公开(公告)号：EP3037554B1

公开(公告)日：2018-08-01

申请号：EP16151156.3

申请日：2010-04-09

申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  UNIVERSITE DE MONTPELLIER ,  Centre Hospitalier Universitaire de Montpellier

发明人： HAMAMAH, Samir ,  DE VOS, John ,  ASSOU, Said

IPC分类号： G01N33/68 ,  C12Q1/6881

CPC分类号： C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/689

摘要： The present invention relates to a method for selecting an oocyte that when fertilized produces a viable embryo with a high implantation rate leading to pregnancy by measuring, in a cumulus cell surrounding said oocyte, the expression level of 45 specific genes which consist in WNT6, LRCH4, PAX8, CABP4, PDE5A, BCL2L11, PCK1, TCF20, SLAMF6, EPOR, CACNG6, NLRP1, PECAM1, NOS1, ATF3, KRTAP8, GRIK5, SLC24A3, SLC5A12, SLCA10A2, SLCO1A2, SLC25A5, MG29, NLGN2, PRKACA, FOSB, SIAT6, LOXL2, PRF1, ADPRH, APBB3, EGR3, CNR2, IFITM1, PLA2G5, CAMTA1, SOX4, NFIB, NFIC, RBMS1, G0S2, FAT3, SLC40A1, GPC6 and IGF1R

公开(公告)号：EP3011047B1

公开(公告)日：2018-08-01

申请号：EP14731624.4

申请日：2014-06-18

申请人： INSERM - Institut National de la Santé et de la Recherche Médicale ,  UNIVERSITE DE MONTPELLIER ,  Centre Hospitalier Universitaire de Montpellier ,  Institut Regional du Cancer de Montpellier

发明人： HAMAMAH, Samir ,  EL MESSAOUDI, Safia ,  THIERRY, Alain ,  ASSOU, Said

IPC分类号： C12Q1/6883

CPC分类号： C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158

摘要： The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to in vitro non invasive methods and kits for determining the quality of an embryo by determining the level of the cell free nucleic acids and/or determining the presence and/or expression level of at least one specific nucleic acid sequence in the nude is acid extraction.

公开(公告)号：EP3011047A1

公开(公告)日：2016-04-27

申请号：EP14731624.4

申请日：2014-06-18

申请人： INSERM - Institut National de la Santé et de la Recherche Médicale ,  UNIVERSITE DE MONTPELLIER ,  Centre Hospitalier Universitaire de Montpellier ,  Institut Regional du Cancer de Montpellier

发明人： HAMAMAH, Samir ,  EL MESSAOUDI, Safia ,  THIERRY, Alain ,  ASSOU, Said

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158

摘要： The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to in vitro non invasive methods and kits for determining the quality of an embryo by determining the level of the cell free nucleic acids and/or determining the presence and/or expression level of at least one specific nucleic acid sequence in the nude is acid extraction.

摘要翻译： 本发明一般涉及生殖医学领域。 更具体地，本发明涉及用于通过确定无细胞核酸的水平和/或确定至少一种特定核酸的存在和/或表达水平来确定胚胎质量的体外非侵入性方法和试剂盒 裸体序列是酸提取。

公开(公告)号：EP2726630A1

公开(公告)日：2014-05-07

申请号：EP12730558.9

申请日：2012-06-29

申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  Universite de Montpellier 1 ,  Centre Hospitalier Universitaire de Montpellier

发明人： HAMAMAH, Samir ,  ASSOU, Said ,  OUANDAOGO ZAMALOU, Gisèle ,  HAOUZI, Delphine

IPC分类号： C12Q1/68

CPC分类号： C12N5/0605 ,  C12N5/0682 ,  C12N2501/115 ,  C12N2533/54 ,  C12Q1/6881

摘要： The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to a method determining the developmental stage of human cumulus cells issues from MII oocyte.

公开(公告)号：EP3037554A1

公开(公告)日：2016-06-29

申请号：EP16151156.3

申请日：2010-04-09

申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  UNIVERSITE DE MONTPELLIER ,  Centre Hospitalier Universitaire de Montpellier

