公开(公告)号：EP1572912A2

公开(公告)日：2005-09-14

申请号：EP03716849.9

申请日：2003-03-27

申请人： Gala Design, Inc.

发明人： BREMEL, Robert, D. ,  YORK, Dona ,  BLECK, Gregory, T. ,  EAKLE, Kurt, A.

IPC分类号： C12N1/00

CPC分类号： C12N15/86 ,  C12N2510/02 ,  C12N2740/13043 ,  C12N2740/15043 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/48 ,  C12N2830/85 ,  C12N2840/203 ,  C12P21/02

摘要： The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector comprising an exogenous gene. The present invention further relates to the use of integrating vectors lacking a selectable marker and growth of host cells containing such vectors in the absence of selection. The present invention further provides methods of expressing increased levels of protein in host cells using such vectors.

公开(公告)号：EP1751282A2

公开(公告)日：2007-02-14

申请号：EP05705678.0

申请日：2005-01-14

申请人： Gala Design, Inc.

发明人： BLECK, Gregory, T. ,  BREMEL, Robert, D. ,  MILLER, Linda, U.

IPC分类号： C12N15/09

CPC分类号： C12P21/02 ,  C07K14/005 ,  C07K14/76 ,  C07K16/1228 ,  C07K16/1282 ,  C07K16/30 ,  C07K16/3007 ,  C07K16/3046 ,  C07K2319/00 ,  C07K2319/02 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8216 ,  C12N15/8241 ,  C12N15/85 ,  C12N15/86 ,  C12N2510/02 ,  C12N2740/13022 ,  C12N2740/13023 ,  C12N2740/13043 ,  C12N2740/13051 ,  C12N2760/16122 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/48 ,  C12N2830/85 ,  C12N2840/203 ,  C12N2840/85

摘要： The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector comprising an exogenous gene and methods of making such host cells by serial transduction or transfection. The present invention further provides methods of expressing increased levels of protein in host cells using such vectors.

公开(公告)号：EP1299556A2

公开(公告)日：2003-04-09

申请号：EP01950645.0

申请日：2001-06-29

申请人： Gala Design, Inc.

发明人： BREMEL, Robert, D. ,  MILLER, Linda, U. ,  BLECK, Gregory, T. ,  YORK, Donna

IPC分类号： C12N15/85 ,  C12N15/86 ,  C12N5/10 ,  G01N33/50

CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K16/1228 ,  C07K16/1282 ,  C07K16/30 ,  C07K16/3007 ,  C07K16/3046 ,  C07K2319/00 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8216 ,  C12N15/8241 ,  C12N15/85 ,  C12N2730/10122 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2740/13062 ,  C12N2750/14143 ,  C12N2810/6081 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/48 ,  C12N2830/85 ,  C12N2840/203

摘要： The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.