摘要:
The present invention relates to a method for producing cellulases and hemicellulases by a strain belonging to a filamentous fungus, in an agitated and aerated bioreactor including at least two phases, i.e. a phase a) for growing said strain in the presence of at least one carbon growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbon growth substrate of between 10 and 90 g/L, a phase b) for the continuous production of cellulases, in which at least one carbon inducing substrate is fed at an at least constant feed rate for a period at least greater than 200 hours, said carbon substrate being at least an aqueous hemicellulose hydrolysate solution derived from an acid pretreatment of a lignocellulose substrate, said aqueous hemicellulose hydrolysate solution not being presterilized the pH thereof not being corrected, said pH of the aqueous solution being between 0.5 and 3, wherein the weight of the reaction volume is kept constant by drawing off a fraction of said reaction volume, said phase b) being carried out at a dilution rate of between 0.001 and 0.02 h-1.
摘要:
The present invention concerns a method for producing sophorose from a sophorolipid which involves carrying out a step of hydrolysis of the sophorolipid in an aqueous medium in the presence of an acid compound.
摘要:
La présente invention concerne un procédé de production d'enzymes cellulolytiques et/ou hémicellulolytiques par une souche de microorganisme appartenant à la famille des champignons filamenteux, ledit procédé comprenant les étapes suivantes : (a) une première étape de croissance des champignons dans un milieu de culture aqueux, en présence d'au moins un substrat carboné de croissance, dans un bioréacteur agité et aéré, (b) une deuxième étape de production d'enzymes, à partir du milieu de culture aqueux obtenu à la première étape (a), en présence d'au moins un substrat carboné inducteur, induisant également la production d'hydrophobines, et on réintroduit dans une étape (d) au moins une partie des hydrophobines produites à l'étape (b) dans l'étape (a) de croissance.
摘要:
The invention relates to a method for the production of cellulases by a strain of a filamentous fungus in a stirred and aerated bioreactor, comprising at least two growth steps, namely: a first growth step in the presence of at least one carbon growth substrate in batch phase with a concentration of carbon growth substrate of between 10 and 80 g/L; and a second growth and production step in the presence of at least one inducing carbon substrate in fed-batch phase in the presence of a carbon-source-limiting flow of between 50 and 140 mg per gram of cells per hour.
摘要:
The invention relates to a method for producing an enzyme cocktail using a cellulolytic microorganism, including two steps: a step a) for growing said microorganism in a closed reactor in the presence of a carbon growth solution; and a step b) for producing said enzyme cocktail carried out by supplying a carbon production solution, the carbon substrate concentration of which is between 150 and 400 g/L, said carbon production solution including an inductive carbon substrate, characterized in that said inductive carbon substrate is a liquid residue from a step of pretreating lignocellulosic materials, the C5 sugar oligomers of which are at least 1 wt % of the total sugars in said liquid residue and at least 0.3 wt % of the total sugars in said carbon production solution.