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公开(公告)号：EP1235749B1
公开(公告)日：2011-01-19
申请号：EP00988740.7
申请日：2000-11-22
CPC分类号： B01J41/20 , B01D15/327 , B01J41/09 , C02F1/42
摘要： A method for the removal of a substance, which has a negative charge and which and which is present in an aqueous liquid (I). The method comprises the steps of: (i) contacting the liquid with an anion-exchanger (1) that comprises mixed mode anion-exchanging ligands in which there is a positively charged nitrogen allowing binding of the substance to the anion-exchanger; and (ii) desorbing said substance from said anion-exchanger. The characteristic feature is that (A) the mixed mode ligands have a thioether linkage within a distance of 1-7 atoms from their positively charged atom, and (B) the anion-exchanger (1) (i) is capable of binding the substance of interest in an aqueous reference liquid (II) at an ionic strength corresponding to 0.25 M NaCl, and (ii) permits in the pH interval 2-12 a maximal breakthrough capacity for the substance which is ≥200 % of the breakthrough capacity of the substance for Q-Sepharose Fast Flow (anion-exchanger 2).
摘要翻译： 一种去除具有负电荷且存在于含水液体（I）中的物质的方法。 该方法包括以下步骤：（i）使液体与阴离子交换剂（1）接触，阴离子交换剂（1）包含混合型阴离子交换配体，其中存在允许物质与阴离子交换剂结合的带正电的氮; 和（ii）从所述阴离子交换剂解吸所述物质。 其特征是（A）混合模式的配体在距离其正电荷原子1-7个原子的距离内具有硫醚键，和（B）阴离子交换剂（1）（i）能够结合物质 在对应于0.25M NaCl的离子强度下在水性参考液体（II）中感兴趣，和（ii）允许在pH 2-12的范围内物质的最大穿透能力≥该穿透能力的200％ 用于Q-Sepharose Fast Flow（阴离子交换剂2）的物质。
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公开(公告)号：EP2167229A1
公开(公告)日：2010-03-31
申请号：EP08794090.4
申请日：2008-07-22
IPC分类号： B01J20/289
CPC分类号： B01J20/289 , B01D15/362 , B01D15/363 , B01J20/3274 , B01J20/328
摘要： The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.
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公开(公告)号：EP2748181A1
公开(公告)日：2014-07-02
申请号：EP12837180.4
申请日：2012-09-28
IPC分类号： C07K1/18 , A61K38/28 , B01D15/08 , B01D15/26 , B01D15/36 , B01J20/285 , B01J20/286 , B01J20/32 , C07K14/06 , C07K14/62
CPC分类号： C07K14/62 , B01D15/361 , B01D15/362 , B01J20/286 , B01J20/288 , B01J20/321 , B01J20/3212 , B01J20/3293 , B01J39/26
摘要： The present invention is within the field of biomolecule purification. More closely the invention relates to chromatographic purification of insulin using a specific kind of shell beads having an inner core and an outer functionalized layer. The method enables purification at high flow rates and high purity, over 90%.
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公开(公告)号：EP1697416B1
公开(公告)日：2008-04-09
申请号：EP04809180.5
申请日：2004-12-21
IPC分类号： C07K16/06
CPC分类号： B01J20/262 , B01J20/267 , B01J20/28019 , B01J20/28023 , B01J20/28033 , B01J20/28042 , B01J20/286 , B01J20/289 , B01J20/3092 , B01J20/3208 , B01J20/3212 , B01J20/3219 , B01J20/3246 , B01J20/3248 , B01J20/3251 , B01J20/3255 , B01J20/3272 , B01J20/3285 , B01J2220/54 , B01J2220/58 , C07K16/065 , Y10T428/249963
摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilised, wherein said ligands comprise at least one aliphatic sulphonamide. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to a separation matrix that comprises aliphatic sulphonamide ligands.
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公开(公告)号：EP1718386A1
公开(公告)日：2006-11-08
申请号：EP05722175.6
申请日：2005-02-25
发明人： GRÖNBERG, Anna , JOHANSSON, Bo-Lennart , JOHANSSON, Hans J. , MALOISEL, Jean-Luc , THEVENIN, Nicolas
CPC分类号： B01J20/3253 , B01D15/3847 , B01J20/3242 , B01J20/3246 , B01J20/3255 , B01J20/3285
摘要： The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group con-sisting of C, S and O, and the cation exchanging group is a weak cation exchanger.
