公开(公告)号：EP2616555B1

公开(公告)日：2017-11-08

申请号：EP11764910.3

申请日：2011-09-16

申请人： Gen-Probe Incorporated

发明人： POLLNER, Reinhold ,  MAJLESSI, Mehrdad ,  YAMAGATA, Susan ,  BECKER, Michael, M. ,  REYNOLDS, Mark ,  ARNOLD, Lyle

IPC分类号： C12Q1/68

CPC分类号： C12Q1/707 ,  C12Q1/6837 ,  C12Q1/703 ,  Y02A50/53 ,  Y02A50/54 ,  C12Q2525/161

摘要： There is disclosed a method of capturing a target nucleic acid, comprising: contacting a target nucleic acid with a capture probe and an immobilized probe, the capture probe comprising a first segment that binds to the target nucleic acid and a second segment that binds to the immobilized probe, wherein the second segment of the capture probe and the immobilized probe comprise L-nucleic acids that can hybridize to one another, wherein the target nucleic acid binds to the first segment of the capture probe, and the second segment of the capture probe binds to the target, thereby capturing the target nucleic acid.

公开(公告)号：EP2616555A1

公开(公告)日：2013-07-24

申请号：EP11764910.3

申请日：2011-09-16

申请人： Gen-Probe Incorporated

发明人： POLLNER, Reinhold ,  MAJLESSI, Mehrdad ,  YAMAGATA, Susan ,  BECKER, Michael, M. ,  REYNOLDS, Mark ,  ARNOLD, Lyle

IPC分类号： C12Q1/68

CPC分类号： C12Q1/707 ,  C12Q1/6837 ,  C12Q1/703 ,  Y02A50/53 ,  Y02A50/54 ,  C12Q2525/161

摘要： There is disclosed a method of capturing a target nucleic acid, comprising: contacting a target nucleic acid with a capture probe and an immobilized probe, the capture probe comprising a first segment that binds to the target nucleic acid and a second segment that binds to the immobilized probe, wherein the second segment of the capture probe and the immobilized probe comprise L-nucleic acids that can hybridize to one another, wherein the target nucleic acid binds to the first segment of the capture probe, and the second segment of the capture probe binds to the target, thereby capturing the target nucleic acid.

摘要翻译： 本发明提供了通过可以在固定化探针中结合互补L-核酸的L-核酸尾端固定的嵌合捕获探针。 捕获探针可用于从样品捕获靶核酸。 捕获探针尾部的L-核酸与固定化探针中的互补L-核酸结合，具有与否则相当于D-核酸的亲和力。 然而，捕获探针尾和固定化探针的L-核酸不与存在于含有靶核酸的样品中的D-核酸形成稳定的双链体。 将样品中的核酸直接与捕获探针的固定化探针或尾部的结合降低或消除，增加了测定的灵敏度和/或特异性。

公开(公告)号：EP3327140A1

公开(公告)日：2018-05-30

申请号：EP17199730.7

申请日：2011-09-16

申请人： Gen-Probe Incorporated

发明人： POLLNER, Reinhold B. ,  MAJLESSI, Mehrdad ,  YAMAGATA, Susan ,  BECKER, Michael M ,  REYNOLDS, Mark ,  ARNOLD, Lyle J Jr

IPC分类号： C12Q1/68

CPC分类号： C12Q1/707 ,  C12Q1/6837 ,  C12Q1/703 ,  Y02A50/53 ,  Y02A50/54 ,  C12Q2525/161

摘要： There is disclosed a method of capturing a target nucleic acid, comprising: contacting a target nucleic acid with a capture probe and an immobilized probe, the capture probe comprising a first segment that binds to the target nucleic acid and a second segment that binds to the immobilized probe, wherein the second segment of the capture probe and the immobilized probe comprise L-nucleic acids that can hybridize to one another, wherein the target nucleic acid binds to the first segment of the capture probe, and the second segment of the capture probe binds to the target, thereby capturing the target nucleic acid.