantilever. Either alone or additionally, the sensor tip can be angled or curved at an angle of

摘要翻译： 本发明涉及一种扫描力显微镜，其可用于样品表面的不同检查。 本发明的目的是提供一种扫描力显微镜，通过该扫描力显微镜可以检测样品的表面区域，其相对于扫描力显微镜的悬臂的中心纵向轴线以陡峭的倾斜角度取向。 在根据本发明的扫描力显微镜中，至少一个传感器尖端设置在悬臂上，其敏感区域布置在与悬臂相距一定距离处。 为此，可以使用至少一个致动器保持在面上并且可以促使其振动的悬臂随着传感器尖端和样本沿着至少一个轴线相对于彼此移动。 传感器尖端的敏感区域和/或传感器尖端的基底以悬臂的中心纵向轴线的一定距离布置在悬臂上。 无论是单独还是附加地，传感器尖端可以相对于悬臂的中心纵轴以<90°的角度成角度或弯曲。

公开(公告)号：EP3820539A1

公开(公告)日：2021-05-19

申请号：EP19730776.2

申请日：2019-06-14

申请人： FRAUNHOFER-GESELLSCHAFT zur Förderung der angewandten Forschung e.V.

发明人： BALAKIN, Sascha ,  OPITZ, Jörg

IPC分类号： A61L27/34 ,  A61L27/54 ,  A61L27/58 ,  A61L31/10 ,  A61L31/14 ,  A61L31/16

公开(公告)号：EP2646804A1

公开(公告)日：2013-10-09

申请号：EP11820888.3

申请日：2011-11-28

申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.

发明人： HAERTLING, Thomas ,  MAYER, Anton ,  OPITZ, Jörg ,  SCHREIBER, Jürgen ,  DERENKO, Susan ,  WETZEL, Christiane

IPC分类号： G01N21/64 ,  G01T1/10

CPC分类号： G01N21/643 ,  A61L2/087 ,  A61L2/28 ,  A61L2202/24 ,  C21D1/54 ,  C21D8/1272 ,  C21D11/00 ,  C21D2201/05 ,  G01N21/6408

摘要： The invention relates to a method and device (10) for testing treatments (14) which introduce energy into objects (1), wherein the method comprises the following steps: bonding at least one chemical, optically luminescent compound (3) onto at least one indicator element (6) for testing the treatments (14), wherein at least one luminescence property of the chemical compound (3) can be changed; assigning at least one indicator element (6) to the object (1), wherein the indicator element (6) and the object (1) are simultaneously subjected to the same conditions of the energy-introducing treatment (14); changing the luminescence property of the chemical compound (3), wherein the level of the change to the luminescence property depends upon the energy-introducing treatment (14; irradiating (13) the chemical compound (3) with electromagnetic radiation directed onto the indicator (6) for excitation of the luminescence during the energy-introducing treatment or following the energy-introducing treatment (14) in order to demonstrate the instantaneous energy-introducing treatment (14) or the energy-introducing treatment (14) carried out; time-resolved and/or spectrally-resolved detection of electromagnetic radiation (12) emitted as a result of luminescence of the chemical compound of the indicator element (6) during the luminescence-emitting irradiation (13) or after the luminescence-emitting irradiation (13) is switched off; providing time-resolved and/or spectrally-resolved detection signals from a detector (5) to at least one evaluation unit (9) in which: - the detection signals (17) and reference signals (18) which are stored in a memory (16) and which consist of at least one previously determined reference value and/or at least one reference wavelength or of at least one reference wavelength spectrum are compared and - the energy-introducing treatment (14) carried out on the object (1) is demonstrably established; and displaying same in a display unit (15) after testing for the presence of at least one energy input (14) in the object (1) on the basis of the achieved changed luminescence property of the chemical compound (3).