公开(公告)号：EP2365071B1

公开(公告)日：2016-04-06

申请号：EP09829144.6

申请日：2009-11-26

申请人： Hokko Chemical Industry Co., Ltd.

发明人： TERAKAWA, Teruhiko ,  HASEGAWA, Hisakazu ,  ISHIMOTO, Masao ,  UTSUMI, Shigeru ,  SHOJI, Mikio ,  KAWARABAYASHI, Takeshi

IPC分类号： C12N15/00 ,  A01H5/00 ,  A61K39/00 ,  A61P25/28 ,  C12N5/10 ,  C12N15/09

CPC分类号： C12N15/8258 ,  A61K39/0005 ,  A61K2039/517 ,  C07K16/18 ,  C07K2317/13 ,  C07K2317/34

公开(公告)号：EP1026249B1

公开(公告)日：2008-12-10

申请号：EP98940641.8

申请日：1998-08-31

申请人： HOKKO CHEMICAL INDUSTRY CO. LTD. ,  Incorporated Administrative Agency National Agriculture and Bio-oriented Research Organization

发明人： WAKASA, Kyo ,  TOZAWA, Yuzuru ,  TERAKAWA, Teruhiko ,  HASEGAWA, Hisakazu

IPC分类号： C12N15/60 ,  A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N1/21 ,  C12N15/82 ,  C12N9/88

CPC分类号： C12N15/8254 ,  C12N9/88 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/8216 ,  C12N15/8251 ,  C12Y401/03027

摘要： A DNA sequence capable of encoding an enzyme protein which is the α-subunit of the first isozyme of rice anthranilate synthase (ASA); and a DNA capable of encoding an enzyme protein which is the α-subunit of the second isozyme of ASA. A transformant plant with a high tryptophan content can be regenerated by culturing vegetable cells into which a recombinant vector constructed by integrating a DNA fragment carrying the above DNA sequence into the downstream of a promoter has been transferred. The invention also provides a DNA having a promoter activity on the rice ASA gene, which is usable as a promoter for transferring a useful foreign gene into rice or other plants and then expressing the same.

公开(公告)号：EP1072685A1

公开(公告)日：2001-01-31

申请号：EP98914084.3

申请日：1998-04-17

申请人： HOKKO CHEMICAL INDUSTRY CO. LTD.

发明人： TERAKAWA, Teruhiko ,  HASEGAWA, Hisakazu ,  YAMAGUCHI, Masanori

IPC分类号： C12N15/60 ,  A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N1/21 ,  C12N15/63

CPC分类号： C12N9/88 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/8254

摘要： In accordance with this invention, there is provided a DNA sequence which can encode an enzyme protein functioning as the rice dihydrodipicolinate synthase (DHDPS). One example of the DNA sequence according to this invention is the DNA having the nucleotide sequence shown in SEQ ID No. 1 of Sequence Listing. Additionally, this invention provides the DNA having the nucleotide sequence of SEQ ID No. 5, as well as the DNA having the nucleotide sequence of SEQ ID No. 7 of Sequence Listing, as the DNA which is capable of encoding the protein having the DHDPS activity. When cultivation is made of the plant cells in which there has been introduced a recombinant vector having carried therein a DNA fragment containing the DNA of this invention as inserted at downstream of the promoter of said recombinant vector, it is feasible to regenerate from said plant cells a transgenic plant having a high lysine content.

摘要翻译： 根据本发明，提供了可以编码作为水稻二氢吡啶二羧酸合酶（DHDPS）的酶蛋白质的DNA序列。 根据本发明的DNA序列的一个实例是具有序列表的SEQ ID No.1所示的核苷酸序列的DNA。 此外，本发明提供具有SEQ ID No.5的核苷酸序列的DNA以及具有序列表的SEQ ID No.7的核苷酸序列的DNA作为能够编码具有DHDPS的蛋白质的DNA 活动。 当在其中引入已经携带有其中含有本发明的DNA的DNA片段的重组载体进行培养时，其插入在所述重组载体的启动子的下游，可以从所述植物细胞再生 具有高赖氨酸含量的转基因植物。

公开(公告)号：EP2365071A1

公开(公告)日：2011-09-14

申请号：EP09829144.6

申请日：2009-11-26

申请人： Hokko Chemical Industry Co., Ltd.

发明人： TERAKAWA, Teruhiko ,  HASEGAWA, Hisakazu ,  ISHIMOTO, Masao ,  UTSUMI, Yasuko ,  SHOJI, Mikio ,  KAWARABAYASHI, Takeshi

IPC分类号： C12N15/00 ,  A01H5/00 ,  A61K39/00 ,  A61P25/28 ,  C12N5/10 ,  C12N15/09

CPC分类号： C12N15/8258 ,  A61K39/0005 ,  A61K2039/517 ,  C07K16/18 ,  C07K2317/13 ,  C07K2317/34

摘要： A transformed soybean plant having a gene encoding a modified seed storage protein introduced therein, obtained by inserting a gene encoding an Alzheimer's disease vaccine to a variable region(s) of a gene encoding a wild-type seed storage protein, is produced, and said vaccine is produced and accumulated in the seeds thereof.

摘要翻译： 制备具有编码经过改良的种子储存蛋白的基因的转化大豆植物，其通过将编码阿尔茨海默氏病疫苗的基因插入编码野生型种子保存蛋白质的基因的可变区而获得，并且所述 疫苗在其种子中产生并积累。

公开(公告)号：EP1026249A1

公开(公告)日：2000-08-09

申请号：EP98940641.8

申请日：1998-08-31

申请人： HOKKO CHEMICAL INDUSTRY CO. LTD. ,  Japan as repres. by Director General of National Agriculture Research Center, Ministry of Agriculture, Forestry and Fisheries

发明人： WAKASA, Kyo ,  TOZAWA, Yuzuru ,  TERAKAWA, Teruhiko ,  HASEGAWA, Hisakazu

IPC分类号： C12N15/60 ,  A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N1/21

CPC分类号： C12N15/8254 ,  C12N9/88 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/8216 ,  C12N15/8251 ,  C12Y401/03027

摘要： This invention provides a DNA sequence capable of encoding an enzyme protein which is the α-subunit of the first isozyme of rice anthranilate synthase (ASA); and a DNA capable of encoding an enzyme protein which is the α-subunit of the second isozyme of ASA. Transformed plants having a high tryptophan content can be regenerated by culturing the plant cells having introduced therein such a recombinant vector which contains, at a position downstream of a promoter, an inserted DNA fragment carrying the above DNA sequence.
This invention also provides a DNA having a promoter activity for the rice ASA gene, which DNA can be used as a promoter for introducing a useful exogenous gene into rice or other plants and expressing the gene therein.

摘要翻译： 本发明提供了能够编码蛋白质的所有这是稻邻氨基苯甲酸合酶的同功酶第一（ASA）的α亚单位的酶的DNA序列; 和能够编码蛋白质的所有这是ASA的第二同工酶的α亚单位的酶的DNA。 装载上述DNA序列插入的DNA片段的具有高色氨酸含量的转化植物可以通过培养导入了的植物细胞。其中寻求其中含有的重组载体，在启动子的下游的位置处被再生。 本发明因此提供了一种具有用于稻ASA基因，该基因的DNA可被用作用于将有用的外源基因导入水稻或其他植物及所表达该基因的启动子的启动子活性的DNA。