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1.发明公开CAPTURE PROBES IMMOBILIZABLE VIA L-NUCLEOTIDE TAIL 有权ÜBERL-NUKLEOTIDENDEN IMMOBILISIERBAREFÄNGERSONDEN
公开(公告)号：EP2616555A1
公开(公告)日：2013-07-24
申请号：EP11764910.3
申请日：2011-09-16
发明人： POLLNER, Reinhold , MAJLESSI, Mehrdad , YAMAGATA, Susan , BECKER, Michael, M. , REYNOLDS, Mark , ARNOLD, Lyle
IPC分类号： C12Q1/68
CPC分类号： C12Q1/707 , C12Q1/6837 , C12Q1/703 , Y02A50/53 , Y02A50/54 , C12Q2525/161
摘要： There is disclosed a method of capturing a target nucleic acid, comprising: contacting a target nucleic acid with a capture probe and an immobilized probe, the capture probe comprising a first segment that binds to the target nucleic acid and a second segment that binds to the immobilized probe, wherein the second segment of the capture probe and the immobilized probe comprise L-nucleic acids that can hybridize to one another, wherein the target nucleic acid binds to the first segment of the capture probe, and the second segment of the capture probe binds to the target, thereby capturing the target nucleic acid.
摘要翻译： 本发明提供了通过可以在固定化探针中结合互补L-核酸的L-核酸尾端固定的嵌合捕获探针。 捕获探针可用于从样品捕获靶核酸。 捕获探针尾部的L-核酸与固定化探针中的互补L-核酸结合，具有与否则相当于D-核酸的亲和力。 然而，捕获探针尾和固定化探针的L-核酸不与存在于含有靶核酸的样品中的D-核酸形成稳定的双链体。 将样品中的核酸直接与捕获探针的固定化探针或尾部的结合降低或消除，增加了测定的灵敏度和/或特异性。
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公开(公告)号：EP3511426A1
公开(公告)日：2019-07-17
申请号：EP19155762.8
申请日：2013-02-01
IPC分类号： C12Q1/6816 , C12Q1/6834
摘要： There is disclosed a target capture oligomer comprising first and second stem segments differing in length by at least two nucleobases flanking a target-binding segment complementary to a target nucleic acid, wherein under hybridizing conditions: in the absence of the target nucleic acid the target capture oligomer forms a stem-loop, intramolecular hybridization of the first and second stem segments forming the stem, and the target-binding segment forming the loop; and in the presence of the target nucleic acid, the target-binding segment hybridizes to the target nucleic acid disrupting the intramolecular hybridization of the first and second stem segments resulting in the first stem segment being accessible to hybridize to a complementary immobilized probe.
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公开(公告)号：EP2616555B1
公开(公告)日：2017-11-08
申请号：EP11764910.3
申请日：2011-09-16
发明人： POLLNER, Reinhold , MAJLESSI, Mehrdad , YAMAGATA, Susan , BECKER, Michael, M. , REYNOLDS, Mark , ARNOLD, Lyle
IPC分类号： C12Q1/68
CPC分类号： C12Q1/707 , C12Q1/6837 , C12Q1/703 , Y02A50/53 , Y02A50/54 , C12Q2525/161
摘要： There is disclosed a method of capturing a target nucleic acid, comprising: contacting a target nucleic acid with a capture probe and an immobilized probe, the capture probe comprising a first segment that binds to the target nucleic acid and a second segment that binds to the immobilized probe, wherein the second segment of the capture probe and the immobilized probe comprise L-nucleic acids that can hybridize to one another, wherein the target nucleic acid binds to the first segment of the capture probe, and the second segment of the capture probe binds to the target, thereby capturing the target nucleic acid.
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公开(公告)号：EP2809807A1
公开(公告)日：2014-12-10
申请号：EP13705870.7
申请日：2013-02-01
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/6816 , C12Q2525/301 , C12Q2527/107 , C12Q2565/501
摘要： The invention provides an improved stem-loop target capture oligomer and methods of use. Such a target capture oligomer has a target-binding segment forming a loop flanked by stem segments forming a stem. The stem segments are of unequal length. Such probes show little or no binding to immobilized probes in the absence of a target nucleic acid but offer good target sensitivity. The probes are particularly useful in multiplex methods of detection in which multiple target capture oligomers are present for detecting of multiple target nucleic acids (for example, detecting multiple polymorphic forms of a target gene).
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6.发明授权
公开(公告)号：EP2635703B1
公开(公告)日：2018-03-21
申请号：EP11782721.2
申请日：2011-11-01
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12P19/34 , C12Q1/6834 , C12Q1/686 , C12Q1/6874 , C12Q1/70 , C12Q2531/113 , C12Q2565/537
摘要： The invention provides efficient methods of preparing a target nucleic acid in a form suitable for sequencing. The methods are particularly amenable for preparing high quality nucleic acids for massively parallel sequencing. The methods involve capturing a target nucleic acid from a sample and PCR amplification of the target nucleic acid. The target nucleic acid is captured by binding to a capture probe, which in turn binds to an immobilized probe. The immobilized probe is typically immobilized via a magnetic bead. The captured target nucleic acid is PCR amplified by thermocycling without prior dissociation of the target nucleic acid from the beads. The efficiency of the method lies in part in that both the capture and amplification steps are performed in a single vessel. The amplified nucleic acid can then be sequenced.
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7.发明公开
公开(公告)号：EP2635703A1
公开(公告)日：2013-09-11
申请号：EP11782721.2
申请日：2011-11-01
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12P19/34 , C12Q1/6834 , C12Q1/686 , C12Q1/6874 , C12Q1/70 , C12Q2531/113 , C12Q2565/537
摘要： The invention provides efficient methods of preparing a target nucleic acid in a form suitable for sequencing. The methods are particularly amenable for preparing high quality nucleic acids for massively parallel sequencing. The methods involve capturing a target nucleic acid from a sample and PCR amplification of the target nucleic acid. The target nucleic acid is captured by binding to a capture probe, which in turn binds to an immobilized probe. The immobilized probe is typically immobilized via a magnetic bead. The captured target nucleic acid is PCR amplified by thermocycling without prior dissociation of the target nucleic acid from the beads. The efficiency of the method lies in part in that both the capture and amplification steps are performed in a single vessel. The amplified nucleic acid can then be sequenced.
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