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1.发明公开METHOD FOR PRODUCTION OF CHROMATOGRAPHY MEDIA 审中-公开VERFAHREN ZUR HERSTELLUNG VON CHROMATOGRAPHIEMEDIEN
公开(公告)号：EP2563509A1
公开(公告)日：2013-03-06
申请号：EP11775370.7
申请日：2011-04-20
IPC分类号： B01J20/285 , B01D15/18 , G01N30/36
CPC分类号： B01J39/165 , B01J20/28004 , B01J20/286 , B01J20/3242 , B01J20/3293 , B01J39/17 , B01J47/018
摘要： The present invention relates to a method and means to produce chromatography media having improved pressure-flow properties. More closely, the invention relates to bimodal particle size distribution and the use of layer functionalisation as means to change pressure-flow properties of chromatography media. The invention relates to a method for production of chromatography media having improved pressure-flow properties, comprising mixing large beads/particles, comprising an inner core and an outer functionalized shell/lid, with smaller beads/particles, wherein the ratio of the particle size of large and small beads: [D50V for large particles/D50V for small particles]>1.2, and wherein the volume ratio of large and small beads in the column: [Total volume of large beds/Total volume beads] is in the range 0.05-0.9.
摘要翻译： 本发明涉及产生具有改进的压力 - 流动性质的色谱介质的方法和方法。 更具体地说，本发明涉及双峰粒度分布和层功能化作为改变色谱介质压力流动性质的手段。 本发明涉及一种用于制备具有改进的压力流动性质的色谱介质的方法，包括将具有较小珠/颗粒的包含内芯和外部功能化壳/盖的大珠/颗粒混合，其中颗粒尺寸 大颗粒和小珠：[D50V为大颗粒/ D50V为小颗粒]> 1.2，其中柱中大小珠的体积比为[大珠/总体积珠的总体积]在0.05 -0.9。
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公开(公告)号：EP2646146A1
公开(公告)日：2013-10-09
申请号：EP11845677.1
申请日：2011-11-29
IPC分类号： B01J20/285 , B01J20/32
CPC分类号： G01N1/28 , B01J20/28009 , B01J20/286 , B01J20/3208 , B01J20/3217 , B01J20/327 , B01J20/3274 , B01J20/3285 , B01J20/3293 , Y10T436/2525
摘要： The present invention relates to a buffering composition, more closely a composition comprising porous matrix-enclosed buffering agent(s) giving a stabilisation of pH when applied in for example aqueous environment. The composition comprises buffering agent(s) enclosed in a first porous matrix with an impenetrability corresponding to a fractionation range for globular proteins and peptides of
摘要翻译： 本发明涉及缓冲组合物，更接近包含多孔基质封装的缓冲剂的组合物，所述缓冲剂在例如含水环境中施用时使pH稳定。 该组合物包含包封在第一多孔基质中的缓冲剂，其具有对应于球状蛋白质和肽<10000g / mol，优选<5000g / mol，更优选<700g / mol的分级范围的不可渗透性。
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公开(公告)号：EP2268393A1
公开(公告)日：2011-01-05
申请号：EP09735164.7
申请日：2009-04-21
发明人： BERGSTRÖM, Jan , GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , NORRMAN, Nils , SÖDERMAN, Tobias, E.
IPC分类号： B01J20/285 , B01D15/34
CPC分类号： B01D15/327 , B01J20/28009 , B01J20/285 , B01J20/287 , B01J20/3212 , B01J20/3293 , B01J39/26 , C07K1/20 , C07K16/00 , C12N7/00 , C12N2760/16151 , G01N1/405
摘要： The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation mediumfor purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.
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公开(公告)号：EP2167229A1
公开(公告)日：2010-03-31
申请号：EP08794090.4
申请日：2008-07-22
IPC分类号： B01J20/289
CPC分类号： B01J20/289 , B01D15/362 , B01D15/363 , B01J20/3274 , B01J20/328
摘要： The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.
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公开(公告)号：EP2590736A1
公开(公告)日：2013-05-15
申请号：EP11803895.9
申请日：2011-06-28
IPC分类号： B01J20/285 , B01D15/36 , B01J39/26 , B01J41/20 , C07K1/18
CPC分类号： B01D15/361 , C07K1/18
摘要： The present invention relates to a method for running ion exchange chromatography on a media comprising shell beads having an inner porous core and an outer shell, wherein the inner core is provided with ligands whose charge changes with pH and the shell is provided with charged ion exchange ligands, the method comprising the following steps: a) adsorbing sample molecules on the shell ligands at a first pH; b) causing a discharge of the inner core ligands at a second pH by addition of a buffer substance that is able to increase its charge having the same sign/type as that of the core ligands, which at the same time causes release of ions from the inner core ligands and thereby an increase in ionic strength that displaces the sample molecules from the shell ligands i.e. causes an elution.
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公开(公告)号：EP2536499A1
公开(公告)日：2012-12-26
申请号：EP11744970.2
申请日：2011-02-16
IPC分类号： B01J20/285 , B01D15/08 , B01J20/286
CPC分类号： B01J20/3293 , B01D15/361 , B01D15/3804 , B01J20/28004 , B01J20/286 , B01J20/3206 , B01J20/3248 , B01J20/3285 , B01J39/26 , B01J41/20 , C07K1/18
摘要： The present invention relates to a method of producing novel chromatography media and use thereof for purification of biomolecules, such as proteins. The chromatography media comprises shell beads having an inner porous core and an outer shell. The method comprises providing buffering ligands in the core of the beads, and providing binding ligands aimed for biomolecule binding in the outer shell of the beads. This method makes it possible to optimize binding properties and buffering properties independently of each other which is especially advantageous for production of chroma to focusing media.
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公开(公告)号：EP0966488B1
公开(公告)日：2006-10-11
申请号：EP98908406.6
申请日：1998-03-04
CPC分类号： C08B37/0039 , B01J20/3212 , B01J20/3234 , C08B37/0021
摘要： Process for, in layers of a porous matrix, exhibiting the groups A, introducing a functionality using a reagent I, introducing the functionality by a reaction with the groups A. The characterizing feature is that the matrix is contacted with a functional deficiency of reagent I and that conditions and reagent I are chosen so that the reaction between reagent I and the groups A is more rapid than diffusion of reagent I in the matrix.
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公开(公告)号：EP1677887A1
公开(公告)日：2006-07-12
申请号：EP04800246.3
申请日：2004-11-01
CPC分类号： B01J20/3285 , B01D15/364 , B01J20/286 , B01J20/3244
摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.
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