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6.发明授权
公开(公告)号：EP1345694B1
公开(公告)日：2006-08-02
申请号：EP01985906.5
申请日：2001-12-19
摘要： Method for desalting an aqueous liquid containing a charged substance comprising the steps of:(i) contacting liquid (I) with an ion-exchanger (1) under conditions permitting binding between ion-exchanger and substance, said ion-exchanger comprising a base matrix carrying an ion-exchange ligand (Ligand 1) with the opposite charge compared to the substance, (ii) desorbing said substance from said ion-exchanger by the use of a liquid (liquid (II)). Wherein: (A) ion-exchanger (1) is an ion-exchanger::(a) that can bind the substance in an aqueous reference liquid at an ionic strength corresponding to 0.1 M NaCl, preferably 0.25 M NaCl; and (b) permits a breakthrough capacity at the pH provided by liquid (I) which is more than 2 mg/ml of gel, at a breakthrough of 10% and linear flow velocity of 300 cm/h;and(B) in step (ii) the pH of liquid (II) is adjusted to a pH value where the charge difference between the substance, the ligand and/or the ion-exchanger is lowered.
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公开(公告)号：EP2646146A1
公开(公告)日：2013-10-09
申请号：EP11845677.1
申请日：2011-11-29
IPC分类号： B01J20/285 , B01J20/32
CPC分类号： G01N1/28 , B01J20/28009 , B01J20/286 , B01J20/3208 , B01J20/3217 , B01J20/327 , B01J20/3274 , B01J20/3285 , B01J20/3293 , Y10T436/2525
摘要： The present invention relates to a buffering composition, more closely a composition comprising porous matrix-enclosed buffering agent(s) giving a stabilisation of pH when applied in for example aqueous environment. The composition comprises buffering agent(s) enclosed in a first porous matrix with an impenetrability corresponding to a fractionation range for globular proteins and peptides of
摘要翻译： 本发明涉及缓冲组合物，更接近包含多孔基质封装的缓冲剂的组合物，所述缓冲剂在例如含水环境中施用时使pH稳定。 该组合物包含包封在第一多孔基质中的缓冲剂，其具有对应于球状蛋白质和肽<10000g / mol，优选<5000g / mol，更优选<700g / mol的分级范围的不可渗透性。
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公开(公告)号：EP2268393A1
公开(公告)日：2011-01-05
申请号：EP09735164.7
申请日：2009-04-21
发明人： BERGSTRÖM, Jan , GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , NORRMAN, Nils , SÖDERMAN, Tobias, E.
IPC分类号： B01J20/285 , B01D15/34
CPC分类号： B01D15/327 , B01J20/28009 , B01J20/285 , B01J20/287 , B01J20/3212 , B01J20/3293 , B01J39/26 , C07K1/20 , C07K16/00 , C12N7/00 , C12N2760/16151 , G01N1/405
摘要： The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation mediumfor purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.
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公开(公告)号：EP2129461A1
公开(公告)日：2009-12-09
申请号：EP08724138.6
申请日：2008-03-20
IPC分类号： B01J20/289 , B01D15/32
CPC分类号： B01J20/289 , B01D15/32 , B01D15/327 , B01D15/361 , B01D15/3804 , B01D15/3847 , B01J20/262 , B01J20/267 , B01J20/286 , B01J20/3212 , B01J20/3219 , B01J20/3251 , B01J20/3272 , B01J20/328 , B01J20/3285 , B01J2220/54 , B01J2220/58 , C07K1/20 , C07K16/00
摘要： The present invention relates to a separation matrix comprised of a porous or non-porous support to which ligands have been immobilised, wherein said ligands comprise at least one aliphatic sulfamide. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulins, such as IgG, Fab fragments, fusion proteins containing immunoglobulins etc, by adsorption to a separation matrix that comprises the aliphatic sulfamide ligands of the invention.
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公开(公告)号：EP2590736A1
公开(公告)日：2013-05-15
申请号：EP11803895.9
申请日：2011-06-28
IPC分类号： B01J20/285 , B01D15/36 , B01J39/26 , B01J41/20 , C07K1/18
CPC分类号： B01D15/361 , C07K1/18
摘要： The present invention relates to a method for running ion exchange chromatography on a media comprising shell beads having an inner porous core and an outer shell, wherein the inner core is provided with ligands whose charge changes with pH and the shell is provided with charged ion exchange ligands, the method comprising the following steps: a) adsorbing sample molecules on the shell ligands at a first pH; b) causing a discharge of the inner core ligands at a second pH by addition of a buffer substance that is able to increase its charge having the same sign/type as that of the core ligands, which at the same time causes release of ions from the inner core ligands and thereby an increase in ionic strength that displaces the sample molecules from the shell ligands i.e. causes an elution.
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