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    AFFINITY PURIFICATION METHODS INVOLVING AMINO ACID MIMETICS AS ELUTION REAGENTS.
    1.
    发明公开
    AFFINITY PURIFICATION METHODS INVOLVING AMINO ACID MIMETICS AS ELUTION REAGENTS. 失效
    AMINOSÄURE-MIMETIKA ALS ELUTIONSREAGENTIEN INAFFINITÄTSREINIGUNGSMETHODEN。

    公开(公告)号：EP0663925A4

    公开(公告)日：1997-11-19

    申请号：EP93923139

    申请日：1993-09-27

    申请人： GEN HOSPITAL CORP

    发明人： SEED BRIAN

    IPC分类号： C07K1/22 ,  C07K14/31 ,  C07K16/06 ,  C07K16/14 ,  C07K19/00 ,  C07K1/00 ,  C07K15/28 ,  C07K17/14

    CPC分类号： C07K1/22 ,  C07K16/065

    摘要： Disclosed is a method of isolating a protein from a sample, involving (i) providing a first molecule which is capable of forming an affinity complex with the protein; (ii) contacting the sample with the first molecule under conditions which allow affinity complex formation; (iii) isolating the complex; (iv) treating the complex with a second molecule, the second molecule mimicking an amino acid residue of either the protein or the first molecule which is critical to the complex formation, so that the second molecule disrupts the complex; causing the release of the protein from the complex; and (v) isolating the protein. According to one embodiment, the amino acid mimetic imidazole is used as a very gentle elution reagent to disrupt a protein A-antibody fusion protein complex, a technique which has general application for the isolation of antibodies or recombinant antibody fusion proteins.

    摘要翻译： 公开了一种从样品中分离蛋白质的方法，其包括（i）提供能够与蛋白质形成亲和复合物的第一分子; （ii）在允许形成亲和性复合物的条件下使样品与第一分子接触; （iii）分离复合物; （iv）用第二分子处理所述复合物，所述第二分子模拟所述蛋白质或所述第一分子的氨基酸残基，所述氨基酸残基对所述复合体形成是关键的，以致所述第二分子破坏所述复合物; 引起蛋白质从复合物的释放; 和（v）分离蛋白质。 根据一个实施方案，氨基酸模拟咪唑用作非常温和的洗脱剂以破坏蛋白A抗体融合蛋白复合物，该技术具有用于分离抗体或重组抗体融合蛋白的一般应用。