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1.发明公开VECTORS, CELLS AND PROCESSES FOR PYRIMIDINE DEOXYRIBONUCLEOSIDES PRODUCTION 有权载体，细胞及其制造方法PYRIMIDINDEOXYRIBONUCLEOSIDEN
公开(公告)号：EP1190064A1
公开(公告)日：2002-03-27
申请号：EP00940557.2
申请日：2000-06-30
申请人： GLAXO GROUP LIMITED
IPC分类号： C12N15/52 , C12N15/53 , C12N15/54 , C12N15/55 , C12N9/02 , C12N9/10 , C12N9/12 , C12N9/78 , C12N1/19 , C12N1/21 , C12R1/19 , C12P19/38
CPC分类号： C12N9/0036 , C12N9/0093 , C12N9/1007 , C12N9/1205 , C12N9/78 , C12N15/52 , C12P19/385
摘要： Novel DNA constructs and host cells comprising the same are disclosed. DNA constructs comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin or a uridine kinase gene and/or a dCTP deaminase gene. In preferred embodiments the constructs comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin further comprise DNA sequences encoding for thymidylate synthase and/or transcription units comprising sequences encoding for uridine kinase preferably together with dCTP deaminase. In particularly preferred embodiments, the host cells comprise constructs having all of the above characteristics wherein the host cell displays repressed or no uracil DNA glycosylase activity. This may be achieved by removal of the host cell ung gene. Use of host cells in the manufacture of pyrimidine deoxyribonucleotides e.g. thymidine is also disclosed.
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公开(公告)号：EP1981972B1
公开(公告)日：2013-03-20
申请号：EP07704356.0
申请日：2007-02-05
申请人： GLAXO GROUP LIMITED
发明人： ANDERSON, David, Martin , COLLIS, Andrew, John , LIU, Lin , PODKOVYROV, Sergey , PRESTON, Christopher
CPC分类号： C12N15/52 , C12P19/38 , C12P19/385
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公开(公告)号：EP1981972A1
公开(公告)日：2008-10-22
申请号：EP07704356.0
申请日：2007-02-05
申请人： GLAXO GROUP LIMITED
发明人： ANDERSON, David, Martin , COLLIS, Andrew, John , LIU, Lin , PODKOVYROV, Sergey , PRESTON, Christopher
CPC分类号： C12N15/52 , C12P19/38 , C12P19/385
摘要： Novel organisms, including DNA construct host cell combinations, are disclosed. The organisms comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for enzymes which promote the supply of single carbon units for the conversion of dUMP to dTMP. Examples include: dihydrofolate reductase genes e.g. T4 frd; Serine Hydroxymethyltransferase genes e.g. glyA; 3-phosphoglycerate dehydrogenase genes e.g. serA; and THF synthase genes e.g. ADE3. The organisms are used in a biological method of producing thymidine with significantly reduced levels of uridine.
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4.发明授权VECTORS, CELLS AND PROCESSES FOR PYRIMIDINE DEOXYRIBONUCLEOSIDES PRODUCTION 有权载体，细胞及其制造方法PYRIMIDINDEOXYRIBONUCLEOSIDEN
公开(公告)号：EP1190064B1
公开(公告)日：2007-08-01
申请号：EP00940557.2
申请日：2000-06-30
申请人： GLAXO GROUP LIMITED
IPC分类号： C12N15/52 , C12N15/53 , C12N15/54 , C12N15/55 , C12N9/02 , C12N9/10 , C12N9/12 , C12N9/78 , C12N1/19 , C12N1/21 , C12R1/19 , C12P19/38
CPC分类号： C12N9/0036 , C12N9/0093 , C12N9/1007 , C12N9/1205 , C12N9/78 , C12N15/52 , C12P19/385
摘要： Novel DNA constructs and host cells comprising the same are disclosed. DNA constructs comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin or a uridine kinase gene and/or a dCTP deaminase gene. In preferred embodiments the constructs comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin further comprise DNA sequences encoding for thymidylate synthase and/or transcription units comprising sequences encoding for uridine kinase preferably together with dCTP deaminase. In particularly preferred embodiments, the host cells comprise constructs having all of the above characteristics wherein the host cell displays repressed or no uracil DNA glycosylase activity. This may be achieved by removal of the host cell ung gene. Use of host cells in the manufacture of pyrimidine deoxyribonucleotides e.g. thymidine is also disclosed.
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