发明人： HAMAMAH, Samir ,  DE VOS, John ,  ASSOU, Said

IPC分类号： C12Q1/68 ,  G01N33/68 ,  G01N33/569

CPC分类号： C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/689

摘要： The present invention relates to a method for selecting an oocyte that when fertilized produces a viable embryo with a high implantation rate leading to pregnancy by measuring, in a cumulus cell surrounding said oocyte, the expression level of 45 specific genes which consist in WNT6, LRCH4, PAX8, CABP4, PDE5A, BCL2L11, PCK1, TCF20, SLAMF6, EPOR, CACNG6, NLRP1, PECAM1, NOS1, ATF3, KRTAP8, GRIK5, SLC24A3, SLC5A12, SLCA10A2, SLCO1A2, SLC25A5, MG29, NLGN2, PRKACA, FOSB, SIAT6, LOXL2, PRF1, ADPRH, APBB3, EGR3, CNR2, IFITM1, PLA2G5, CAMTA1, SOX4, NFIB, NFIC, RBMS1, G0S2, FAT3, SLC40A1, GPC6 and IGF1R

摘要翻译： 本发明涉及一种方法，用于选择的卵母细胞的速率并当受精产生一个与高注入通过测量导致妊娠一个存活的胚胎，在卵丘细胞包围所述卵母细胞，在45个特定基因的表达水平，其由在WNT6，LRCH4 ，PAX8，CABP4，PDE5A，BCL2L11，PCK1，TCF20，SLAMF6，EPOR，CACNG6，NLRP1，PECAM1，NOS1，ATF3，KRTAP8，GRIK5，SLC24A3，SLC5A12，SLCA10A2，SLCO1A2，SLC25A5，MG29，NLGN2，PRKACA，FOSB，SIAT6 ，LOXL2，PRF1，ADPRH，APBB3，EGR3，CNR2，IFITM1，PLA2G5，CAMTA1，SOX4，NFIB，NFIC，RBMS1，GOS2启动，FAT-3，SLC40A1，GPC6和IGF1R

公开(公告)号：EP3347487A1

公开(公告)日：2018-07-18

申请号：EP16763789.1

申请日：2016-09-09

申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  Université de Montpellier ,  Centre Hospitalier Universitaire de Montpellier

发明人： DE VOS, John ,  ASSOU, Said ,  GIRAULT, Nicolas

IPC分类号： C12Q1/68 ,  A61K35/545 ,  C12N5/0735

CPC分类号： C12N5/0606 ,  A61K35/545 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158

摘要： The present invention relates to a novel non-invasive method for assessing pluripotent stem cells quality in culture. More specifically, the present invention relates to a non-invasive method for assessing genetic integrity (such as the presence of CNVs) of pluripotent stem cells in culture, by assessing cell-free nucleic acids in the supernatant of the cell culture.

公开(公告)号：EP2419526B1

公开(公告)日：2016-03-30

申请号：EP10713218.5

申请日：2010-04-09

申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  Université de Montpellier ,  Centre Hospitalier Universitaire de Montpellier

发明人： HAMAMAH, Samir ,  DE VOS, John ,  ASSOU, Said

IPC分类号： C12Q1/68 ,  G01N33/569

CPC分类号： C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/689

公开(公告)号：EP2603581B1

公开(公告)日：2019-07-17

申请号：EP11741581.0

申请日：2011-08-11

申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  Université de Montpellier ,  Centre Hospitalier Universitaire de Montpellier

发明人： HAMAMAH, Samir ,  ASSOU, Said

IPC分类号： C12N5/073 ,  C12N5/0735 ,  A61D19/00

公开(公告)号：EP2603581A1

公开(公告)日：2013-06-19

申请号：EP11741581.0

申请日：2011-08-11

申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  Université de Montpellier I ,  Centre Hospitalier Universitaire de Montpellier

发明人： HAMAMAH, Samir ,  ASSOU, Said

IPC分类号： C12N5/073 ,  C12N5/0735 ,  A61D19/00

CPC分类号： C12N5/0603 ,  A61D19/00 ,  C12N5/0604 ,  C12N5/0605 ,  C12N5/0606 ,  C12N2502/02 ,  C12N2517/10

摘要： The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to a novel human embryo co-culture system to improve human embryo growth in vitro and, consequently, increase pregnancy rates in infertile women undergoing in vitro fertilization (IVF) treatment. More particularly, the present invention relates to a method of growing an embryo to a blastocyst stage of development comprising the step of coculturing said embryo in the presence of a population of cumulus cells.

公开(公告)号：EP2643478B1

公开(公告)日：2018-01-03

申请号：EP11794430.6

申请日：2011-11-24

申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  Centre Hospitalier Universitaire de Montpellier ,  Université de Montpellier

发明人： HAMAMAH, Samir ,  ASSOU, Said

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6876 ,  C12N2310/141 ,  C12N2320/10 ,  C12Q1/6881 ,  C12Q1/6883 ,  C12Q2600/158 ,  C12Q2600/178

摘要： The present invention relates to a method for selecting a competent oocyte or a competent embryo by determining the expression level of specific microRNA species in a body fluid or in cumulus cells.