公开(公告)号：EP1679376A3

公开(公告)日：2006-08-16

申请号：EP06003302.4

申请日：1995-08-07

申请人： GENVEC, INC.

发明人： Wickham, Thomas J. ,  Kovesdi, Imre ,  Brough, Douglas E. ,  McVey, Duncan L. ,  Bruder, Joseph T.

IPC分类号： C12N15/86 ,  C12N15/87 ,  C12N7/01 ,  C12N15/34 ,  A61K48/00

CPC分类号： C12N15/87 ,  A61K48/00 ,  C07K14/005 ,  C07K14/70546 ,  C07K2319/00 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2710/14043 ,  C12N2710/14143 ,  C12N2810/40 ,  C12N2810/405 ,  C12N2810/60 ,  C12N2810/6018 ,  C12N2810/80 ,  C12N2810/855 ,  C12N2810/856 ,  C12N2810/859

摘要： A recombinant adenovirus comprising a chimeric penton base protein, wherein an RGD amino acid sequence is deleted from the penton base protein, a method of gene therapy involving the use of such adenovirus, and adenoviral transfer vectors for the generation of such recombinant adenovirus are provided.

公开(公告)号：EP1518933A1

公开(公告)日：2005-03-30

申请号：EP04028530.6

申请日：1996-11-27

申请人： GENVEC, INC.

发明人： Wickham, Thomas J. ,  Kovesdi, Imre ,  Brough, Douglas E.

IPC分类号： C12N15/86 ,  C07K14/075 ,  C12N7/01 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C07K2319/00 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2810/40 ,  C12N2810/405 ,  C12N2810/60

摘要： The present invention provides a chimeric adenovirus coat protein, which differs from the wild-type coat protein by the introduction of a nonnative amino acid sequence. Such a chimeric adenovirus coat protein according to the invention is able to direct entry into cells of a vector comprising the coat protein that is more efficient than entry into cells of a vector that is identical except for comprising a wild-type adenovirus coat protein rather than the chimeric adenovirus coat protein. The chimeric coat protein preferably is a fiber, hexon, or penton protein, The present invention also provides an adenoviral vector that comprises the chimeric adenovirus coat protein, as well as methods of constructing and using such a vector.

摘要翻译： 本发明提供了通过引入非氨基酸序列与野生型外壳蛋白不同的嵌合腺病毒外壳蛋白。 根据本发明的这种嵌合腺病毒外壳蛋白能够直接进入包含外壳蛋白的载体的细胞中，所述载体比除了包含野生型腺病毒外壳蛋白的载体以外的载体的细胞进入更有效，而不是 嵌合腺病毒外壳蛋白。 嵌合外壳蛋白优选为纤维，六邻体或五聚体蛋白质。本发明还提供了包含嵌合腺病毒外壳蛋白的腺病毒载体，以及构建和使用这种载体的方法。

公开(公告)号：EP1679376A2

公开(公告)日：2006-07-12

申请号：EP06003302.4

申请日：1995-08-07

申请人： GENVEC, INC.

发明人： Wickham, Thomas J. ,  Kovesdi, Imre ,  Brough, Douglas E. ,  McVey, Duncan L. ,  Bruder, Joseph T.

IPC分类号： C12N15/86 ,  C12N15/87 ,  C12N7/01 ,  C12N15/34 ,  A61K48/00

CPC分类号： C12N15/87 ,  A61K48/00 ,  C07K14/005 ,  C07K14/70546 ,  C07K2319/00 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2710/14043 ,  C12N2710/14143 ,  C12N2810/40 ,  C12N2810/405 ,  C12N2810/60 ,  C12N2810/6018 ,  C12N2810/80 ,  C12N2810/855 ,  C12N2810/856 ,  C12N2810/859

摘要： A recombinant adenovirus comprising a chimeric penton base protein, wherein an RGD amino acid sequence is deleted from the penton base protein, a method of gene therapy involving the use of such adenovirus, and adenoviral transfer vectors for the generation of such recombinant adenovirus are provided.

摘要翻译： 提供了一种重组腺病毒，其包含嵌合五羟色胺基蛋白，其中从五羟基蛋白缺失RGD氨基酸序列，涉及使用这种腺病毒的基因治疗方法和用于产生这种重组腺病毒的腺病毒转移载体。